effect-tooth-position-on-molar-zirconia-crown-restoration-with

Effect of Tooth Position on Molar Zirconia Crown Restoration with a Modified Dental Measurement Software

This study employed a modified digital technique to evaluate parameters of molar preparations for monolithic zirconia crown restoration, investigating ...

Capital Medical University

To investigate the effect of recombinant human tumor necrosis factor alpha (rhTNF-α) on the osteogenesis potential of the osteo-induced human adipose-derived stromal cells (hASCs) in vitro.

[Recombinant human tumor necrosis factor-alpha promotes human adipose-derived stromal cells transforming into osteoblast in vitro].

Cisplatin (DDP) is the first-line chemotherapy agent for the treatment of oral squamous cell carcinoma (OSCC). The emergence of DDP resistance leads to diminished drug efficacy and survival benefit. lncRNA MALAT1 has been considered as one of the most important factors in OSCC. It has also been reported to enhance chemo-resistance in other kinds of carcinomas. However, little is known about the role of lncRNA MALAT1 in DDP resistance of OSCC.

lncRNA MALAT1 Promotes EMT Process and Cisplatin Resistance of Oral Squamous Cell Carcinoma via PI3K/AKT/m-TOR Signal Pathway.

Oral cancer (OC) is one of the most common cancers around the world. Despite the progress in treatment, the prognosis of OC remains poor, especially for patients with advanced diseases. It urges the development of novel therapeutic options against OC. Lycopene (LYC) is an antioxidant with chemoprotective properties against cancer. However, little is known about the mechanisms underlying the protective role of LYC in OC tumorigenesis.

Lycopene Inhibits Epithelial-Mesenchymal Transition and Promotes Apoptosis in Oral Cancer via PI3K/AKT/m-TOR Signal Pathway.

Head and neck squamous cell carcinoma (HNSCC) is recognised as an immune active cancer, but little is known about the role of microRNAs (miRNAs) in it. In the present study, we aim to determine a prognostic and immune-related miRNAs signature (IRMS) in HNSCC.

Immune-related miRNA signature identifies prognosis and immune landscape in head and neck squamous cell carcinomas.

Commonly recognized mechanisms of the xenogeneic-extracellular matrix-based regenerative medicine include timely degradation, release of bioactive molecules, induced differentiation of stem cells, and well-controlled inflammation. This process is most feasible for stromal tissue reconstruction, yet unsuitable for non-degradable scaffold and prefabricated-shaped tissue regeneration, like odontogenesis. Treated dentin matrix (TDM) has been identified as a bioactive scaffold for dentin regeneration. This study explored xenogeneic porcine TDM (pTDM) for induced odontogenesis. The biological characteristics of pTDM were compared with human TDM (hTDM). To investigate its bioinductive capacities on allogeneic dental follicle cells (DFCs) in the inflammation microenvironment, pTDM populated with human DFCs were co-cultured with human peripheral blood mononuclear cells (hPBMCs), and pTDM populated with rat DFCs were transplanted into rat subcutaneous model. The results showed pTDM possessed similar mineral phases and bioactive molecules with hTDM. hDFCs, under the induction of pTDM and hTDM, expressed similar col-I, osteopontin and alkaline phosphatase (ALP) (all expressed by odontoblasts). Whereas, the expression of col-I, dentin matrix protein-1 (DMP-1) and bone sialoprotein (BSP) were down-regulated when cocultured with hPBMCs. The xenogeneic implants inevitably initiated Th1 inflammation (up-regulated CD8, TNF-α, IL-1, etc). However, the biomineralization of pre-dentin and cementum were still processed, and collagen fibrils, odontoblast-like cells, fibroblasts contributed to odontogenesis. Although partially absorbed at 3 weeks, the implants were positively expressed odontogenesis-related-proteins like col-I and DMP-1. Taken together, xenogeneic TDM conserved ultrastructure and molecules for introducing allogeneic DFCs to odontogenic differentiation, and promoting odontogenesis and biomineralization. Yet effective immunomodulation methods warrant further explorations.

