combined-laparoscopic-choledochoscopic-necrosectomy-for-infected

Combined Laparoscopic and Choledochoscopic Necrosectomy for Infected Pancreatic Necrosis: A Case Report

Infected pancreatic necrosis often necessitates intervention when initial step-up management fails. We present the case of a 63-year-old female with ...

Southern University of Science and Technology

Transcription factors (TFs) have been introduced to drive the highly efficient differentiation of human-induced pluripotent stem cells (hiPSCs) into lineage-specific oligodendrocytes (OLs). However, effective strategies currently rely mainly on genome-integrating viruses. Here we show that a synthetic modified messenger RNA (smRNA)-based reprogramming method that leads to the generation of transgene-free OLs has been developed. An smRNA encoding a modified form of OLIG2, in which the serine 147 phosphorylation site is replaced with alanine, OLIG2, is designed to reprogram hiPSCs into OLs. We demonstrate that repeated administration of the smRNA encoding OLIG2  lead to higher and more stable protein expression. Using the single-mutant OLIG2 smRNA morphogen, we establish a 6-day smRNA transfection protocol, and glial induction lead to rapid NG2 OL progenitor cell (OPC) generation (>70% purity) from hiPSC. The smRNA-induced NG2 OPCs can mature into functional OLs in vitro and promote remyelination in vivo. Taken together, we present a safe and efficient smRNA-driven strategy for hiPSC differentiation into OLs, which may be utilized for therapeutic OPC/OL transplantation in patients with neurodegenerative disease.

Rapid differentiation of hiPSCs into functional oligodendrocytes using an OLIG2 synthetic modified messenger RNA.

Publisher Correction: Rapid differentiation of hiPSCs into functional oligodendrocytes using an OLIG2 synthetic modified messenger RNA.

To identify a replacement strategy for bronchial thermoplasty (BT) with non-invasive and free-of-severe side effect is urgently needed in the clinic for severe asthma treatment. In this study, PLGA-PEG@ICG@TRPV1 pAb (PIT) photothermal nanoparticles targeting bronchial TRPV1 were designed for photothermal therapy (PTT) against severe murine asthma induced by ovalbumin and lipopolysaccharide. PIT was formulated with a polyethylene glycol (PEG)-grafted poly (lactic-co-glycolic) acid (PLGA) coating as a skeleton structure to encapsulate indocyanine green (ICG) and was conjugated to the polyclonal antibody against transient receptor potential vanilloid 1 (TRPV1 pAb). The results revealed that PIT held good druggability due to its electronegativity and small diameter. PIT demonstrated great photothermal effects both in vivo and in vitro and exhibited good ability to target TRPV1 in vitro because of its selective cell uptake and specific cell toxicity toward TRPV1-overexpressing cells. The PIT treatment effectively reduced asthma symptoms in mice. This is evident from improvements in expiratory airflow limitation, significant decreases in inflammatory cell infiltration in the airways, and increases in goblet cell and columnar epithelial cell proliferation. In conclusion, PIT alleviates severe murine asthma symptoms through a combination of TRPV1 targeting and photothermal effects.

Novel indocyanine green-loaded photothermal nanoparticles targeting TRPV1 for thermal ablation treatment of severe murine asthma induced by ovalbumin and lipopolysaccharide.

Fallopia aubertii (L. Henry) Holub (F. aubertii), a traditional Tibetan medicine, is used in China for treating respiratory inflammatory diseases, including acute lung injury (ALI). However, the chemical constituents of F. aubertii and its anti-inflammatory mechanisms in the lungs remain poorly understood.

Multiomics and experimental approaches reveal the anti-acute lung injury effects of Fallopia aubertii (L. Henry) Holub extract via IL-17/NF-κB pathway inhibition.

Ischemic stroke is a global public health problem with limited effective therapeutic options. Panax notoginseng saponins (PNS), the active extract derived from the well-known medicinal material called San-Qi in Chinese, has been widely used in the intervention of ischemic stroke.

Panax notoginseng saponins against ischemic stroke: From molecule to clinic.

