research-progress-on-the-optimal-timing-for-colostomy-reversal

Research Progress on the Optimal Timing for Colostomy Reversal

This review comprehensively summarizes the latest research progress on the optimal timing for colostomy reversal, a critical surgical intervention aimed ...

The Affiliated Hospital of Southwest Medical University

Colorectal cancer (CRC) is one of the most common diagnosed cancers around the world. The poor prognosis and high fatality caused by metastasis are still the challenges for clinical treatment. Therefore, it is promising to clarify the detailed molecular mechanism of CRC metastasis. Accumulating evidences indicate that long noncoding RNAs (lncRNAs) play important roles in cancer progression including CRC. In this study, the function of lncRNA UCA1 was investigated. UCA1 was confirmed to be highly expressed in colorectal cancer. Moreover, the UCA1 expression level was positively related to tumor stages. Silencing UCA1 showed inhibitory effect on cell proliferation and metastasis. Both UCA1 and NOTCH3 were validated as direct targets of miR-185. Silencing UCA1 repressed NOTCH3 expression through the miR-185 sponge. NOTCH3 was found to be highly expressed in CRC patients and positively related to UCA1 expression. Furthermore, HES5 was verified as a transcription factor of UCA1, which induced UCA1 expression. In conclusion, UCA1 is a direct target of HES5. UCA1 promotes CRC metastasis through regulating the miR-185/NOTCH3 axis.

HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling.

Our previous studies have confirmed that transforming growth factor-β (TGF-β) plays an important role in tumor metastasis, and the serum deprivation protein response (SDPR) is a potential downstream target of TGF-β. However, the role and mechanism of SDPR in gastric cancer are still unclear. We performed gene microarray, bioinformation analysis, combined with  and  experimental verification, we identified that SDPR is significantly downregulated in gastric cancer, and participates in TGF-β-mediated tumour metastasis. Mechanically, SDPR interacts with extracellular signal-regulated kinase (ERK) and inhibits fatty acid metabolism key gene Carnitine palmitoyl transferase 1A (CPT1A) at transcriptional level by supressing ERK/PPAR pathway. Our findings suggest that the TGF-β/SDPR/CPT1A axis play an important role in the fatty acid oxidation of gastric cancer, and provides a new insight into the crosstalk of tumour microenvironments and metabolism reprogramming and suggest that strategies to intervene the fatty acid metabolism may therapy gastric cancer metastasis.

SDPR Inhibits TGF-β Induced Cancer Metastasis Through Fatty Acid Oxidation Regulation in Gastric Cancer.

For cancer patients, red blood cell distribution width (RDW) is a readily accessible and cost-effective preoperative prognostic predictor. This study aimed to determine whether RDW is a predictive factor for individuals undergoing radical surgery for gastric cancer (GC).

The prognostic importance of red blood cell distribution width for gastric cancer: a systematic review and meta-analysis.

Dysregulation of long non‑coding RNAs (lncRNAs) is involved in the development of colorectal cancer (CRC). In the present study, the identification of muscle blind like splicing regulator 1 antisense RNA 1 (MBNL1‑AS1) lncRNA was reported. Firstly, Cell Counting Kit‑8, EdU and colony formation assays were uesed to explore the role of MBNL1‑AS1 in regulating the proliferation of CRC cells. According to TCGA database, it was found that MBNL1‑AS1 was correlated with microRNA (miR)‑29c‑3p and blood vessel epicardial substance (BVES) expression in CRC cells. Then, the regulation among MBNL1‑AS1, miR‑29C‑3P and BVES was detected by dual luciferase reporter assay and the function of MBNL1‑AS1/miR‑29C‑3P/BVES axis was explored by rescue assay. The results demonstrated that MBNL1‑AS1 expression was decreased in CRC and was associated with the size of tumors derived from patients with CRC. Functionally, the upregulation of MBNL1‑AS1 suppressed CRC cell proliferation  and inhibited tumor growth , while knockdown of MBNL1‑AS1 expression caused the opposite effects. MBNL1‑AS1 expression correlated with BVES expression in CRC tissues and MBNL1‑AS1 enhanced the stability of BVES mRNA by functioning as a competing endogenous RNA to sponge miR‑29c‑3p; the latter directly targeted MBNL1‑AS1 and BVES mRNA 3'UTR. Collectively, the results indicated that MBNL1‑AS1 suppressed CRC cell proliferation by regulating miR‑29c‑3p/BVES signaling, suggesting that the MBNL1‑AS1/miR‑29c‑3p/BVES axis may be a potential therapeutic target for CRC.

MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer.

Colorectal cancer (CRC) is a type of intestinal cancer that causes more than 600,000 deaths every year. Overcoming the problems of metastasis requires detailed studies to reveal the potential molecular mechanisms. This study aims to reveal the molecular mechanism of CRC metastasis involving non-coding RNA regulation. The expression profile of FTH1P3 was analyzed based on the data of TCGA-COAD patient cohorts. Q-PCR analysis was performed to validate the expression of FTH1P3 in colorectal cancer cells. JASPR was used to screen transcription factors of FTH1P3. q-ChIP analysis was used to validate the target between FTH1P3 and transcription factor. Scratch assay and transwell assay were used to evaluate the migration and invasion ability of colorectal cancer cells. FTH1P3 is highly expressed in CRC patient cohort. FTH1P3 induced migration and invasion of SW480 cell through regulating epithelial-mesenchymal transition (EMT). In addition, FTH1P3 is a direct target of SNAI2. SNAI2 promotes the expression of FTH1P3. Both FTH1P3 and SNAI2 were directly targeted and repressed by miR-218-5p. Interestingly, ectopic FTH1P3 caused a decreased miR-218-5p level and an elevated nucleic SNAI2 protein expression level. Of note, only ectopic SNAI2 protein resulted in a repressed miR-218-5p and an increased FTH1P3, whereas SNAI2 3'UTR failed to affect the expression of miR-218-5p and FTH1P3. SNAI2 transcriptionally activates FTH1P3 expression. Both SNAI2 and FTH1P3 are targets of miR-218-5p. SNAI2/FTH1P3/miR-218-5p form a positive feedback loop in the regulation of CRC metastasis.

