artificial-intelligencedriven-personalized-learning-improves

Artificial Intelligence&#8211;Driven Personalized Learning Improves Operating Room Instrument Training: A Prospective Observational Study

Traditional operating room instrument training often relies on one-size-fits-all teaching, produces poor long-term skill retention, and contributes to ...

Zhejiang University School of Medicine

Peptide recognition through the MHC class I molecule by cytotoxic T lymphocytes (CTLs) leads to the killing of cancer cells. A potential challenge for T-cell immunotherapy is that dendritic cells (DCs) are exposed to the MHC class I-peptide complex for an insufficient amount of time. To improve tumour antigen presentation to T cells and thereby initiate a more effective T-cell response, we generated artificial antigen-presenting cells (aAPCs) by incubating human immature DCs (imDCs) with poly(lactic-co-glycolic) acid nanoparticles (PLGA-NPs) encapsulating tumour antigenic peptides, followed by maturation with lipopolysaccharide. Tumour antigen-specific CTLs were then induced using either peptide-loaded mature DCs (mDCs) or aAPCs, and their activities were analysed using both ELISpot and cytotoxicity assays. We found that the aAPCs induced significantly stronger tumour antigen-specific CTL responses than the controls, which included both mDCs and aAPCs loaded with empty nanoparticles. Moreover, frozen CTLs that were generated by exposure to aAPCs retained the capability to eradicate HLA-A2-positive tumour antigen-bearing cancer cells. These results indicated that aAPCs are superior to DCs when inducing the CTL response because the former are capable of continuously presenting tumour antigens to T cells in a sustained manner. The development of aAPCs with PLGA-NPs encapsulating tumour antigenic peptides is a promising approach for the generation of effective CTL responses in vitro and warrants further assessments in clinical trials.

Artificial human antigen-presenting cells are superior to dendritic cells at inducing cytotoxic T-cell responses.

Prone positioning during general anaesthesia is one of the most difficult practices for the perioperative nurse. Patients in this position are vulnerable to many preventable complications. However, no studies have developed an evidence-based tool to improve nursing practice during general anaesthesia and prone positioning. This study aimed to develop and test a general anaesthesia and prone position nursing checklist for use by the circulating nurse.

An evidence-based general anaesthesia and prone position nursing checklist: Development and testing.

Ovarian cancer (OC) ranks as the fifth most prevalent neoplasm in women and exhibits an unfavorable prognosis. To improve the OC patient's prognosis, a pioneering risk signature was formulated by amalgamating disulfidptosis-related genes.

Identification and validation of immunity- and disulfidptosis-related genes signature for predicting prognosis in ovarian cancer.

To compare the effectiveness of Chinese Association of Operating Room Nurses (CORN)-based nursing care against that of routine nursing for reducing intraoperative pressure injury (PI) in patients undergoing neurosurgery.

Comparison of Effectiveness of Chinese Association of Operating Room Nurses-Based Nursing Care and Routine Nursing in Reducing Intraoperative Pressure Injury in Patients Undergoing Neurosurgery.

Decreased ovarian reserve (DOR) refers to the decreased ability of the ovaries to produce eggs and the low quality of the follicles, which may lead to abnormal menstruation and infertility. However, the role of circular RNAs (circRNAs) in DOR remains poorly understood. Through circRNA sequencing, hsa_circ_0005379 was identified as the most significantly upregulated circRNA in the follicular fluid exosomes of patients with DOR. The present study aimed to characterize the circRNA expression profile and investigate the function of hsa_circ_0005379 in DOR. Exosomes were isolated from the follicular fluid of individuals with DOR and healthy controls, followed by sequencing using the Illumina HiSeq platform. Functional enrichment analysis, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis, was performed on the differentially expressed circRNAs. To further validate the results, the expression of five circRNAs was assessed in follicular fluid via reverse transcription-quantitative PCR (RT-qPCR). A DOR cell model was established by treating KGN granulosa cells with cyclophosphamide (CTX). Cell viability was evaluated using a Cell Counting Kit-8 assay following CTX exposure. hsa_circ_0005379 was either overexpressed or knocked down in the CTX-treated cells, and the levels of cell apoptosis, reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) were analyzed by flow cytometry and biochemical assays. Eight differentially expressed circRNAs were identified in individuals with DOR compared with controls, including seven upregulated and one downregulated. RT-qPCR confirmed that the expression trends of hsa_circ_0000344, hsa_circ_0001126, hsa-circ_0005379, hsa_circ-0005777 and hsa_circ_0007509 aligned with those from the sequencing results. Furthermore, overexpression and knockdown of hsa_circ_0005379 in KGN cells was verified by RT-qPCR. Silencing hsa_circ_0005379 in CTX-treated KGN cells promoted cell viability and reduced apoptosis, accompanied by reduced ROS and MDA levels and enhanced SOD activity. Overexpression of hsa_circ_0005379 yielded the opposite results. Collectively, the results suggested that hsa_circ_0005379 played a key role in regulating cell viability and oxidative stress in CTX-treated granulosa cells.

Circular RNA expression profiling and the potential role of hsa_circ_0005379 in decreased ovarian reserve.

Circular RNAs (circRNAs) are implicated in polycystic ovary syndrome (PCOS), yet their roles in granulosa cell dysfunction remain unclear. This study explores the mechanistic role of circ_0075691 in PCOS-associated metabolic dysregulation.

Circ_0075691 regulates lipid metabolism in granulosa cells by interacting with EIF4A3 to promote PTGS2 mRNA stability.

Multidisciplinary expert evaluation of large language models on questions regarding bariatric surgery: a comparative analysis of ERNIE Bot 4.0, ChatGPT-4, Claude 3 Opus, and Gemini Pro.

Article	Year
Artificial human antigen-presenting cells are superior to dendritic cells at inducing cytotoxic T-cell responses. Immunology\| PubMed ID: 28664991	2017
An evidence-based general anaesthesia and prone position nursing checklist: Development and testing. Nursing open\| PubMed ID: 36168198	2023
Identification and validation of immunity- and disulfidptosis-related genes signature for predicting prognosis in ovarian cancer. Heliyon\| PubMed ID: 38952356	2024
Comparison of Effectiveness of Chinese Association of Operating Room Nurses-Based Nursing Care and Routine Nursing in Reducing Intraoperative Pressure Injury in Patients Undergoing Neurosurg... Advances in skin & wound care\| PubMed ID: 40131875	2025
Circular RNA expression profiling and the potential role of hsa_circ_0005379 in decreased ovarian reserve. Experimental and therapeutic medicine\| PubMed ID: 41018883	2025
Circ_0075691 regulates lipid metabolism in granulosa cells by interacting with EIF4A3 to promote PTGS2 mRNA stability. Biochimica et biophysica acta. Molecular basis of disease\| PubMed ID: 41605366	2026
Multidisciplinary expert evaluation of large language models on questions regarding bariatric surgery: a comparative analysis of ERNIE Bot 4.0, ChatGPT-4, Claude 3 Opus, and Gemini Pro. Scientific reports\| PubMed ID: 41922459	2026

Jianshu Cai

Biography

JoVE Journal Publications

Other Publications