a-comprehensive-workflow-for-plasma-proteomics-mass-spectrometry

A Comprehensive Workflow for Plasma Proteomics by Mass Spectrometry: A Strategy for Biomarker Discovery in Systemic Human Diseases

Plasma, the cell-free component of blood, offers a minimally invasive and rich source of biomolecular information, making it ideal for investigating ...

Jubilee Mission Medical College and Research Institute

Neuromyelitis optica spectrum disorders (NMOSD) is a demyelinating autoimmune disease affecting the central nervous system causing inflammatory lesions in the optic nerves, spinal cord and other vital areas of CNS. The clinical manifestations include acute transverse myelitis with paraplegia and optic neuritis with impaired vision. In the present study we focussed on comparative expression of serum proteins between NMOSD variants and control. The study has identified a plethora of novel and unexplored acute phase proteins involved in lipid transport and myelination in perspective of NMOSD. The serum proteins obtained after albumin globulin depletion were subjected to LC-MS/MS. The commonly altered proteins in all NMOSD variants with respect to control were smaug homolog protein and serum amyloid A. Truncated breast and ovarian cancer susceptibility protein and cystic fibrosis transmembrane conductance regulator protein were specifically upregulated and can act as potential biomarkers for neuromyelitis optica with autoantibody negativity to Aquaporin - 4 (AQP-4) and myelin oligodendrocyte (MOG) immunoglobulins. In addition, the uniquely downregulated proteins such as antithrombin III and histidine rich glycoprotein in NMO/MOG autoantibody negative samples can be accounted for its dysregulated fibrinolysis associated with NMO. The differentially expressed proteins were involved in cholesterol transport, synaptic vesicle mediated transport, neurotransmission and immune regulation which are closely associated with myelin formation and protection

Identification of Novel Serum Proteins Associated with Myelination and Cholesterol Transport in Neuromyelitis Optica Spectrum Disorders by Mass Spectrometry.

COPD is a complex respiratory disorder with high morbidity and mortality rates. Even with the current conventional diagnostic methods, including circulating inflammatory biomarkers, underdiagnosis rates in COPD remain as high as 70%. Our study was a comparative cross-sectional study that aimed to address the diagnostic challenges by identifying future biomarker candidates in COPD variants.

Exploring the novel duo of Reticulocalbin, and Sideroflexin as future biomarker candidates for Exacerbated Chronic Obstructive Pulmonary Disease.

Current diagnostic methods for JIA lack specificity, often failing to distinguish it from other childhood diseases of similar clinical presentations. The present study is a comparative cross-sectional study that identified potential biomarkers using label-free mass spectrometry and bioinformatics. Two differentially expressed proteins (DEPs), Myosin light chain 12b (Myl12b) and Mannose-binding lectin serine protease 1 (MASP1), showed increased expression in JIA patients. Receiver operating characteristic (ROC) analysis revealed strong predictive power (AUC: Myl12b = 0.757, MASP1 = 0.855), validated in a separate cohort via Western blot and ELISA. These findings suggest Myl12b and MASP1 as promising biomarkers for JIA diagnosis and treatment. Data: ProteomeXchange (PXD058863).

Myosin Light Chain 12b and MASP1 as Novel Biomarker Candidates in Active Juvenile Idiopathic Arthritis─A Combined Proteomics/Bioinformatics Approach.

Inflammation is crucial for wound healing, but chronic inflammation leads to complications.  L. leaves possess anti-inflammatory properties, and this study explores the effects of the Flavonoid Fraction of  (FFCP) leaf on inflammation and wound healing. In human PBMCs, the FFCP treatment efficiently reduced the activity of COX, LOX, iNOS, NO productions, and the release of proinflammatory cytokines, including TNF-α, IL-1β, and IL-6 into the culture media. Molecular docking revealed that glycosidic flavonoids were more potent COX-2 and 5-LOX inhibitors than aglycones. FFCP (100 μg/mL) significantly enhanced cell migration, closing the wound within 36 h. These findings suggest that FFCP possesses anti-inflammatory properties and promote fibroblast migration , highlighting the potential therapeutic application of  flavonoids in inflammation management and tissue repair.

Article	Year
Identification of Novel Serum Proteins Associated with Myelination and Cholesterol Transport in Neuromyelitis Optica Spectrum Disorders by Mass Spectrometry. Indian journal of clinical biochemistry : IJCB\| PubMed ID: 35873620	2022
Exploring the novel duo of Reticulocalbin, and Sideroflexin as future biomarker candidates for Exacerbated Chronic Obstructive Pulmonary Disease. Clinical proteomics\| PubMed ID: 38355435	2024
Myosin Light Chain 12b and MASP1 as Novel Biomarker Candidates in Active Juvenile Idiopathic Arthritis─A Combined Proteomics/Bioinformatics Approach. Journal of proteome research\| PubMed ID: 39932158	2025
Modulation of COX-2, 5-LOX, and cytokine signalling by L. Leaf cultivar 'Red Lady' flavonoids in inflammation: and insights. Natural product research\| PubMed ID: 41022640	2025

Evelyn Maria

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