an-animal-model-pulpitis-based-on-an-outbred-mice-strain-its

An Animal Model of Pulpitis Based on an Outbred Mice Strain and Its Histological Analysis

The Naval Medical Research Institute (NMRI) mice have not been reported previously for creating a dental pulp inflammation model. In this protocol, we ...

Dow University of Health Sciences

Wound healing, being a dynamic process consisting of hemostasis, inflammation, proliferation, and remodeling, involves the complicated interplay of various growth mediators and the cells associated repair system. Current wound healing therapies usually fail to completely regain skin integrity and functionality. Traditionally, curcumin is considered a potent natural wound healing agent as it possesses antibacterial, antioxidant, and anti-inflammatory properties. It is also known that zinc oxide (ZnO) nanoparticles (NPs) have photocatalytic properties, including the generation of reactive oxygen species. ZnO nanoaprticles are also Food and Drug Administration (FDA) approved as safe substances. While ZnO oxide requires illumination with ultraviolet light to become photocatalytically active, dye-sensitized ZnO can be activated by illumination with visible light. In the present study, we explored the wound healing potential of ZnO nanoparticles sensitized with curcumin (Cu+ZnO Nps) and illuminated with visible (blue) light generated by an array of high power LEDs. We studied the antibacterial effect of our conjugates by percentage reduction in bacterial growth and biofilm formation. The wound healing potential was analyzed by percentage wound contraction, biochemical parameters, and histopathological analysis of the wounded site. Additionally, angiogenesis and wound associated cytokines was evaluated by immunohistochemistry of CD31 and gene expression analysis of IL-1β, TNF-α, and MMP-9 after 16 days of post-wound treatment, respectively. Our study suggests that the therapeutic effect of Cu+ZnO NPs with LED illumination increases its wound healing potential by producing an antibacterial and anti-inflammatory effect. Moreover, the treatment strategy of using a nano formulation in combination with LED illumination further increases its efficacy. It was concluded that the anti-inflammatory and bactericidal effects of the LED illuminated Cu+ZnO Np showed accelerated wound healing with increased wound contraction, collagen deposition, angiogenesis, and re-epithelialization.

Investigation of wound healing potential of photo-active curcumin-ZnO-nanoconjugates in excisional wound model.

Wound healing is a multifaceted and complex process that includes inflammation, hemostasis, remodeling, and granulation. Failures in any link may cause the healing process to be delayed. As a result, wound healing has always been a main research focus across the entire medical field, posing significant challenges and financial burdens. Hence, the current investigation focused on the design and development of arginine-modified chitosan/PVA hydrogel-based microneedles (MNs) as a curcumin (CUR) delivery system for improved wound healing and antibacterial activity. The substrate possesses exceptional swelling capabilities that allow tissue fluid from the wound to be absorbed, speeding up wound closure. The antibacterial activity of MNs was investigated against S. aureus and E. coli. The results revealed that the developed CUR-loaded MNs had increased antioxidant activity and sustained drug release behavior. Furthermore, after being loaded in the developed MNs, it revealed improved antibacterial activity of CUR. Wound healing potential was assessed by histopathological analysis and wound closure%. The observed results suggest that the CUR-loaded MNs greatly improved wound healing potential via tissue regeneration and collagen deposition, demonstrating the potential of developed MNs patches to be used as an effective carrier for wound healing in healthcare settings.

Microarray needles comprised of arginine-modified chitosan/PVA hydrogel for enhanced antibacterial and wound healing potential of curcumin.

