surgical-protocols-for-deep-cervical-lymphovenous-anastomosis-rat

Surgical Protocols for Deep Cervical Lymphovenous Anastomosis in a Rat Model: Lymph Node and Lymphatic Vessel Anastomoses

Deep cervical lymphovenous anastomosis (dcLVA) has become a promising treatment strategy for Alzheimer&#39;s disease (AD), offering significant ...

The Second Affiliated Hospital of Harbin Medical University

Low-grade gliomas (LGG) are WHO grade II tumors presenting as the most common primary malignant brain tumors in adults. Currently, LGG treatment involves either or a combination of surgery, radiation therapy, and chemotherapy. Despite the knowledge of constitutive genetic risk factors contributing to gliomas, the role of single genes as diagnostic and prognostic biomarkers is limited. The aim of the current study is to discover the predictive and prognostic genetic markers for LGG.

New biomarker: the gene HLA-DRA associated with low-grade glioma prognosis.

Glioma is the most common and fatal primary brain tumor in humans. A significant role for long non-coding RNA (lncRNA) in glioma is the regulation of gene expression and chromatin recombination, and immunotherapy is a promising cancer treatment. Therefore, it is necessary to identify necroptosis-related lncRNAs in glioma. In this study, we collected and evaluated the RNA-sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA, https://www.ncbi.nlm.nih.gov/, Data Release 32.0, March 29, 2022) glioma patients, and necroptosis-related lncRNAs were screened. Cox regression and least absolute shrinkage and selection operator (LASSO) analysis were performed to construct a risk score formula to explore the different overall survival between high- and low-risk groups in TCGA. Gene Ontology (GO) and pathway enrichment analysis (Kyoto Encyclopedia of Genes and Genomes (KEGG)) were performed to identify the function of screened genes. The immune correlation analysis showed that various immune cells and pathways positively associated with a patient's risk score. Furthermore, the analysis of the tumor microenvironment indicated many immune cells and stromal cells in the tumor microenvironment of glioma patients. Six necroptosis-related lncRNAs were concerned to be involved in survival and adopted to construct the risk score formula. The results showed that patients with high-risk scores held poor survival in TCGA. Compared with current clinical data, the area under the curve (AUC) of different years suggested that the formula had better predictive power. We verified that necroptosis-related lncRNAs play a significant role in the occurrence and development of glioma, and the constructed risk model can reasonably predict the prognosis of glioma. The results of these studies added some valuable guidance to understanding glioma pathogenesis and treatment, and these necroptosis-related lncRNAs may be used as biomarkers and therapeutic targets for glioma prevention.

Comprehensive analysis: Necroptosis-related lncRNAs can effectively predict the prognosis of glioma patients.

To analyze the expression of TXLNA in brain gliomas and its clinical significance.

Expression of TXLNA in brain gliomas and its clinical significance: a bioinformatics analysis.

Extracellular matrix (ECM), a core member of tumor microenvironment, is ≈1.5-fold harder than the surrounding normal tissues. Regulating the stiffness of ECM can significantly impact physiological activities of tumor cells, such as growth, differentiation, and migration. Herein, a sonopiezoelectric-response nanoplatform consisting of CuBiS nanospheres (CBS NSs) is constructed for ECM remodeling. Sonopiezoelectric therapy (SPT) and chemodynamic therapy (CDT) are conducted using ultrasound (US) and near-infrared irradiation. Under US irradiation, the mechanical strain of CBS NSs causes piezoelectric polarization and promotes a redox reaction through energy band bending. The built-in electric field generated by US irradiation amplifies the efficiency of the Fenton-like reaction and substantially enhances reactive oxygen species production. Moreover, piezoelectric property-mediated electrical signals can allow Ca influx, upregulating the levels of matrix metalloproteinase (MMP)-2 and MMP-9. Integrating US irradiation with near-infrared irradiation generates localized heat, which can effectively denature tumor collagen, reduce tumor stiffness, and enhance the permeability of CBS NSs into solid tumors, thus improving the SPT effect. The combination of MMP upregulation and collagen degradation can maximize the benefits of ECM remodeling and synergistically enhance the cancer therapeutic efficacy of SPT/CDT. This SPT/CDT synergistic therapy and ECM remodeling platform is an innovative strategy for cancer therapy.

Article	Year
New biomarker: the gene HLA-DRA associated with low-grade glioma prognosis. Chinese neurosurgical journal\| PubMed ID: 35585639	2022
Comprehensive analysis: Necroptosis-related lncRNAs can effectively predict the prognosis of glioma patients. Frontiers in oncology\| PubMed ID: 36033536	2022
Expression of TXLNA in brain gliomas and its clinical significance: a bioinformatics analysis. Chinese neurosurgical journal\| PubMed ID: 37752559	2023
Dual Strategy of Ca Influx and Collagen Denaturation to Remodel the Extracellular Matrix and Amplify Sonopiezoelectric Therapy. Advanced materials (Deerfield Beach, Fla.)\| PubMed ID: 40444340	2025

Desheng Chen

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