phosphorus-31-magnetic-resonance-spectroscopy-for-serial-measurements

Phosphorus-31 Magnetic Resonance Spectroscopy for Serial Measurements of Cardiac Energetics During Temperature-Controlled Heart Preservation

Reliable organ preservation is critical to increasing donor availability and improving transplant outcomes. While static storage remains the standard for ...

Johns Hopkins University

Molecular mismatch analysis is increasingly used to assess donor-recipient compatibility in transplantation. We sought to determine if reactivity against continuous HLA-DR epitopes could be directly measured using a peptide-based immunoassay. We used sequence and informatics analysis to identify putative short linear epitopes in two polymorphic regions surrounding amino acid (aa) position 30 and 70 of HLA-DRB1*07:01 and HLA-DRB1*08:01, respectively. We tested sera from patients who were HLA antibody negative, HLA class II antibody positive for irrelevant antigens, or positive for the HLA-DR7 or -DR8 antigen by Single Antigen Bead testing. Among HLA-DR7 reactive sera, we found that 9/18 (50%) were above a positive threshold for one or both peptide epitopes, while 7/19 (36%) sera were positive for at least one HLA-DR8 peptide epitopes. This proof-of-concept study demonstrates that short peptide targets can be used to identify HLA antibody reactivity, which could be used to directly interrogate HLA epitope-level humoral alloimmunity.

Dhruvi Mehta

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