a-surgical-method-for-subvalvular-structure-intervention-through

A Surgical Method for Subvalvular Structure Intervention Through a Transaortic Approach in Normal Bama Pigs

Subvalvular structure abnormalities leading to left ventricular outflow tract (LVOT) alterations and myocardial hypertrophy are characteristic anatomical ...

Chinese Academy of Medical Sciences and Peking Union Medical College

 is a microaerophilic Gram-negative bacterium that resides in the human stomach and is classified as a class I carcinogen for gastric cancer. Numerous studies have demonstrated that  infection plays a role in regulating the function of host cells, thereby contributing to the malignant transformation of these cells. However,  infection is a chronic process, and short-term cellular experiments may not provide a comprehensive understanding of the  situation, especially when considering the lower oxygen levels in the human stomach. In this study, we aimed to investigate the mechanisms underlying gastric cell dysfunction after prolonged exposure to  under hypoxic conditions. We conducted a co-culture experiment using the gastric cell line GES-1 and  for 30 generations under intermittent hypoxic conditions. By closely monitoring cell proliferation, migration, invasion, autophagy, and apoptosis, we revealed that sustained  stimulation under hypoxic conditions significantly influences the function of GES-1 cells. This stimulation induces epithelial-mesenchymal transition and contributes to the propensity for malignant transformation of gastric cells. To confirm the  results, we conducted an experiment involving Mongolian gerbils infected with  for 85 weeks. All the results strongly suggest that the Nod1 receptor signaling pathway plays a crucial role in -related apoptosis and autophagy. In summary, continuous stimulation by  affects the functioning of gastric cells through the Nod1 receptor signaling pathway, increasing the likelihood of cell carcinogenesis. The presence of hypoxic conditions further exacerbates this process.IMPORTANCEDeciphering the collaborative effects of  infection on gastric epithelial cell function is key to unraveling the development mechanisms of gastric cancer. Prior research has solely examined the outcomes of short-term  stimulation on gastric epithelial cells under aerobic conditions, neglecting the bacterium's nature as a microaerophilic organism that leads to cancer following prolonged stomach colonization. This study mimics a more genuine  infection scenario by repeatedly exposing gastric epithelial cells to  under hypoxic conditions for up to 30 generations. The results show that chronic exposure to  in hypoxia substantially increases cell migration, invasion, and epithelial-mesenchymal transition, while suppressing autophagy and apoptosis. This highlights the significance of hypoxic conditions in intensifying the carcinogenic impact of  infection. By accurately replicating the  gastric environment, this study enhances our comprehension of 's pathogenic mechanisms in gastric cancer.

Hypoxia exacerbates the malignant transformation of gastric epithelial cells induced by long-term  infection.

Organ failure poses a substantial global health challenge, and xenotransplantation emerges as one of the most promising avenues to mitigate the critical shortage of donor organs. In recent years, numerous research institutions have undertaken clinical and preclinical xenotransplantation in humans, instilling hope for notable progress. Nevertheless, formidable obstacles persist before success can be fully achieved. Chinese researchers have been at the forefront of xenotransplantation studies, actively contributing to several pivotal areas: the identification of critical genes essential for xenotransplantation and the creation of genetically modified pigs; preclinical studies on pig-to-nonhuman primate organ and tissue xenotransplantation, as well as the utilization of genetically engineered pig-derived biomaterials; contributions to both preclinical and clinical xenotransplantation research; and the formulation and refinement of xenotransplantation policies and ethical guidelines in China. In conclusion, this review seeks to not only acknowledge the contributions of Chinese researchers but also to encourage further collaboration between Chinese scholars and their international counterparts in advancing the field of xenotransplantation.

Xenotransplantation in China: Past, Present, and Future.

Arrhythmogenic cardiomyopathy (ACM) is an inherited disorder involving ventricular arrhythmias, cardiac dysfunction, and fibrofatty myocardial replacement. Current treatments are largely palliative, with heart transplantation as the only definitive option for advanced ACM. Here, we show that, building upon our previous identification of a patient with a PNPLA2 mutation, we developed a murine model carrying the same mutation, faithfully mimicking key ACM phenotypes such as arrhythmias, lipid accumulation, and fibrosis. Using an adeno-associated virus 9 (AAV9) vector to deliver the human PNPLA2 gene, we demonstrated that early intervention prevented ACM onset, while later treatment reversed established symptoms and extended survival. Treated mice exhibited improved cardiac function, lipid metabolism, and normalized fatty acid pathways, verified by single-nucleus sequencing. These findings highlight the promise of AAV9-mediated PNPLA2 gene supplementation as an effective therapeutic strategy for ACM, supporting further clinical exploration.

Article	Year
Hypoxia exacerbates the malignant transformation of gastric epithelial cells induced by long-term infection. Microbiology spectrum\| PubMed ID: 38916312	2024
Xenotransplantation in China: Past, Present, and Future. Xenotransplantation\| PubMed ID: 40243324	2025
AAV9-mediated gene supplementation therapy prevents and rescues arrhythmogenic cardiomyopathy in Pnpla2-mutated mice. Molecular therapy : the journal of the American Society of Gene Therapy\| PubMed ID: 40518669	2025

Xiulin Zhang

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