allergic-bronchopulmonary-aspergillosis-aided-bronchoalveolar-lavage

Allergic Bronchopulmonary Aspergillosis Aided by Bronchoalveolar Lavage: A Case Report

This protocol establishes a clear primary goal: to develop a structured, actionable diagnostic workflow that enables accurate differentiation of allergic ...

Fudan University

Dendrobine (DDB), an alkaloid isolated from the Chinese herb Dendrobium, has antioxidant and anti-inflammatory effects; however, whether DDB reduces oleic acid (OA)-induced lipid accumulation remains unclear. OA-induced lipid accumulation model of HepG2 cells were treated with DDB. Cellular lipid deposition was assessed by Oil Red O (ORO) staining and triglyceride and total cholesterol detection. RNA-Sequencing (RNA-seq), biological function analysis, and transcription factor (TFs) prediction were combined to identify key TF in the DDB-treated OA model. Finally, the roles of FOS and METTL14 were examined using a DDB-induced lipid accumulation model. DDB inhibited OA-induced lipid accumulation. We identified 895 differentially expressed genes (DEGs) that were mainly enriched in various biological processes of lipid synthesis and transport. Four transcription factors (SOX9, MLXIPL, FOS, and JUN) associated with lipid metabolism and FOS levels in the OA-induced lipid accumulation model after DDB treatment had the greatest changes in expression change. Overexpression of FOS alleviates the inhibitory effect of DDB on OA-induced lipid accumulation. METTL14 is a target gene of FOS, and simultaneous interference with METTL14 in cells with high FOS expression restored the alleviating effect of DDB on lipid accumulation. DDB alleviated OA-induced lipid accumulation by inhibiting the FOS/METTL14 pathway.

Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway.

Correction: Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway.

Phosphorene has attracted extensive research interest due to its anisotropic properties with rich tunabilities such as strongly layer-dependent and field-tunable band gaps. Revealing how the electronic structure evolves down to monolayer is critical for understanding its anisotropic properties and field tunability. Here, we report the hitherto unexplored electronic structure of monolayer phosphorene, as well as field tunability in few-layer phosphorene. A strongly enhanced mass anisotropy is observed in monolayer phosphorene, which clearly distinguishes it from bilayer and thicker flakes. Moreover, by introducing an external electric field upon doping, modifications of the mass anisotropy and energy spacing between subbands in few-layer phosphorene are further revealed. Our work reveals the unique electronic structure of monolayer phosphorene, highlighting the critical roles of interlayer coupling as well as external electric field in tailoring the band parameters in phosphorene.

Article	Year
Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway. Journal of molecular histology\| PubMed ID: 39136847	2024
Correction: Dendrobine alleviates oleic acid-induced lipid accumulation by inhibiting FOS/METTL14 pathway. Journal of molecular histology\| PubMed ID: 39298064	2024
Strongly Enhanced Mass Anisotropy in Monolayer Phosphorene and Field Tunability. Physical review letters\| PubMed ID: 40794092	2025

Hongyun Zhang

Biography

JoVE Journal Publications

Other Publications