integrating-continuous-renal-replacement-therapy-into-ex-situ

Integrating Continuous Renal Replacement Therapy into <em>Ex-situ</em> Normothermic Liver Machine Perfusion

Ex situ normothermic machine perfusion (NMP) of the liver has emerged as a dynamic preservation strategy, enabling the maintenance of metabolic activity ...

General Hospital of Southern Theater Command of PLA

There is a dearth of effective parameters for selecting potentially transplantable liver grafts from expanded-criteria donors. In this study, we used a nuclear magnetic resonance (NMR) relaxation analyzer-based assay to assess the viability of ex vivo livers obtained via porcine donation after circulatory death (DCD). Ex situ normothermic machine perfusion (NMP) was utilized as a platform for viability test of porcine DCD donor livers. A liver-targeted contrast agent, gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA), was injected into the perfusate during NMP, and the dynamic biliary excretion of the Gd-EOB-DTPA was monitored by measuring the longitudinal relaxation time (T1). The longitudinal relaxation rate (R1) of the bile was served as a parameter. The delay of increase in biliary R1 during early stage of NMP indicated the impaired function of liver grafts in both warm and cold ischemia injury, which was correlated with the change of alanine aminotransferase. The preservative superiority in cold ischemia of dual hypothermic oxygenated machine perfusion could also be verified by assessing biliary R1 and other biochemical parameters. This study allows for the dynamic assessment of the viability of porcine DCD donor livers by combined usage of ex situ NMP and NMR relaxation time based assay, which lays a foundation for further clinical application.

Evaluation of the ex vivo liver viability using a nuclear magnetic resonance relaxation time-based assay in a porcine machine perfusion model.

The use of grafts from donation after circulatory death (DCD) overcomes the inadequate donor organ supply. Our team developed a transportable dual hypothermic oxygenated machine perfusion (DHOPE) device, which initiates DHOPE at a recipient center to reduce static cold storage (SCS) time and the risk of graft failure in DCD liver transplantation.

Six-hour local 4 °C dual hypothermic oxygenated machine perfusion improves the preservation of porcine liver after cardiac death using an ex vivo reperfusion model.

Article	Year
Evaluation of the ex vivo liver viability using a nuclear magnetic resonance relaxation time-based assay in a porcine machine perfusion model. Scientific reports\| PubMed ID: 33603011	2021
Six-hour local 4 °C dual hypothermic oxygenated machine perfusion improves the preservation of porcine liver after cardiac death using an ex vivo reperfusion model. Hepatobiliary & pancreatic diseases international : HBPD INT\| PubMed ID: 40055037	2025

Fen Huo

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