Thermoresponsive nanoparticles are exploited as drug-delivery vehicles that release their payload upon increment in temperature. We prepared and characterized thermoresponsive lipid-anchored folic acid engineered magnetic nanoparticles (LP-HP-FANPs) that combine receptor-based targeting and thermoresponsive sustained release of hesperidin (HP) in response to endogenous inflammation site temperature. The progressive surface engineering of NPs was validated by FTIR analysis. Our LP-HP-FANPs had a particle size of 100.5 ± 1.76 nm and a zeta potential of 14.6 ± 2.65 mV. The HP encapsulation effectiveness of LP-HP-FANPs is around 91 ± 0.78%. AFM scans indicated that our modified nanoparticles were spherical. LP-HP-FANPs exhibit increased drug release (85.8% at pH 4.0, 50.9% at pH 7.0) at 40 °C. Animal studies showed no toxicity from nanoparticles. Compared to conventional drugs and HP, LP-HP-FANPs effectively decreased paw edema, cytokine levels, and total cell recruitment in thioglycollate-induced peritonitis ( < 0.05). LP-HP-FANPs substantially decreased cytokines compared to HP, HP-FA-NPs, and the standard medication ( < 0.05,  < 0.01, and  < 0.001). These findings imply that the synthesized HP-loaded formulation (LP-HP-FANPs) may be a potential anti-inflammatory formulation for clinical development.

Thermoresponsive lipids engineered magnetic nanoparticles for spatiotemporal delivery of hesperidin to inflammatory sites in animal model.

Severely burned patients require effective regenerative measures to overcome barrier defects and risk of infection. The aim of the investigation was to evaluate the regenerative capacity of DPMSCs (dental pulp-derived mesenchymal stem cells) presented in the form of nanomaterial-enriched scaffolds for tissue regeneration. These injuries specifically need proper wound repair because third and fourth-degree burns cut deep into tissues. Regenerative solutions through mesenchymal stem cells appear very promising for tissue reconstitution. However, tissue engineering has a significant difficulty in improving cell survival and retention in target tissues since direct transplantation cannot achieve these outcomes due to low cell retention rates and cell redistribution into other organs. Therefore, the study focuses on recent advances in developing injectable hydrogels, designed to address these limitations as a three-dimensional cell culturing matrix with increased water absorption capacity . The hydrogel offers an environment that is conducive to cell adhesion and growth, which is not achieved by the stem cells and nanoparticles itself, since the nanoparticles significantly contributes to promote only biological properties. We reported a new nanocomposite hydrogel of chitosan (CHI), poly(-isopropylacrylamide) (PNIPAm) with zinc oxide nanoparticles (ZnO). Physicochemical characterisation demonstrated successful ZnO incorporation, increased structural strength, improved thermal stability, decreased swelling and increased water retention. The  results showed that the isolated cells expressed CD44, CD90 and CD105 positive surface markers of MSCs (mesenchymal stem cells) as well as the capacity of trilineage differentiation. Cytotoxicity studies showed concentration dependent viability for blank hydrogel, whereas, honey-ZnO nanocomposite hydrogel showed significantly high cell survival indicating improved biocompatibility.  tests using a rat scald burn model demonstrated efficient/improved/increased wound healing in the hydrogel treated groups, in particular, when it was associated with dental pulp stem cells (DPMSCs). The analysis of the gene expression profile showed that the treatment with DPMSCs resulted in the significant reduction of several inflammatory cytokines and the elevation of TGF-β (transforming growth factor-β) and COL-1 (collagenase-1), that are possible markers of regeneration. Hypermetabolic response profiling demonstrated that the hydrogels incorporated with DPMSCs, particularly containing honey, were able to suppress systemic hypermetabolism through regulation of corticosterone levels. In summary, the honey-ZnO nanocomposite thermoresponsive hydrogel fusion with DPMSCs has good prospects for deep burn wound treatment due to its enhanced biocompatibility and amplified regeneration properties.

Article	Year
Investigation of wound healing potential of photo-active curcumin-ZnO-nanoconjugates in excisional wound model. Photodiagnosis and photodynamic therapy\| PubMed ID: 35714899	2022
Microarray needles comprised of arginine-modified chitosan/PVA hydrogel for enhanced antibacterial and wound healing potential of curcumin. International journal of biological macromolecules\| PubMed ID: 37673138	2023
Thermoresponsive lipids engineered magnetic nanoparticles for spatiotemporal delivery of hesperidin to inflammatory sites in animal model. Pharmaceutical development and technology\| PubMed ID: 39143894	2024
Evaluating the regenerative capacity of biosynthesized nano formulated 3D scaffolds in scald burn healing. RSC advances\| PubMed ID: 41229970	2025

Shazmeen Aslam

Biography

JoVE Journal Publications

Other Publications