SNAI2/FTH1P3/miR-218-5p Positive Feedback Loop Promotes Colorectal Cancer Metastasis.

The efficacy of single-incision plus one-port laparoscopic surgery (SILS + 1) versus conventional laparoscopic surgery (CLS) for colorectal cancer treatment remains unclear. This study compares the short-term and long-term outcomes of SILS + 1 and CLS using a high-quality systematic review and meta-analysis.

Single-incision plus one-port laparoscopy surgery versus conventional multi-port laparoscopy surgery for colorectal cancer: a systematic review and meta-analysis.

Colorectal cancer (CRC) is a global health burden, highlighting the urgent need for the discovery of new biomarkers and therapeutic targets. This study integrates genetic epidemiology methods, such as Mendelian randomization (MR), with GWAS data to predict treatment efficacy and identify novel CRC therapeutic targets.

Mendelian randomization analysis reveals the potential of the IKZF1 gene as a therapeutic target in colorectal cancer.

Gastric cancer (GC) remains a leading cause of cancer mortality, necessitating robust prognostic biomarkers and personalized therapeutic strategies.

Cell-in-cell associated lncRNA signature predicts prognosis and immunotherapy response in gastric cancer.

With the accelerating global population aging, the proportion of elderly patients with colorectal cancer (CRC) undergoing laparoscopic radical resection is increasing annually. However, significant individual variations in postoperative hospital stay exist, and convenient clinical prediction tools remain lacking. This study aimed to develop and validate a simplified predictive scoring model for postoperative hospital stay in elderly CRC patients after laparoscopic radical resection.

A retrospective study on influencing factors of postoperative hospital stay and development of a predictive scoring model for elderly patients (≥70 years) with colorectal cancer undergoing laparoscopic radical resection.

Anoikis dysregulation and fatty acid metabolism reprogramming are critical for colorectal cancer (CRC) progression, but their integrated role in CRC prognosis remains unclear. We used transcriptomic and clinical data of CRC patients from the cancer genome atlas and gene expression omnibus (GSE12945) databases. Differential analysis, NMF clustering, univariate Cox, and LASSO regression were used to identify (Anoikis-fatty acid metabolism-related gene) AFRGs and construct a prognostic model. The model's performance was validated via receiver operating characteristic curves, nomogram, and immune infiltration analysis. We identified 232 differentially expressed AFRGs and 8 prognostic AFRGs (NMF clustering divided patients into 2 clusters with distinct survival). A 5-gene (BRCA1, CD36, ENO3, INHBB, PHLDA2) model showed robust predictive efficacy (1-, 3-, 5-year OS area under the curve: 0.889/0.795/0.740 in GSE12945). High-risk patients had higher PDCD1/CTLA4 expression and CD8⁺ T cell infiltration. The nomogram (risk score + clinicopathological factors) had high accuracy (area under the curve: 0.788/0.807/0.833). This AFRG-based model is a reliable prognostic tool for CRC and provides insights for personalized treatment. Limitations include retrospective data and small gene expression omnibus cohort, requiring prospective validation.

Construction and validation of a prognostic model based on anoikis-fatty acid metabolism-related genes in colorectal cancer: A retrospective cohort study.

Article	Year
HES5 Activates Long Noncoding RNA UCA1 to Induce Colorectal Cancer Progression by Modulating miR-185/NOTCH3 Signaling. Gastroenterology research and practice\| PubMed ID: 34733326	2021
SDPR Inhibits TGF-β Induced Cancer Metastasis Through Fatty Acid Oxidation Regulation in Gastric Cancer. International journal of biological sciences\| PubMed ID: 37416765	2023
The prognostic importance of red blood cell distribution width for gastric cancer: a systematic review and meta-analysis. Translational cancer research\| PubMed ID: 37588748	2023
MBNL1‑AS1 attenuates tumor cell proliferation by regulating the miR‑29c‑3p/BVES signal in colorectal cancer. Oncology reports\| PubMed ID: 37711058	2023
SNAI2/FTH1P3/miR-218-5p Positive Feedback Loop Promotes Colorectal Cancer Metastasis. Biochemical genetics\| PubMed ID: 37884850	2024
Single-incision plus one-port laparoscopy surgery versus conventional multi-port laparoscopy surgery for colorectal cancer: a systematic review and meta-analysis. International journal of colorectal disease\| PubMed ID: 38684561	2024
Mendelian randomization analysis reveals the potential of the IKZF1 gene as a therapeutic target in colorectal cancer. Discover oncology\| PubMed ID: 40394429	2025
Cell-in-cell associated lncRNA signature predicts prognosis and immunotherapy response in gastric cancer. Frontiers in oncology\| PubMed ID: 40697378	2025
A retrospective study on influencing factors of postoperative hospital stay and development of a predictive scoring model for elderly patients (≥70 years) with colorectal cancer undergoing l... Frontiers in surgery\| PubMed ID: 41394175	2025
Construction and validation of a prognostic model based on anoikis-fatty acid metabolism-related genes in colorectal cancer: A retrospective cohort study. Medicine\| PubMed ID: 41496025	2026

Zhengfei Zhao

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