Chronic cough hypersensitivity is common across respiratory diseases and often occurs without airway inflammation, yet effective treatments remain limited. Nitric oxide (NO), an important endogenous signaling molecule and environmental pollutant, has been implicated in respiratory pathophysiology, but its role in cough hypersensitivity remains unclear.

Long-term nitric oxide exposure induces cough hypersensitivity via non-inflammatory activation of the HIF1α-TRPV1 pathway.

Fufang Danshen Tablet (FDT) is a traditional Chinese medicine (TCM) formula with remarkable efficacy in invigorating blood and eliminating blood stasis, thus commonly used in treating ischemic stroke. However, no systemic summary has been conducted to evaluate its efficacy yet. This study aims to determine the efficacy of FDT therapy for promoting the rehabilitation of ischemic stroke and to explore the potential pharmacological mechanism through an  approach.

The efficacy and potential pharmacological mechanism of Fufang Danshen Tablet in promoting the rehabilitation of ischemic stroke: a meta-analysis and  study.

To dissect how malignant versus infectious pleural disease reshapes local and systemic immunity, we compared lung adenocarcinoma with tuberculosis using matched pleural effusions and peripheral blood from patients and healthy controls using single cell transcriptome analysis and TCR clonotype tracking. Th1/17 polarized pro-inflammation dominating TB effusions, while their precursors, the recently activated CD4 T cells, adopt an antitumor trajectory in LUAD, but their recruitment and clonal expansion were effectively suppressed. In both diseases, CD8⁺ T cells diverged into GZMH⁺ and GZMK⁺ subsets. The former served as the antitumor killer cells but suppressed by multiple anti-inflammatory signals especially from the extracellular matrix in LUAD. The later could serve as stem-like precursors. MAITs and class-switched B cells both exhibited higher levels of activation and expanded in TB. Myeloid cells were mostly recovered from peripheral bloods and showed heightened activation in TB, upregulating TNFRSF14, TNFRSF13B, and pro-inflammatory cytokines (CXCL2, CXCL3, IL1), while LUAD featured immunosuppressive signals including MDK-NCL, FN1-CD44, and THBS1-CD47. Cell-cell communication highlighted LUAD-enriched outgoing interaction strengths by myeloid cells including monocytes and DCs, through IL16 and VISFATIN signaling, which strongly corelated with survivals. In conclusion, both pleural effusions and peripheral blood from TB and LUAD exhibit significant distinct immune landscapes. These results delineate disease-specific molecular mechanisms underlying immune regulation in pleural malignancies and infections.

Article	Year
Rapid differentiation of hiPSCs into functional oligodendrocytes using an OLIG2 synthetic modified messenger RNA. Communications biology\| PubMed ID: 36241911	2022
Publisher Correction: Rapid differentiation of hiPSCs into functional oligodendrocytes using an OLIG2 synthetic modified messenger RNA. Communications biology\| PubMed ID: 36329174	2022
Novel indocyanine green-loaded photothermal nanoparticles targeting TRPV1 for thermal ablation treatment of severe murine asthma induced by ovalbumin and lipopolysaccharide. International journal of pharmaceutics\| PubMed ID: 38181990	2024
Multiomics and experimental approaches reveal the anti-acute lung injury effects of Fallopia aubertii (L. Henry) Holub extract via IL-17/NF-κB pathway inhibition. Journal of ethnopharmacology\| PubMed ID: 39571698	2025
Panax notoginseng saponins against ischemic stroke: From molecule to clinic. Journal of ethnopharmacology\| PubMed ID: 41173082	2026
Long-term nitric oxide exposure induces cough hypersensitivity via non-inflammatory activation of the HIF1α-TRPV1 pathway. Frontiers in pharmacology\| PubMed ID: 41635485	2026
The efficacy and potential pharmacological mechanism of Fufang Danshen Tablet in promoting the rehabilitation of ischemic stroke: a meta-analysis and study. Frontiers in neurology\| PubMed ID: 41767015	2026
Single-cell dissection of pleural and systemic immunity uncovers pathogen-specific immune reprogramming in tuberculosis versus lung adenocarcinoma. Inflammation research : official journal of the European Histamine Research Society ... [et al.]\| PubMed ID: 41865158	2026

Yichu Nie

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