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Microfabrication of Substrates with Microscale Stiffness Gradients to Guide Bone Marrow Stromal Cell Migration

Understanding how cells respond to mechanical signals is crucial for elucidating the mechanisms involved in tissue development and disease progression. ...

wuhan university

Chronic hepatitis B virus (HBV) infection is a major cause of hepatocellular carcinoma (HCC), pegylated-interferon-α(PEG-IFNα) and long-term nucleos(t)ide analogs (NUCs) are mainly drugs used to treat HBV infection, but the effectiveness is unsatisfactory in different populations, the exploration of novel therapeutic approaches is necessary. RAD51C is associated with DNA damage repair and plays an important role in the development and progression of tumors. Early cDNA microarray results showed that RAD51C expression was significantly increased in HBV-infected HCC cells, however, the relationship between HBV infection and abnormal expression of RAD51C has not been reported. Therefore, we conducted RT-PCR, western blot, Co-immunoprecipitation(Co-IP), and immunofluorescence(IF) to detect HBV-RAD51C interaction in RAD51C overexpression or interfering HCC cells. Our results showed that RAD51C and HBV X protein(HBX) produced a direct interaction in the nucleus, the HBV infection of HCC cells promoted RAD51C expression, and the increased expression of RAD51C promoted HBV replication. This indicated that RAD51C is closely related to the occurrence and development of HCC caused by HBV infection, and may bring a breakthrough in the the prevention and treatment study of HCC.

HBV promotes its replication by up-regulating RAD51C gene expression.

Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV.

Increased QPCT gene expression by the hepatitis B virus promotes HBV replication.

Memristors have emerged as a promising hardware platform for in-memory computing, but many current devices suffer from channel material degradation during repeated resistive switching. This leads to high energy consumption and limited endurance. Here we introduce a molecular crystal memristor, of which the representative channel material, SbO, possesses a molecular crystal structure where molecular cages are interconnected via van der Waals forces. This unique configuration allows ions to migrate through intermolecular spaces with relatively low energy input, preserving the integrity of the crystal structure even after extensive switching cycles. Our molecular crystal memristor thus exhibits low energy consumption of 26 zJ per operation, with prominent endurance surpassing 10 switching cycles. The device delivers both reconfigurable non-volatile and volatile resistive switching behaviours over a broad range of device scales, from micrometres down to nanometres. Furthermore, we establish the scalability of this technology by fabricating large crossbar arrays on an 8 inch wafer. This enables the successful implementation of reservoir computing on a single CMOS-integrated chip using these memristors, achieving 100% accuracy in dynamic vision recognition.

Article	Year
HBV promotes its replication by up-regulating RAD51C gene expression. Scientific reports\| PubMed ID: 38297111	2024
Increased QPCT gene expression by the hepatitis B virus promotes HBV replication. PloS one\| PubMed ID: 39531472	2024
Molecular crystal memristors. Nature nanotechnology\| PubMed ID: 40962872	2025

Conghui Zhang

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