multiplexed-immunofluorescence-assay-for-spatial-assessment

Multiplexed Immunofluorescence Assay for Spatial Assessment of Senescence Markers <em>in vivo</em>

Multiplexed immunofluorescence (IF) staining represents a step-change in sophistication over traditional IF imaging allowing sequential detection of ...

Queen Mary University of London

Senescence classification is an acknowledged challenge within the field, as markers are cell-type and context dependent. Currently, multiple morphological and immunofluorescence markers are required. However, emerging scRNA-seq datasets have enabled an increased understanding of senescent cell heterogeneity.

SenPred: a single-cell RNA sequencing-based machine learning pipeline to classify deeply senescent dermal fibroblast cells for the detection of an in vivo senescent cell burden.

Federica Mossa

Biography

JoVE Journal Publications

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