a-simplified-robust-protocol-for-isolation-long-term-expansion

A Simplified and Robust Protocol for the Isolation and Long-term Expansion of Primary Human Endometrial Epithelial Cells

Research on endometrial disorders, including tamoxifen-associated endometrial hyperplasia, has been limited by the lack of robust in vitro epithelial ...

The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine)

Drug resistance in tumors is one of the major factors that leads to chemotherapy failure. This study aims to investigate the effect of Radix Tetrastigma extracts (RTEs) on Taxol-induced autophagy and the chemosensitivity against drug resistance in triple-negative breast cancer (TNBC).

Radix Tetrastigma Extracts Enhance the Chemosensitivity in Triple-Negative Breast Cancer Via Inhibiting PI3K/Akt/mTOR-Mediated Autophagy.

Immunotherapy has made a breakthrough in triple negative breast cancer (TNBC). The aim of this study is to investigate the specific role and regulatory mechanism of 3-oxoacid CoA-transferase 1 (OXCT1) in influencing TNBC growth and immune escape induced by aerobic glycolysis. OXCT1-induced enhancement of TNBC cell proliferation and PD-L1 expression is reversed by 2-DG. After interference with OXCT1 in TNBC patient-derived organoids (PDOs), tumor cell proliferation and lactic acid secretion are attenuated, and T-cell killing is enhanced. OXCT1 correlates with phosphoglycerate kinase 1 (PGK1) protein expression in clinical TNBC samples. In vitro overexpression of OXCT1 has no significant effect on PGK1 mRNA expression, but increases the succinylation level of PGK1 K146 and its protein stability, while decreasing its ubiquitination. The H4K20me1 level in the OXCT1 promoter region is increased in TNBC tissues, and in vitro lysine methyltransferase 5 A (KMT5A) overexpression increases the H4K20me1 level in the OXCT1 promoter region to promote OXCT1 expression. In conclusion, OXCT1 interference reverses the KMT5A-induced enhancement of TNBC cell viability, proliferation, and PD-L1 expression. KMT5A promotes OXCT1 expression through histone methylation, and OXCT1 increases PGK1 protein stability through succinylation modification, thereby promoting aerobic glycolysis and immune escape in TNBC.

OXCT1 promotes triple negative breast cancer immune escape via modulating succinylation modification of PGK1.

Breast cancer is the most common malignant tumor among women globally, with incidence and mortality rates ranking first among female cancers. Mitochondrial dysfunction plays a crucial role in the occurrence, development, and metastasis of breast cancer, but the specific mechanisms remain unclear. This study aims to explore the mechanisms and prognostic value of the mitochondrial-related gene PARK7 in breast cancer.

Article	Year
Radix Tetrastigma Extracts Enhance the Chemosensitivity in Triple-Negative Breast Cancer Via Inhibiting PI3K/Akt/mTOR-Mediated Autophagy. Clinical breast cancer\| PubMed ID: 34535390	2022
OXCT1 promotes triple negative breast cancer immune escape via modulating succinylation modification of PGK1. Communications biology\| PubMed ID: 40634657	2025
The role and prognostic value of mitochondria-related gene PARK7 in breast cancer. Breast cancer research : BCR\| PubMed ID: 41219819	2025

Xiaoai Lv

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