establishment-evaluation-diabetic-rat-model-with-lower-limb

Establishment and Evaluation of a Diabetic Rat Model with Lower Limb Arteriosclerosis Obliterans

This study aimed to establish and validate a novel rat model of lower extremity arteriosclerosis obliterans (ASO) in diabetic rats. Unlike traditional ...

YanTai Yuhuangding Hospital Affiliated to Qingdao University

Iliac vein compression syndrome (IVCS) can lead to acute deep venous thrombosis (DVT) and post-thrombotic syndrome (PTS). Endovascular venous stenting has become a preferred treatment for IVCS. In this article, we guide stent implantation by the pressure gradient of iliac vein and inferior vena cava. To evaluate the feasibility of guidance of venous stent implantation based on venous pressure gradient difference.

Guidance of Venous Stent Implantation after Catheter-directed Thrombosis in Patients with Acute Left Lower Extremity Deep Venous Thrombosis based on Pressure Gradient Differences between the Iliac Vein and Inferior Vena Cava: A Single-center Retrospective Study.

Infectious aneurysms are rare in clinic with poor therapeutic outcomes. When artery rupture occurs, the disease tends to progress resulting in a high mortality, and there remains no ideal treatment.

In situ reconstruction of ruptured mycotic iliac artery aneurysm with autologous fascial-peritoneal tissue: a case report and literature review.

Stem cell therapy offers a novel approach to treating difbetic foot ulcers. Fish skin decellularized matrix, a type I collagen, provides a promising carrier for stem cells, creating a supportive microenvironment that enhances cell survival and therapeutic potential. This study aims to investigate the effects and mechanisms of human umbilical cord mesenchymal stem cells (HUCMSCs) loaded onto a fish collagen sponge for wound healing in diabetic rats. The study evaluates stem cell-loading efficiency with fish collagen sponge in vitro, assesses material distribution on diabetic rat wounds, and establishes a wound model. Rats are divided into the Self-healing group, Fish collagen sponge group, and Sponge loaded with HUCMSCs group. Therapeutic effects are evaluated through various analyses, including histopathology and reverse transcription polymerase chain reaction for collagen-related gene expression levels. Compared to the self-healing group, both the fish collagen group and the composite group show faster wound repair and improved healing outcomes. The composite group exhibits superior wound healing quality, with fish collagen contributing to enhanced tissue regeneration through collagen regulation at the wound site. Loading HUCMSCs onto a fish collagen sponge shows promise for treating diabetic wounds by addressing nutrient deficiency and cell supply issues, offering potential benefits for patients undergoing treatment.

Fish collagen sponge with human umbilical cord mesenchymal stem cells for diabetic wound repair in rats.

Atherosclerosis, a chronic lipid metabolism disorder, remains a leading cause of morbidity and mortality. While Galnt4, an O-glycosyltransferase, has been implicated in several diseases, its role in atherosclerosis remains poorly understood. This study aimed to elucidate the effects of Galnt4 dysregulation on atherosclerotic lesion formation and its involvement in macrophage lipid metabolism. In this study, we analyzed Galnt4 expression in atherosclerotic plaque tissues and foam cells. In vivo and in vitro experiments were conducted to assess the effects of Galnt4 deficiency on macrophage foam cell formation and plaque development. O-glycoproteomic analysis was performed to explore the mechanistic role of Galnt4 in foam cell formation. Our results revealed that Galnt4 expression was significantly elevated in plaque tissues and foam cells, predominantly localized in macrophages. Galnt4 knockdown reduced macrophage foam cell formation and attenuated plaque development. Mechanistic studies reflected that Galnt4 regulates foam cell formation by modulating lysosomal function, specifically through Lamp-1 glycosylation, leading to decreased lysosomal free cholesterol and reduced foam cell formation. In conclusion, our findings highlighted that Galnt4 in macrophages plays a crucial role in modulating Lamp-1 glycosylation and lysosomal function, thereby impacting foam cell formation and atherosclerosis progression. These findings identify Galnt4 as a potential therapeutic target for atherosclerosis.

Article	Year
Guidance of Venous Stent Implantation after Catheter-directed Thrombosis in Patients with Acute Left Lower Extremity Deep Venous Thrombosis based on Pressure Gradient Differences between the... Annals of vascular surgery\| PubMed ID: 30802569	2019
In situ reconstruction of ruptured mycotic iliac artery aneurysm with autologous fascial-peritoneal tissue: a case report and literature review. BMC surgery\| PubMed ID: 35219293	2022
Fish collagen sponge with human umbilical cord mesenchymal stem cells for diabetic wound repair in rats. Journal of cellular physiology\| PubMed ID: 39429217	2025
Galnt4 Knockdown Modulates Lysosomal Function to Reduce Foam Cell Formation and Attenuate Atherosclerotic Plaque Progression. FASEB journal : official publication of the Federation of American Societies for Experimental Biology\| PubMed ID: 40820898	2025

Haijie Che

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