single-cell-rna-sequencing-and-analysis-of-human-pancreatic-islets

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Pancreatic islets comprise of endocrine cells with distinctive hormone expression patterns. The endocrine cells show functional differences in response to ...

Regeneron Pharmaceuticals, Inc

This systematic review and meta-analysis aimed to evaluate the efficacy of transcutaneous electronic acupoint stimulation (TEAS) on bone marrow suppression in patients with lung cancer after chemotherapy.

Transcutaneous electronic acupoint stimulation improves bone marrow suppression in lung cancer patients following chemotherapy: A systematic review and meta-analysis of randomized controlled trials.

Asthma poses a major threat to human health. The aim of this study was to identify genetic markers of severe asthma and analyze the relationship between key genes and immune infiltration. Differentially expressed genes (DEGs) were first screened by downloading the training set GSE69683 and validation set GSE137268 from the GEO dataset. SVM-RFE analysis and the LASSO regression model were used to screen key genes, and CIBERSORT was used to assess immune infiltration in the samples. A total of 20 DEGs were identified in this study, mainly enriched for lymph node-like receptors, b-cell receptors, and neutrophil extracellular trap pathway. Comparative validation set GSE137268 identified thioredoxin (TXN) and coagulation factor V (F5) were identified as diagnostic markers of severe asthma. CIBERSORT analysis revealed that TXN and F5 are associated with multiple immune cell infiltrates. In addition, we identified miRNA and TF at the transcriptional level that may regulate F5 and TXN, and found that several commonly used drugs may exert therapeutic effects by targeting F5 and TXN. Taken together, TXN and F5 may be key genes in the development of severe asthma and are associated with immune infiltration. Our study can help to better understand the pathogenesis of asthma and provide new ideas for clinical treatment.

Identification of TXN and F5 as novel diagnostic gene biomarkers of the severe asthma based on bioinformatics and machine learning analysis.

The bronchopleural fistula (BPF) is a pathological passageway between the bronchus and the pleural cavity. Diagnosing and localising BPF can be challenging, and the traditional retrograde methylene blue (MB) perfusion method may fail to identify multifocal BPFs. This article reports a novel method for locating multifocal BPFs in patients undergoing concurrent empyema debridement. Initially, selective endobronchial injection of MB solution is performed under bronchoscopic guidance. Subsequently, the thoracic surgeon determines the location of BPFs as the MB solution flows into the pleural cavity. This process of injecting MB within the bronchus is repeated to locate any additional BPFs until no new ones are found. We report a case where this new method was used to identify multifocal BPFs, ultimately diagnosing three BPFs' presence. Following treatment with bronchopleural fistula occlusion, no recurrence of BPFs was observed during the follow-up period.

Bronchoscopic-Guided Selective Endobronchial Injection of Methylene Blue Solution for Localising Peripheral Bronchopleural Fistulas During Pleural Debridement in a Post-Lobectomy Patient With Bronchopleural Fistula and Empyema.

Pneumonia Compound Formulation (PCF) is a traditional Chinese medicine (TCM) formula used for the clinical treatment of novel coronavirus pneumonia. However, its efficacy and mechanism of action for community-acquired pneumonia (CAP) are unknown. Therefore, the aim of this study was to evaluate the efficacy of PCF combined with antibiotics in the treatment of CAP and to explore its mechanism based on metabolomics.

Efficacy and metabolomic analysis of the pneumonia compound formulation against community-acquired pneumonia: an observational controlled before-after clinical trial.

Article	Year
Transcutaneous electronic acupoint stimulation improves bone marrow suppression in lung cancer patients following chemotherapy: A systematic review and meta-analysis of randomized controlled... Medicine\| PubMed ID: 37083807	2023
Identification of TXN and F5 as novel diagnostic gene biomarkers of the severe asthma based on bioinformatics and machine learning analysis. Autoimmunity\| PubMed ID: 39531229	2024
Bronchoscopic-Guided Selective Endobronchial Injection of Methylene Blue Solution for Localising Peripheral Bronchopleural Fistulas During Pleural Debridement in a Post-Lobectomy Patient Wit... Respirology case reports\| PubMed ID: 39850744	2025
Efficacy and metabolomic analysis of the pneumonia compound formulation against community-acquired pneumonia: an observational controlled before-after clinical trial. BMC infectious diseases\| PubMed ID: 40165069	2025

Yi Wei

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