integrated-pcr-based-molecular-detection-system-for-simultaneous

Integrated PCR-Based Molecular Detection System for the Simultaneous Detection of Four Zoonotic Intestinal Parasites from Multiple Sources

Infectious diseases are critical factors affecting human health, economic development, and social stability. Among them, zoonotic intestinal parasitic ...

Yancheng Center for Disease Control and Prevention

To better understand the spectrums of pathogens causing herpangina and circulation of Coxsackievirus A4 in Yancheng, China.

Mosaic genome of Human Coxsackievirus A4 associated with herpangina and HFMD in Yancheng, China, 2016 and 2018.

Asymptomatic and Symptomatic Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infections in Close Contacts of Coronavirus Disease 2019 (COVID-19) Patients: A Seroepidemiological Study.

Rodents are the primary natural reservoirs of  spp., and some of which are zoonotic causative agents. Hence, surveillance of  sp. infection in rodents is very important for the prevention of human bartonellosis caused by them. In this study, rodents were captured, and their spleen samples were collected for  sp. DNA detection and identification by amplifying the 16S rRNA, , and  genes using semi-nested polymerase chain reaction (PCR). The results indicated that  sp. DNA was detected in seven  individuals with a detection rate of 6.7% in Chengde City and bacterial DNA in 31  individuals with a detection rate of 28.4% in Handan City. The DNA detection rate across the genders and ages of rodents was not found to be statistically significant. Furthermore, sequence analysis of the above-mentioned three genes demonstrated that at least eight  species were circulating in Hebei Province, of which three, including , , and , are human pathogens, thus suggesting the existence of a major public health risk. Overall, these results revealed the detection rate and genetic diversity of  species infection in rodents in Hebei Province, which could be potentially helpful for the prevention of bartonellosis caused by rodent-associated  species. This study highlights the urgent need for the surveillance of  infections in rodents and ectoparasites that affect both rodents and humans and can cause fever of unknown origin or endocarditis.

Genetic diversity of  infection in residential and field rodents in Hebei, China.

China was affected severely by tick-borne rickettsiosis, and more than 10 Rickettsia species pathogenic to humans have been identified. In recent years, several Rickettsia members, with unknown pathogenicity, firstly identified abroad have been found in China. In this study, parasitic and questing ticks were recovered from two sampling sites in Hebei, China. Specific primers targeting outer membrane protein B (ompB) gene were designed to test the presence of Rickettsia canadensis by nested Polymerase Chain Reaction (PCR). As a result, a total of 428 ticks, including 232 ticks (including 230 Haemaphysalis longicornis and two H. japonica) from Laiyuan County and 196 (H. longicornis) from Luanping County, were collected. Sequencing of PCR products with the expected size and subsequently BLAST showed that 38H. longicornis ticks tested positive for R. canadensis, with an overall positive rate of 8.8%. In addition, 800-bp ompB gene and nearly complete citrate synthase (gltA) gene were recovered from six randomly selected positive samples to better understand their genetic characteristics. Nucleotide identity and phylogenetic analyses showed that R. canadensis presented geographical clustering with evidence that variants identified in the current study presented closer genetic relationship with others identified in Asian than those found in North America. In addition, epidemiological data suggested that H. longicornis may be the competent vector, and more attention should be paid to R. canadensis due to its zoonotic potential. In sum, R. canadensis was confirmed to be present in Hebei Province, China, and its surveillance in ticks should be strengthened due to potential pathogenicity, higher positive rate in ticks and wide distribution of possible vector tick species.

Molecular evidence of Rickettsia canadensis in ticks, Hebei, China.

Rodents are recognized as the hosts of many vector-borne bacteria and protozoan parasites and play an important role in their transmission and maintenance. Intensive studies have focused on their infections in vectors, especially in ticks, however, vector-borne bacterial and protozoan infections in rodents are poorly understood although human cases presenting with fever may due to their infection have been found.

Article	Year
Mosaic genome of Human Coxsackievirus A4 associated with herpangina and HFMD in Yancheng, China, 2016 and 2018. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases\| PubMed ID: 32439540	2020
Asymptomatic and Symptomatic Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infections in Close Contacts of Coronavirus Disease 2019 (COVID-19) Patients: A Seroepidemiological ... Clinical infectious diseases : an official publication of the Infectious Diseases Society of America\| PubMed ID: 32544949	2021
Genetic diversity of infection in residential and field rodents in Hebei, China. Frontiers in microbiology\| PubMed ID: 36504836	2022
Molecular evidence of Rickettsia canadensis in ticks, Hebei, China. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases\| PubMed ID: 37742909	2023
Great genetic diversity of vector-borne bacteria and protozoan in wild rodents from Guangxi, China. PLoS neglected tropical diseases\| PubMed ID: 38739673	2024

Guoqing Chen

Biography

JoVE Journal Publications

Other Publications