retrospective-observational-study-computed-tomography-based-vascular

Retrospective Observational Study of Computed Tomography-Based Vascular Risk Assessment During Needle Drainage of Peritonsillar Abscess

Peritonsillar abscess (PTA) is a common deep neck infection in which bedside drainage is performed close to major cervical vessels. Although catastrophic ...

investigation-hbsag-anti-hiv-anti-hcv-seropositivity-patients

Investigation of HBsAg, Anti-HIV, and Anti-HCV Seropositivity in Patients Visiting the Orthopedic Clinic

This study aimed to determine the seropositivity of HBsAg, anti-HCV, and anti-HIV among patients admitted to the orthopedics clinic of a medical school ...

Dicle University Faculty of Medicine

Dicle University

Tuberculosis (TB) remains a global health challenge requiring rapid and reliable diagnostic tools. Here, azide-functionalized magnetic nanoparticles (MNPs-N₃) were synthesized, characterized, and applied for one-tube detection of Mycobacterium tuberculosis (Mtb) in sputum. Structural analyses including Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), dynamic light scattering (DLS), and vibrating sample magnetometry (VSM) confirmed successful functionalization, uniform morphology, and preserved superparamagnetism. MNPs-N₃ were integrated into modified Ehrlich-Ziehl-Neelsen (MNPs-N₃-assisted EZN staining) and auramine-rhodamine (MNPs-N₃-assisted AR staining) staining protocols to enhance bacterial capture and visualization without decontamination or centrifugation. Control experiments using non-functionalized magnetic nanoparticles showed no bacterial co-localization, supporting the specificity of the azide-mediated interaction. The entire process was completed within one hour, offering a rapid alternative to conventional culture requiring ≥ 41 days. Using Mycobacterial Growth Indicator Tube (MGIT) culture as the reference, MNPs-N₃-assisted AR staining achieved 99 % sensitivity and 97 % specificity, outperforming MNPs-N₃-assisted EZN staining (95 % and 96 %, respectively). Diagnostic indices, including Youden index (0.96) and F1-score (0.98), demonstrated excellent agreement with culture results. These findings establish MNPs-N₃ as a fast, efficient, and cost-effective tool for Mtb diagnosis. The single-tube workflow minimizes contamination risk and simplifies laboratory handling, supporting potential application in resource-limited settings. Further optimization and large-scale clinical validation are still warranted.

Rapid one-tube sputum processing for tuberculosis diagnosis via azide-functionalized magnetic nanoplatforms with selective bacterial capture.

The prognostic value of blood culture testing in the diagnosis of bacteremia is limited by contamination.

A Multicenter Evaluation of Blood Culture Practices, Contamination Rates, and the Distribution of Causative Bacteria.

Rapid and accurate detection of microorganisms in sterile body fluids, particularly cerebrospinal fluid (CSF), is crucial for effective diagnosis and treatment. Conventional methods, such as centrifugation, may result in low microbial recovery and false negatives, limiting diagnostic accuracy. An alternative, efficient, and accessible microbial concentration method is needed. This study evaluates a nanoparticle-based microbial concentration method to enhance pathogen recovery from CSF. The method was optimized for interaction time (1 min) and nanoparticle dosage (0.01 g/mL) using standard microbial strains, including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Its clinical performance was assessed using 800 CSF samples, and microbial identification was confirmed via MALDI-TOF MS. The method significantly improved microbial recovery across 10-10 CFU/mL concentrations, achieving a detection limit as low as 2 CFU/mL. Clinical validation demonstrated 100% sensitivity and specificity, detecting 15 additional true-positive cases missed by centrifugation. While centrifugation fails to detect bacteria below 10 CFU/mL, our method reliably detects even at 10 CFU/mL, demonstrating superior microbial enrichment, especially in low-biomass samples. This method enhances diagnostic accuracy by reducing false negatives and expediting pathogen detection. Its resource-conscious, low-cost and equipment-free nature makes it particularly beneficial for resource-limited laboratories, offering a scalable alternative for microbial concentration in CSF diagnostics.

ArticleTotal : 2	Year
Publication title	2026
Publication title	2026

Article	Year
Rapid one-tube sputum processing for tuberculosis diagnosis via azide-functionalized magnetic nanoplatforms with selective bacterial capture. Journal of pharmaceutical and biomedical analysis\| PubMed ID: 41475030	2025
A Multicenter Evaluation of Blood Culture Practices, Contamination Rates, and the Distribution of Causative Bacteria. Jundishapur journal of microbiology\| PubMed ID: 27099693	2016
Magnetic nanoparticle-based method for microorganism concentration in sterile body fluids: Validation and clinical applications. World journal of microbiology & biotechnology\| PubMed ID: 40591062	2025

Hakan Temiz

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