development-efficacy-enzyme-responsive-squalene-chidamide

Development and Efficacy of Enzyme-Responsive Squalene-Chidamide Nanoparticles for Pancreatic Cancer

Here, we present a protocol to address the limited intratumoral penetration of small-molecule drugs caused by the dense extracellular matrix (ECM) of ...

Zhejiang Chinese Medical University

Previous studies have revealed the close relation of irisin with the occurrence and development of nonalcoholic fatty liver disease (NAFLD). A systematic review and meta-analysis were conducted to evaluate the association of circulating irisin levels and NAFLD.

Circulating Irisin Levels in Patients with Nonalcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis.

Radix Paeoniae Alba is a traditional Chinese herbal medicine. It can accelerate salivary secretion and alleviate the dry mouth of patients with Sjogren's syndrome (SS). Although it is widely used in clinical treatment, its target and mechanism remain unclear.

Exploring the Target and Mechanism of Radix Paeoniae Alba on Sjogren's Syndrome.

Hepatocellular carcinoma (HCC) is a common malignancy with poor prognosis and high mortality. This study aimed to explore the oncogenic mechanisms of TRAF5 in HCC and provide a novel therapeutic strategy for HCC.

Silencing of TRAF5 enhances necroptosis in hepatocellular carcinoma by inhibiting LTBR-mediated NF-κB signaling.

Primary Sjogren's syndrome (pSS) is a slowly progressive, systemic autoimmune disorder characterized by gradual lymphocytic infiltration of exocrine glands. However, the spatially profiling the immune microenvironment in pSS is largely unclear, limiting the understanding of the complex interplay among cells within the microenvironment. Based on imaging mass cytometry (IMC) analysis of clinical pSS samples, we first revealed that labial salivary gland (LSG) comprised of epithelial, immune cells and stromal cells, and epithelial was the main cell type in LSG. Eight immune cells populations were identified, including CD8 T, CD4 T, Treg, B, NK cells, neutrophils, resident macrophages and a mixed immune cell cluster. We found that CD8 T cells, but not CD4 T cells, were the most prominent T cells in immune infiltrates of pSS LSG. With the increase of pSS disease activity and severity, the infiltration abundance of CD8 T cells gradually increased and was accompanied by the activation of inflammatory response. sc-RNA-seq analysis based on the GSE272409 dataset confirmed that CD8 T cells were the main immune cells, and dominated the most intercellular ligand-receptor interactions. CD8 T cells were further clustered into five cell subsets, of which CD160CD8 T cells subset appeared to present only in pSS patients. Further experiments demonstrated that CD160 expression on CD8 T cells was associated with an enhanced expression of proinflammatory and cytotoxic cytokines IFN-γ, GZMB and TNF-α, and the injury of salivary gland epithelial cells. Besides, proportion of GZMKCD8 T cells subset was increased in pSS patients. Trajectory analysis confirmed an enhanced frequency of CD8 T cell differentiation and activation during the progression of pSS. This study provided single cell profile with spatial information for analyzing the LSG immune microenvironment in pSS, which could not be achieved by conventional immunofluorescence and immunohistochemistry assays.

Application of imaging mass cytometry for spatially profiling the microenvironment of salivary glands in primary Sjögren's syndrome.

The role of MDM Two Binding Protein (MTBP) in tumor regulation is controversial; METHODS: We gathered MTBP's genomic location and GTEx expression data from University of California Santa Cruz (UCSC) and utilized the Human Protein Atlas (HPA) to evaluate its expression. Survival prognosis and immune infiltration analysis were meticulously evaluated by downloading and processing TCGA RNAseq data. Genetic alterations in MTBP were explored using cBioPortal; (3) Our findings revealed a prevailing trend of MTBP overexpression in numerous cancer types, associating with adverse prognoses and implicating MTBP as an oncogenic facilitator. Notably, in certain cancers, reduced MTBP expression correlated with improved patient survival, reflecting a tumor suppressive function of MTBP. By analyzing the tumor immunological environment, we observed positive correlations between MTBP and specific T helper cell subsets, suggesting its potential role in immune evasion strategies. Phosphorylation emerged as a pivotal post-translational modification regulating MTBP's function, influencing DNA replication process frequently exploited by cancer cells. Functional enrichment analyses highlighted MTBP's involvement in nuclear division, organelle fission, and cell cycle regulation, reinforcing its centrality in tumor proliferation and differentiation; CONCLUSIONS: Our results position MTBP as a critical regulator of cancer progression, with implications for immune modulation and DNA replication control.