Xenogeneic dentin matrix as a scaffold for biomineralization and induced odontogenesis.

Relationship between lipid profile and periodontitis has been reported. However, the association between lipid parameters and edentulism is unclear. This study aimed to investigate the association between lipid profile and reported edentulism in the elder population using a national cohort.

Association of lipid profile and reported edentulism in the elder population: data from the China Health and Retirement Longitudinal Study.

 Members of a disintegrin and metalloproteinase (ADAM) family play a vital role in cancer development. However, a comprehensive analysis of the landscape of the ADAM family in pan-cancer remains to be performed.  The correlation of the expression level and prognostic value with ADAMs in a pan-cancer cohort and the relationship between ADAMs and the stemness score, tumour microenvironment (TME), chemotherapy-related drug sensitivity, immune subtype, and immunotherapy outcome were investigated.  ADAMs were differentially expressed between tumour and para-carcinoma tissues in the pan-cancer cohort, and the expression of ADAMs was significantly correlated with patient prognosis. Furthermore, ADAMs were significantly correlated with the stromal score and immune score based on the TME analysis. Additionally, ADAMs were also correlated with DNAss and RNAss in the pan-cancer cohort. On investigating the CellMiner database, ADAMs were revealed to be significantly correlated with the sensitivity of various drugs, including raloxifene and tamoxifen. Moreover, in the IMvigor210 and GSE78220 cohorts, ADAMs were correlated with immunotherapy response and immune activation genes. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were utilised to determine the differential level of ADAM9 in cancer and para-carcinoma tissues in patients' samples.  This study elucidates the importance of ADAMs in cancer progression and lays a foundation for further exploration of ADAMs as potential pan-cancer targets.

Pan-cancer analysis of ADAMs: A promising biomarker for prognosis and response to chemotherapy and immunotherapy.

Both the absence of autophagy and excessive autophagy is double-edged sword in tumorigenesis. Due to the specificity of autophagy, its role in head and neck squamous cell carcinoma (HNSCC) is still unclear. In this study, we established five autophagy-related patterns in 1165 HNSCC patients with distinct cellular and molecular characteristics. Additionally, we developed a new scoring system (ATPscore) based on the differentially expressed genes (DEGs) among these five patterns, to represent the individual autophagy regulation pattern. ATPscore was shown to be significantly correlated with tumor immune microenvironment (TIME) infiltration, immune phenotypes, molecular subtypes, and genetic variations. We further found that ATPscore was both an independent prognostic factor and a potent predictor of clinical response to immune-checkpoint inhibitors (ICIs) based immunotherapy. We further verified the value of key gene SRPX in ATPscore in HNSCC cell lines with the in-depth research of ATPscore and found that it is closely related to immune subtypes, molecular subtypes, and immune activation-related markers. Our research could help us to understand the underlying mechanisms of tumor immunity and provide a solid foundation for combination of autophagy-targeted therapies with immunotherapies for clinical application in HNSCC.

A novel autophagy-related subtypes to distinguish immune phenotypes and predict immunotherapy response in head and neck squamous cell carcinoma.

Mesenchymal stem cell (MSC)-based therapies have emerged as a promising approach for treating articular cartilage injuries. However, enhancing the chondrogenic differentiation potential of MSCs remains a significant challenge. KDM6B, a histone demethylase that specifically removes H3K27me3 marks, is essential in controlling the maturation of chondrocytes. In this study, we examined how KDM6B influences chondrogenic differentiation in SCAPs and investigated the underlying mechanisms involved.

Histone Demethylase KDM6B Promotes Chondrogenic Differentiation Potential of Stem Cells from the Apical Papilla via HES1.

The cartilage tissue regeneration mediated with mesenchymal stem cells (MSCs) is considered as a viable strategy for temporomandibular joint osteoarthritis (TMJOA). Betaine has been confirmed to modulate the multidirectional differentiation of MSCs, while its effect on chondrogenic differentiation of Stem Cells from the Apical Papilla (SCAPs) is unknown. Here, we explored the effects and underlying mechanisms of betaine on chondrogenic differentiation of SCAPs.

Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81.

Functional tissue repair is often constrained by inflammation and fibrosis. Alternatively activated M2 macrophages have emerged as promising therapeutic targets for optimizing graft-to-host interactions; however, efficient induction methods are required. Presumably, the outcome of regenerative wound healing or scar formation/fibrosis might be dependent on the balance between M2a and M2c sub-phenotypes. This study utilized dual-functionalized graphene oxide (GO) as a DNA delivery agent to induce M2a and M2c macrophage polarization. Mechanistically, molecular characteristics were analyzed using RNA sequencing. We designed GO with polyethyleneimine (PEI) modification and subsequently conjugated it with polyethylene glycol (PEG)-folate (FA) to target human THP-1-derived macrophage activation. Specifically, the resulting GO-PEI-PEG-FA (GPPF) compound effectively activated CD206CD209M2a and CD163MerTKM2c phenotype polarization. The efficient delivery of IL4 or IL10 plasmid DNA using GPPF (GPPF/pIL4 or GPPF/pIL10) significantly enhanced macrophage cellular elongation and reduced MHC-II-associated antigen presentation. M2a(GPPF/pIL4) and M2c(GPPF/pIL10) were validated as negative regulators of the immune response and positive regulators of Th2 effectors. Up-regulated genes in M2a(GPPF/pIL4) even inhibited type I interferon production and restricted the innate immune response. Supplemental to the established data, M2a(GPPF/pIL4) behaved similar to IFN-responsive macrophages, restricting viral life cycles and promoting myogenesis and osteogenesis. Meanwhile, M2c(GPPF/pIL10) was characterized using IL10 signaling, anti-fibrosis, and neutrophil-mediated suppression of the LPS-bacterial response. Regarding the tissue remodeling process, the two subsets attenuated negative-regulated BMP signaling to facilitate osteoinduction and up-regulated NAMPT to establish a transient stem cell-activating niche for tissue regeneration. This study underscored the potential of functionalized GO-induced M2 sub-phenotypes as modulators in regenerative medicine.

Article	Year
[Recombinant human tumor necrosis factor-alpha promotes human adipose-derived stromal cells transforming into osteoblast in vitro]. Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences\| PubMed ID: 22692324	2012
lncRNA MALAT1 Promotes EMT Process and Cisplatin Resistance of Oral Squamous Cell Carcinoma via PI3K/AKT/m-TOR Signal Pathway. OncoTargets and therapy\| PubMed ID: 32494159	2020
Lycopene Inhibits Epithelial-Mesenchymal Transition and Promotes Apoptosis in Oral Cancer via PI3K/AKT/m-TOR Signal Pathway. Drug design, development and therapy\| PubMed ID: 32606612	2020
Immune-related miRNA signature identifies prognosis and immune landscape in head and neck squamous cell carcinomas. Bioscience reports\| PubMed ID: 33111959	2020
Xenogeneic dentin matrix as a scaffold for biomineralization and induced odontogenesis. Biomedical materials (Bristol, England)\| PubMed ID: 33902010	2021
Association of lipid profile and reported edentulism in the elder population: data from the China Health and Retirement Longitudinal Study. BMC oral health\| PubMed ID: 36243707	2022
Pan-cancer analysis of ADAMs: A promising biomarker for prognosis and response to chemotherapy and immunotherapy. Frontiers in genetics\| PubMed ID: 37082201	2023
A novel autophagy-related subtypes to distinguish immune phenotypes and predict immunotherapy response in head and neck squamous cell carcinoma. Biomolecules & biomedicine\| PubMed ID: 37270839	2023
Histone Demethylase KDM6B Promotes Chondrogenic Differentiation Potential of Stem Cells from the Apical Papilla via HES1. Cells, tissues, organs\| PubMed ID: 39799938	2025
Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81. Stem cell research & therapy\| PubMed ID: 39920811	2025
Dual-functionalized graphene oxide induces M2a and M2c macrophage polarization to orchestrate inflammation and tissue remodeling. Journal of materials chemistry. B\| PubMed ID: 40613626	2025

Riyue Yu

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