preparation-economical-universal-compensation-beads-compatible-for

Preparation of Economical and Universal Compensation Beads Compatible for Multi-species Antibodies

This protocol describes a cost-effective and reproducible method for preparing multi-species-compatible compensation beads for fluorescence compensation ...

The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital

To explore the feasibility of differentiating three predominant metastatic tumor types using lung computed tomography (CT) radiomics features based on supervised machine learning.

Differentiation of Lung Metastases Originated From Different Primary Tumors Using Radiomics Features Based on CT Imaging.

To build CT perfusion (CTP)-based delta-radiomics models to identify collateral vessel formation after revascularization in patients with moyamoya disease (MMD).

CT perfusion-based delta-radiomics models to identify collateral vessel formation after revascularization in patients with moyamoya disease.

To develop and validate a clinical-radiomics nomogram based on radiomics features and clinical risk factors for identification of human epidermal growth factor receptor 2 (HER2) status in patients with breast cancer (BC).

Clinical-radiomics nomogram for identifying HER2 status in patients with breast cancer: A multicenter study.

Astrocyte elevated gene-1 (AEG-1) is an important oncogene that overexpresses in gliomas and plays a vital role in their occurrence and progression. However, few reports have shown which biomarkers could reflect the level of AEG-1 expression in vivo so far. In recent years, intracellular metabolites monitored by proton magnetic resonance spectroscopy (1H MRS) as non-invasive imaging biomarkers have been applied to the precise diagnosis and therapy feedback of gliomas. Therefore, understanding the correlation between 1H MRS metabolites and AEG-1 gene expression in U251 cells may help to identify relevant biomarkers. This study constructed three monoclonal AEG-1-knockout U251 cell lines using the clustered regularly interspaced short palindromic repeat (CRISPR) /Cas9 technique and evaluated the biological behaviors and metabolite ratios of these cell lines. With the decline in AEG-1 expression, the apoptosis rate of the AEG-1-knockout cell lines increased. At the same time, the metastatic capacities decreased, and the relative contents of total choline (tCho) and lactate (Lac) were also reduced. In conclusion, deviations in AEG-1 expression influence the apoptosis rate and metastasis capacity of U251 cells, which the 1H MRS metabolite ratio could monitor. The tCho/creatinine(Cr) and Lac/Cr ratios positively correlated with the AEG-1 expression and malignant cell behavior. This study may provide potential biomarkers for accurate preoperative diagnosis and future AEG-1-targeting treatment evaluation of gliomas in vivo.

Article	Year
Differentiation of Lung Metastases Originated From Different Primary Tumors Using Radiomics Features Based on CT Imaging. Academic radiology\| PubMed ID: 35577699	2023
CT perfusion-based delta-radiomics models to identify collateral vessel formation after revascularization in patients with moyamoya disease. Frontiers in neuroscience\| PubMed ID: 36033623	2022
Clinical-radiomics nomogram for identifying HER2 status in patients with breast cancer: A multicenter study. Frontiers in oncology\| PubMed ID: 36158700	2022
Using the metabolite alterations monitoring the AEG-1 expression level and cell biological behaviour of U251 cell in vitro. PloS one\| PubMed ID: 37656734	2023

Di Yin

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