purification-hla-g-extravillous-trophoblasts-from-human-term

Purification of HLA-G<sup>+</sup> Extravillous Trophoblasts from Human Term Placental Tissues for Phenotyping and Functional Analysis

The human maternal-fetal interface represents a unique immunological environment where maternal immune cells and fetal extravilllous trophoblasts (EVT) ...

Cincinnati Children's Hospital Medical Center

University of Cincinnati College of Medicine

Decidual natural killer cells (dNKs) are key immune effector cells at the maternal-fetal interface. dNKs utilize the antimicrobial peptide granulysin (GNLY) to kill bacteria without killing infected trophoblasts. Since rodents lack the GNLY gene, the in vivo biology of GNLY expression by dNKs and their role during intra-uterine infection (IUI) is not well understood. Here, we defined dNK GNLY expression in placental membranes in a rhesus macaque model of IUI and compared it with human acute chorioamnionitis (ACA). GNLY-high dNKs of both species shared conserved transcriptional and protein signatures of antimicrobial defense and immunoregulation. Moreover, GNLY-high dNKs responded to inflammation and infection by increasing their cytotoxic, cytokine, and inflammatory signatures. Thus, high GNLY expression defines a unique dNK type with active roles in immune defense. Defining the regulatory networks of GNLY expression by dNKs and their unique mechanisms of infection control will generate therapeutic opportunities to enhance immunity and reduce IUI-related pregnancy complications.

Angel Dasenbrock

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