aerobic-biodegradation-testing-materials-using-natural-marine

Aerobic Biodegradation Testing of Materials Using a Natural Marine Seawater Inoculum and Closed Loop Respirometer

Proper assessment of biodegradable materials depends on the wide adoption of standard test methods. Although such methods exist, testing capacity remains ...

University of Massachusetts

Protein fouling on critical biointerfaces causes significant public health and clinical ramifications. Multiple strategies, including superhydrophobic (SHP) surfaces and coatings, have been explored to mitigate protein adsorption on solid surfaces. SHP materials with underwater air plastron (AP) layers hold great promise by physically reducing the contact area between a substrate and protein molecules. However, sustaining AP stability or lifetime is crucial in determining the durability and long-term applications of SHP materials. This work investigated the effect of protein on the AP stability using model SHP substrates, which were prepared from a mixture of silica nanoparticles and epoxy. The AP stability was determined using a submersion test with real-time visualization. The results showed that AP stability was significantly weakened by protein solutions compared to water, which could be attributed to the surface tension of protein solutions and protein adsorption on SHP substrates. The results were further examined to reveal the correlation between protein fouling and accelerated AP dissipation on SHP materials by confocal fluorescent imaging, surface energy measurement, and surface robustness modeling of the Cassie-Baxter to Wenzel transition. The study reveals fundamental protein adsorption mechanisms on SHP materials, which could guide future SHP material design to better mitigate protein fouling on critical biointerfaces.

Effect of Protein Adsorption on Air Plastron Behavior of a Superhydrophobic Surface.

The effect of particle loading on the wetting properties of coatings was investigated by modifying a coating formulation based on hydrophilic silica nanoparticles and poly (acrylic acid) (PAA). Water contact angle (WCA) measurements were conducted for all coatings to characterize the surface wetting properties. Wettability was improved with an increase in particle loading. The resulting coatings showed superhydrophilic (SH) behavior when the particle loading was above 53 vol. %. No new peaks were detected by attenuated total reflection (ATR-FTIR). The surface topography of the coatings was studied by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of hydrophilic functional groups and nano-scale roughness were found to be responsible for superhydrophilic behavior. The surface chemistry was found to be a primary factor determining the wetting properties of the coatings. Adhesion of the coatings to the substrate was tested by tape test and found to be durable. The antifogging properties of the coatings were evaluated by exposing the films under different environmental conditions. The SH coatings showed anti-fogging behavior. The transparency of the coatings was significantly improved with the increase in particle loading. The coatings showed good transparency (>85% transmission) when the particle loading was above 84 vol. %.

Wetting Characteristics of Nanosilica-Poly (acrylic acid) Transparent Anti-Fog Coatings.

Superhydrophilic coatings based on a hydrophilic silica nanoparticle suspension and Poly (acrylic acid) (PAA) were prepared by dip coating. Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) were used to examine the morphology of the coating. The effect of surface morphology on the dynamic wetting behavior of the superhydrophilic coatings was studied by changing the silica suspension concentration from 0.5% wt. to 3.2% wt. while keeping the silica concentration in the dry coating constant. The droplet base diameter and dynamic contact angle with respect to time were measured using a high-speed camera. A power law was found to describe the relationship between the droplet diameter and time. A significantly low experimental power law index was obtained for all the coatings. Both roughness and volume loss during spreading were suggested to be responsible for the low index values. The water adsorption of the coatings was found to be the reason for the volume loss during spreading. The coatings exhibited good adherence to the substrates and retention of hydrophilic properties under mild abrasion.

Article	Year
Effect of Protein Adsorption on Air Plastron Behavior of a Superhydrophobic Surface. ACS applied materials & interfaces\| PubMed ID: 34813281	2021
Wetting Characteristics of Nanosilica-Poly (acrylic acid) Transparent Anti-Fog Coatings. Polymers\| PubMed ID: 36365655	2022
Dynamic Wetting Properties of Silica-Poly (Acrylic Acid) Superhydrophilic Coatings. Polymers\| PubMed ID: 36904483	2023

Jo Ann Ratto

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