mechanism-jianpi-shengxue-decoction-alleviating-cyclophosphamide

Mechanism of Jianpi Shengxue Decoction in Alleviating Cyclophosphamide-Induced Myelosuppression in Mice

The paper aims to investigate how Jianpi Shengxue Decoction (JPSXD) alleviates chemotherapy-induced myelosuppression through network pharmacology and ...

Hunan University of Chinese Medicine

The complexity of natural products always leads to the co-elution of interfering compounds with bioactive compounds, which then has a detrimental effect on structural elucidation. Here, a new method, based on selective solid phase extraction combined with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) spiking and high performance liquid chromatography-diode array detector-quadrupole time-of-flight tandem mass spectrometry (HPLC-DAD-QTOF-MS/MS), is described for sensitive screening, selective extraction and identification of polyphenolic antioxidants in Polygonatum odoratum. First, 25 polyphenolic antioxidants (1-25) were screened by DPPH spiking with HPLC. Second, polydopamine coated Fe3O4 microspheres (Fe3O4@PDA) were prepared to selectively extract target antioxidants with extraction efficiency from 55% to 100% when the amount of Fe3O4@PDA, extraction time, desorption solvent and time were 10mg, 20 min, acetonitrile, and 5 min. Third, 25 antioxidants (10 cinnamides and 15 homoisoflavanones) were identified by HPLC-DAD-QTOF-MS/MS. Furthermore, the DPPH scavenging activities of purified compounds (IC50, 1.6-32.8 μg/mL) validated the method. Among the identified antioxidants, four of them (12, 13, 18 and 19) were new compounds, four of them (2, 4, 8 and 14) were first obtained from family Liliaceae, five of them (1, 3, 5, 7 and 9) were first reported in genus Polygonatum, while one compound (24) was first identified in this species. The results indicated that the proposed method was an efficient and sensitive approach to explore polyphenolic antioxidants from complex natural products.

Sensitive characterization of polyphenolic antioxidants in Polygonatum odoratum by selective solid phase extraction and high performance liquid chromatography-diode array detector-quadrupole time-of-flight tandem mass spectrometry.

In this work, a novel manganese dioxide-graphene nanosheets (MnO-GNSs) composite was synthesized by a facile one-step hydrothermal method, in which manganese dioxide (MnO) was fabricated by hydrothermal reduction of KMnO with GNSs. The structure and morphology of MnO-GNSs composite were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) analysis and X-ray photoelectron spectroscopy (XPS). A sensitive non-enzymatic electrochemical sensor based on MnO-GNSs composite for the detection of low concentration hydrogen peroxide (HO) was fabricated. The electrochemical properties of MnO-GNSs composite modified glassy carbon electrode (MnO-GNSs/GCE) were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and amperometry. The observations confirmed that the fabricated sensor exhibited high electrocatalytic activity for oxidation of HO owing to the catalytic ability of MnO particles and the conductivity of GNSs. Under the optimum conditions, the calibration curve was linear for the amperometric response versus HO concentration over the range 0.5-350 μM with a low detection limit of 0.19 μM (S/N = 3) and high sensitivity of 422.10 μA mM cm. The determination and quantitative analysis of HO in antiseptic solution on MnO-GNSs/GCE exhibited percent recovery of 96.50%-101.22% with relative standard deviation (RSD) of 1.48%-4.47%. The developed MnO-GNSs/GCE might be a promising platform for the practical detection of HO due to its prominent properties including excellent reproducibility, good anti-interference and repeatability.

A sensitive non-enzymatic electrochemical sensor based on acicular manganese dioxide modified graphene nanosheets composite for hydrogen peroxide detection.

Breast cancer (BC) remains the most prevalent malignant tumor in women worldwide and a leading cause of cancer-related mortality. Early screening is essential to improve prognosis, yet current diagnostic methods are often invasive or lack sensitivity. Saliva is an accessible and non-invasive biofluid containing various metabolites that reflect systemic physiological and pathological changes. Thus, salivary metabolomics may provide novel insights into breast cancer-associated metabolic alterations and support the development of early diagnostic strategies.

Article	Year
Sensitive characterization of polyphenolic antioxidants in Polygonatum odoratum by selective solid phase extraction and high performance liquid chromatography-diode array detector-quadrupole... Journal of pharmaceutical and biomedical analysis\| PubMed ID: 25942343	2015
A sensitive non-enzymatic electrochemical sensor based on acicular manganese dioxide modified graphene nanosheets composite for hydrogen peroxide detection. Ecotoxicology and environmental safety\| PubMed ID: 31891837	2020
Identification of potential biomarkers for breast cancer based on salivary metabolomics. Frontiers in oncology\| PubMed ID: 41142631	2025

Xinyu Jiang

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