robot-assisted-laparoscopic-splenectomy-children-case-report-with

Robot-Assisted Laparoscopic Splenectomy In Children: A Case Report with Literature Review

In March 2025, a pediatric patient diagnosed with hereditary spherocytosis (HS) was admitted to the Department of Gastrointestinal Surgery at Xiangyang ...

Wuhan University of Science and Technology

Vacuum sealing drainage (VSD) is frequently used in abdominal surgeries. However, relevant guidelines are rare. Chinese Trauma Surgeon Association organized a committee composed of 28 experts across China in July 2017, aiming to provide an evidence-based recommendation for the application of VSD in abdominal surgeries. Eleven questions regarding the use of VSD in abdominal surgeries were addressed: (1) which type of materials should be respectively chosen for the intraperitoneal cavity, retroperitoneal cavity and superficial incisions? (2) Can VSD be preventively used for a high-risk abdominal incision with primary suture? (3) Can VSD be used in severely contaminated/infected abdominal surgical sites? (4) Can VSD be used for temporary abdominal cavity closure under some special conditions such as severe abdominal trauma, infection, liver transplantation and intra-abdominal volume increment in abdominal compartment syndrome? (5) Can VSD be used in abdominal organ inflammation, injury, or postoperative drainage? (6) Can VSD be used in the treatment of intestinal fistula and pancreatic fistula? (7) Can VSD be used in the treatment of intra-abdominal and extra-peritoneal abscess? (8) Can VSD be used in the treatment of abdominal wall wounds, wound cavity, and defects? (9) Does VSD increase the risk of bleeding? (10) Does VSD increase the risk of intestinal wall injury? (11) Does VSD increase the risk of peritoneal adhesion? Focusing on these questions, evidence-based recommendations were given accordingly. VSD was strongly recommended regarding the questions 2-4. Weak recommendations were made regarding questions 1 and 5-11. Proper use of VSD in abdominal surgeries can lower the risk of infection in abdominal incisions with primary suture, treat severely contaminated/infected surgical sites and facilitate temporary abdominal cavity closure.

Chinese Trauma Surgeon Association for management guidelines of vacuum sealing drainage application in abdominal surgeries-Update and systematic review.

An epidemic of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spreading worldwide. SARS-CoV-2 relies on its spike protein to invade host cells by interacting with the human receptor protein Angiotensin-Converting Enzymes 2 (ACE2). Therefore, designing an antibody or small-molecular entry blockers is of great significance for virus prevention and treatment. This study identified five potential small molecular anti-virus blockers via targeting SARS-CoV-2 spike protein by combining in silico technologies with in vitro experimental methods. The five molecules were natural products that binding to the RBD domain of SARS-CoV-2 was qualitatively and quantitively validated by both native Mass Spectrometry (MS) and Surface Plasmon Resonance (SPR). Anti-viral activity assays showed that the optimal molecule, H69C2, had a strong binding affinity (dissociation constant K) of 0.0947 µM and anti-virus IC of 85.75 µM.

Discovery of potential small molecular SARS-CoV-2 entry blockers targeting the spike protein.

The global COVID-19 coronavirus pandemic has infected over 109 million people, leading to over 2 million deaths up to date and still lacking of effective drugs for patient treatment. Here, we screened about 1.8 million small molecules against the main protease (M) and papain like protease (PL), two major proteases in severe acute respiratory syndrome-coronavirus 2 genome, and identified 1851M inhibitors and 205 PL inhibitors with low nmol/l activity of the best hits. Among these inhibitors, eight small molecules showed dual inhibition effects on both M and PL, exhibiting potential as better candidates for COVID-19 treatment. The best inhibitors of each protease were tested in antiviral assay, with over 40% of M inhibitors and over 20% of PL inhibitors showing high potency in viral inhibition with low cytotoxicity. The X-ray crystal structure of SARS-CoV-2 M in complex with its potent inhibitor 4a was determined at 1.8 Å resolution. Together with docking assays, our results provide a comprehensive resource for future research on anti-SARS-CoV-2 drug development.

High-throughput screening of SARS-CoV-2 main and papain-like protease inhibitors.

Microcystin-LR (MCLR) poses a significant threat to aquatic ecosystems and public health. This study investigated the protective effects of the probiotic  against MCLR-induced developmental toxicity in zebrafish larvae. Zebrafish larvae were exposed to various concentrations of MCLR (0, 0.9, 1.8, and 3.6 mg/L) with or without  from 72 to 168 h post-fertilization (hpf). Probiotic supplementation significantly improved survival, hatching, and growth rates and reduced malformation rates in MCLR-exposed larvae.  alleviated MCLR-induced oxidative stress by reducing reactive oxygen species (ROS) levels and enhancing glutathione (GSH) content and catalase (CAT) activity. Probiotics also mitigated MCLR-induced lipid metabolism disorders by regulating key metabolites (triglycerides, cholesterol, bile acids, and free fatty acids) and gene expression (, , , and ). Moreover, 16S rRNA sequencing revealed that  modulated the gut microbiome structure and diversity in MCLR-exposed larvae, promoting beneficial genera like  and  and inhibiting potential pathogens like . Significant correlations were found between gut microbiota composition and host antioxidant and lipid metabolism parameters. These findings suggest that  exerts protective effects against MCLR toxicity in zebrafish larvae by alleviating oxidative stress, regulating lipid metabolism, and modulating the gut microbiome, providing insights into probiotic-based strategies for mitigating MCLR toxicity in aquatic organisms.

Probiotics Alleviate Microcystin-LR-Induced Developmental Toxicity in Zebrafish Larvae.

This study was aimed at developing a systematic and standardized occupational protection theory to guide new medical staff in protection against occupational exposure.

Construction of a Two-Way and Progressive Occupational Protection Model for New Medical Staff: A Grounded Theory Study.

The dietary inflammatory index (DII) is a literature-derived index to assess the inflammatory potential of diet. While numerous studies have linked a higher DII score to an increased risk of mortality, there are limited studies among individuals with diabetes.

Article	Year
Chinese Trauma Surgeon Association for management guidelines of vacuum sealing drainage application in abdominal surgeries-Update and systematic review. Chinese journal of traumatology = Zhonghua chuang shang za zhi\| PubMed ID: 30850324	2019
Discovery of potential small molecular SARS-CoV-2 entry blockers targeting the spike protein. Acta pharmacologica Sinica\| PubMed ID: 34349236	2022
High-throughput screening of SARS-CoV-2 main and papain-like protease inhibitors. Protein & cell\| PubMed ID: 36726755	2023
Probiotics Alleviate Microcystin-LR-Induced Developmental Toxicity in Zebrafish Larvae. Toxics\| PubMed ID: 39058179	2024
Construction of a Two-Way and Progressive Occupational Protection Model for New Medical Staff: A Grounded Theory Study. Journal of multidisciplinary healthcare\| PubMed ID: 40401078	2025
Diabetes duration-specific association of dietary inflammatory index with the risk of mortality among individuals with diabetes. Diabetology & metabolic syndrome\| PubMed ID: 40551226	2025

Ya Zhu

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