mechanisms-liancao-xueniao-capsules-treating-hematuria-associated

Mechanisms of Liancao Xueniao Capsules in Treating Hematuria Associated with IgA Nephropathy: Network Pharmacology and Experimental Validation

IgA nephropathy (IgAN) is a prevalent primary glomerular disease characterized by hematuria, proteinuria, and progressive renal dysfunction. Hematuria, a ...

predicting-validating-regulation-podocyte-injury-treatment-diabetic

Predicting and Validating the Regulation of Podocyte Injury and Treatment of Diabetic Kidney Disease by Yinhuo Tang

Diabetic kidney disease (DKD) is a chronic kidney disease characterized by massive proteinuria and decreased glomerular filtration rate. The main ...

mechanism-kemeng-fang-s-inhibition-podocyte-apoptosis-rats-with

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Membranous nephropathy (MN) is a common pathological type of adult nephrotic syndrome. Up to 20% of patients with MN develop end-stage renal disease ...

the Affiliated Hospital to Changchun University of Chinese Medicine

Changchun University of Chinese Medicine

Microbial transformation of ginsenosides to increase its pharmaceutical effect is gaining increasing attention in recent years. In this study, Cellulosimicrobium sp. TH-20, which was isolated from soil samples on which ginseng grown, exhibited effective ginsenoside-transforming activity. After protopanaxadiol (PPD)-type ginsenoside (Rb1) and protopanaxatriol (PPT)-type ginsenosides (Re and Rg1) were fed to C. sp. TH20, a total of 12 metabolites, including 6 new intermediate metabolites, were identified. Stepwise deglycosylation and dehydrogenation on the feeding precursors have been observed. The final products were confirmed to be rare ginsenosides Rd, GypXVII, Rg2 and PPT after 96 h transformation with 38-96% yields. The four products showed improved anti-inflammatory activities by using lipopolysaccharide (LPS)-induced murine RAW 264.7 macrophages and the xylene-induced acute inflammatory model of mouse ear edema. The results indicated that they could dramatically attenuate the production of TNF-α more effectively than the precursors. Our study would provide an example of a unique and powerful microbial cell factory for efficiently converting both PPD-type and PPT-type ginsenosides to rare natural products, which extends the drug candidates as novel anti-inflammatory remedies.

Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng.

In this work, a novel polysaccharide (named DBFM3) was isolated from mycelia of Bjerkandera fumosa by DEAE-32 and Sepharose CL-6B column chromatography. High-performance gel permeation chromatography showed that DBFM3 was homogeneous, with an average molecular weight of 1.8×10Da. Structural characteristics of the purified fraction were investigated by high-performance liquid chromatography (HPLC), FT-IR, and NMR. HPLC analysis indicated that DBFM3 was composed of mannose, galacturonic acid, and galactose at molar ratios of 1:18.16:0.702. Spectral analysis suggested that DBFM3 had (1→6), (1→3,6), (1→3) linkages and pyranose conformation. Antioxidant assay in vitro showed that DBFM3 exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activity, and has protective effect against DNA damage and damage to SH-SY5Y cells induced by HO. Immunological tests indicated that DBFM3 significantly increased lymphocyte proliferation in vitro. Furthermore, DBFM3 increased the proliferation of lymphocytes in the presence of concanavalin A or lipopolysaccharide as mitogen.

ArticleTotal : 3	Year
Publication title	2026
Publication title Cited by 1	2025
Publication title Cited by 3	2024

Article	Year
Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng. Scientific reports\| PubMed ID: 28273939	2017
Purification, characterization, and bioactivities of a polysaccharide from mycelial fermentation of Bjerkandera fumosa. Carbohydrate polymers\| PubMed ID: 28433144	2017

Fan Li

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