Unveiling the enigmatic role of MTBP in pan-cancer: A bioinformatics perspective.

S100 protein family members S100A8 and S100A9 function primarily as a heterodimer complex (S100A8/A9) in vivo. This complex has been implicated in various cancers, including gastric cancer (GC). Recent studies suggest that these proteins play significant roles in tumor progression, inflammation, and metastasis. However, the exact mechanisms by which S100A8/A9 contributes to GC pathogenesis remain unclear. This study investigates the role of S100A8/A9 and its receptor in GC. Immunohistochemical analysis was performed on GC tissue samples to assess the expression of the S100A8/A9 receptor melanoma cell adhesion molecule (MCAM). In vitro transwell migration and invasion assays were used to evaluate the motility and invasiveness of GC cells. Cell proliferation was assessed using a growth assay, and Western blotting (WB) was employed to examine downstream signaling pathways, including ERK and the transcription factor c-Jun, in response to S100A8/A9-MCAM interaction. S100A8/A9 stimulation enhanced both proliferation and migration through MCAM binding in GC cell lines. These cellular events were accompanied by ERK activation and c-Jun induction. Downregulation of MCAM suppressed both ERK phosphorylation and c-Jun expression, highlighting the importance of the S100A8/A9‒MCAM‒ERK‒c-Jun axis in promoting GC progression. These findings indicate that S100A8/A9 contributes to GC progression via MCAM, which activates the ERK‒c-Jun pathway. The S100A8/A9‒signaling axis may represent a novel therapeutic target in GC.

S100A8/A9-MCAM signaling promotes gastric cancer cell progression via ERK-c-Jun activation.

Mechanism-driven innovative drug development for primary Sjögren's disease: insights from global clinical trial landscapes.

To investigate the effect of the Yangyin Yiqi Huoxue formula on the oral microecology in a mouse model of Sjögren's syndrome (SS), and to explore the underlying mechanisms.

Article	Year
Circulating Irisin Levels in Patients with Nonalcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis. Gastroenterology research and practice\| PubMed ID: 33224193	2020
Exploring the Target and Mechanism of Radix Paeoniae Alba on Sjogren's Syndrome. Combinatorial chemistry & high throughput screening\| PubMed ID: 36017844	2023
Silencing of TRAF5 enhances necroptosis in hepatocellular carcinoma by inhibiting LTBR-mediated NF-κB signaling. PeerJ\| PubMed ID: 37366426	2023
Application of imaging mass cytometry for spatially profiling the microenvironment of salivary glands in primary Sjögren's syndrome. Cell death & disease\| PubMed ID: 40379615	2025
Unveiling the enigmatic role of MTBP in pan-cancer: A bioinformatics perspective. Pathology, research and practice\| PubMed ID: 40680629	2025
S100A8/A9-MCAM signaling promotes gastric cancer cell progression via ERK-c-Jun activation. In vitro cellular & developmental biology. Animal\| PubMed ID: 40924339	2025
Mechanism-driven innovative drug development for primary Sjögren's disease: insights from global clinical trial landscapes. Clinical and experimental rheumatology\| PubMed ID: 41410588	2025
[Effects and Mechanisms of Yangyin Yiqi Huoxue Formula on the Oral Microecology in Sjögren's Syndrome Model Mice]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition\| PubMed ID: 41536683	2025

Fangping Wu

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