synthesis-r-23-dihydroxypropylphosphonic-acid-via-an-arbuzov-based

Synthesis of (<em>R</em>)-2,3-Dihydroxypropylphosphonic Acid <em>via </em>an Arbuzov-Based Strategy

Phosphonic acids are widely used in medicinal chemistry, chemical biology, and materials research. Their preparation can be difficult. Side reactions are ...

Fuzong Clinical Medical College of Fujian Medical University

 (LM) is one of the most common food-borne pathogens and can induce a series of diseases with a high mortality rate to humans; hence, it is very necessary to develop a highly sensitive method for LM detection. Based on this need, a new sandwich-like electrochemical immunosensing platform was developed herein by preparing carboxyl TiCT MXene (C-TiCT MXene) as the sensing platform and rhodamine b/gold/reduced graphene oxide (RhB/Au/RGO) as the signal amplifier. The high conductivity and large surface area of C-TiCT MXene make it a desirable nanomaterial to fix the primary antibody of LM (PAb), while the prepared Au/RGO/RhB nanohybrid is dedicated to assembling the secondary antibody (SAb) of LM, offering an amplified response signal. Through the use of RhB molecule as the signal probe, the experiments showed that the peak currents of RhB increase along with an increase in the concentration of LM from 10 to 10 CFU mL, and an extremely low limit of detection (2 CFU mL) was obtained on the basis of the proposed immunosensing platform after optimizing various conditions. Hence, it is confirmed that the developed sandwich-like immunosensor based on C-TiCT MXene and RhB/Au/Gr has great application in the detection of LM and other analytes.

A novel electrochemical sandwich-like immunosensor based on carboxyl TiCT MXene and rhodamine b/gold/reduced graphene oxide for .

Exosomes are increasingly recognized as important mediators of intercellular communication in cancer biology. Exosomes can be derived from cancer cells as well as cellular components in tumor microenvironment. After secretion, the exosomes carrying a wide range of bioactive cargos can be ingested by local or distant recipient cells. The released cargos act through a variety of mechanisms to elicit multiple biological effects and impact most if not all hallmarks of cancer. Moreover, owing to their excellent biocompatibility and capability of being easily engineered or modified, exosomes are currently exploited as a promising platform for cancer targeted therapy. In this review, we first summarize the current knowledge of roles of exosomes in risk and etiology, initiation and progression of cancer, as well as their underlying molecular mechanisms. The aptamer-modified exosome as a promising platform for cancer targeted therapy is then briefly introduced. We also discuss the future directions for emerging roles of exosome in tumor biology and perspective of aptamer-modified exosomes in cancer therapy.

Role of Exosomes in Cancer and Aptamer-Modified Exosomes as a Promising Platform for Cancer Targeted Therapy.

Soluble suppression of tumorigenicity 2 (sST2) is a critical biomarker for heart failure (HF) diagnosis and prognosis, yet conventional antibody-based detection methods suffer from time-consuming protocols and high costs and involve complex detection procedures. To address these challenges, we first screened high-affinity aptamers under clinically relevant conditions and then coupled with the CRISPR/Cas12a system to develop a fluorescence aptasensor for rapid and sensitive sST2 detection. A serum matrix was introduced during aptamer selection to enhance specificity and anti-interference performance in real biological environments. Three sST2-specific aptamers (Apt-1, Apt-2, and Apt-3) were identified with dissociation constants () of 8.42, 46.08, and 25.02 nM, respectively. Among these, Apt-1 demonstrated superior performance, which was utilized to construct a fluorescence biosensor combining aptamer recognition with CRISPR/Cas12a trans-cleavage signal amplification. The sensor achieved a broad linear detection range (5-120 ng/mL) and an ultralow limit of detection (LOD, 0.816 ng/mL) when applied in detecting sST2 in both the buffer and human serum. Notably, the platform exhibited exceptional resistance to interference from HF-related proteins and maintained high accuracy in clinical serum samples, showing a strong correlation ( = 0.9794) with enzyme-linked immunosorbent assay (ELISA) results. By integration of serum-matrix screening and CRISPR-based signal enhancement, this work establishes a robust, cost-effective, and rapid diagnostic tool for sST2 detection.

Fluorescence Aptasensor for sST2 Detection Using In Vitro Selected Aptamers.

Heat stroke (HS) is a life-threatening condition marked by hyperthermia, central nervous system dysfunction, and multi-organ injury. Exertional heatstroke (EHS), which commonly affects healthy young people, typically follows intensity training or heavy physical labor in high temperature and high humidity environment. However, the molecular events driving the progression from mild to severe EHS remain poorly understood. This study integrated clinical data with serum proteomics to identify key pathways and biomarkers involved in EHS progression.

Complement-Coagulation Axis and Metabolic Dysregulation Underlie the Transition from Mild to Severe Heat Stroke: A Combined Clinical and Proteomic Study.

Heparin-binding protein (HBP) is a multifunctional cationic protein that is rapidly released upon activation of neutrophils during infectious or inflammatory responses. It plays a crucial role in immune regulation and vascular permeability and has recently emerged as a promising biomarker for the early diagnosis of sepsis. However, conventional antibody-based immunoassays for HBP detection are often expensive, unstable, and labor-intensive, which limits their clinical applicability. Thus, developing sensitive, low-cost, and user-friendly sensing strategies for rapid and accurate HBP quantification is highly desirable.

Ultrasensitive electrochemiluminescence aptasensor for heparin-binding protein based on hybridization chain reaction (HCR) amplification and magnetic enrichment.

Article	Year
A novel electrochemical sandwich-like immunosensor based on carboxyl TiCT MXene and rhodamine b/gold/reduced graphene oxide for . Analytical methods : advancing methods and applications\| PubMed ID: 35156973	2022
Role of Exosomes in Cancer and Aptamer-Modified Exosomes as a Promising Platform for Cancer Targeted Therapy. Biological procedures online\| PubMed ID: 38802766	2024
Fluorescence Aptasensor for sST2 Detection Using In Vitro Selected Aptamers. Analytical chemistry\| PubMed ID: 40370093	2025
Complement-Coagulation Axis and Metabolic Dysregulation Underlie the Transition from Mild to Severe Heat Stroke: A Combined Clinical and Proteomic Study. Shock (Augusta, Ga.)\| PubMed ID: 41587892	2026
Ultrasensitive electrochemiluminescence aptasensor for heparin-binding protein based on hybridization chain reaction (HCR) amplification and magnetic enrichment. Analytica chimica acta\| PubMed ID: 41611406	2026

Shenghang Zhang

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