capillary-electrophoresis-based-hydrogendeuterium-exchange-for

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Resolving conformational heterogeneity of multiple protein states that coexist in solution remains one of the main obstacles in the characterization of ...

nanjing normal university

Shenzhen Bay laboratory

As the largest group of mammalian species, which are also widely distributed all over the world, rodents are the natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding of the core virome of diverse rodents should therefore assist in efforts to reduce the risk of future emergence or re-emergence of rodent-borne zoonotic pathogens.

Decoding the RNA viromes in rodent lungs provides new insight into the origin and evolutionary patterns of rodent-borne pathogens in Mainland Southeast Asia.

DNA double-strand breaks (DSBs) are among the most deleterious DNA lesions, and they can cause cancer if improperly repaired. Recent chromosome conformation capture techniques, such as Hi-C, have enabled the identification of relationships between the 3D chromatin structure and DSBs, but little is known about how to explain these relationships, especially from global contact maps, or their contributions to DSB formation.

A graph neural network-based interpretable framework reveals a novel DNA fragility-associated chromatin structural unit.

The dismal prognosis of hepatocellular carcinoma (HCC) is closely associated with characteristics of the tumour microenvironment (TME). Recent studies have confirmed the presence and potential influence of the microbiome in TME on cancer progression. Elucidating the relationship between microbes in the TME and cancer could provide valuable insights into novel diagnostic markers and therapeutic strategies for HCC and thus warrants a closer investigation of the role of intratumoural microbiome in the HCC TME.

Intratumoural microbiome can predict the prognosis of hepatocellular carcinoma after surgery.

Robot-assisted laparoscopic partial nephrectomy (RAPN) has become a key technology in the treatment of renal tumors. Effective preoperative planning and precise intraoperative navigation are critical to a successful surgical outcome. This study aimed to evaluate the clinical application value of mixed reality (MR) in robotic nephron-sparing partial nephrectomy for patients with renal tumors of different complexity based on the R.E.N.A.L. score.

The clinical application value of mixed reality in robotic laparoscopic partial nephrectomy.

Papillary thyroid carcinoma (PTC) is the most common malignant thyroid cancer. Several clinicopathological features may affect therapeutic efficacy.

Correlation of TgAb With Clinicopathological Features and Unfavorable Efficacy of 131I Ablation in PTC.

Suboptimal fruit and vegetable intake remains a significant global public health concern. This study aimed to estimate the global intake and the disease burden attributable to suboptimal fruit and vegetable intake.

The global burden of disease attributable to suboptimal fruit and vegetable intake, 1990-2021: a systematic analysis of the global burden of disease study.

To define the prevalence and anatomical patterns of paranasal sinus abnormalities (PSA) in thyroid-associated ophthalmopathy (TAO) and to test the hypothesis that TAO is partially driven by contiguous orbital inflammation rather than systemic autoimmunity or generalized orbital pressure.

Contiguous orbital inflammation from paranasal sinus abnormalities in etiology of thyroid-associated ophthalmopathy.

The potential for microbial modulation to serve as both a therapeutic and prophylactic strategy against type 2 diabetes mellitus (T2DM)-induced microvasculopathy remains underexplored. We therefore compared the efficacy of the gut-centric herbal formulation Intestinal Flora Balance (IFB, BNFF™), when used for therapeutic reversal, synergistic rescue, or prophylactic protection.

Modulation of gut microbiota by the herbal formulation Intestinal Flora Balance rescues pancreatic and intestinal microvascular dysfunction to alleviate type 2 diabetes mellitus.

Renal microvascular dysfunction is a central driver of diabetic kidney disease, yet the potential for rapid therapeutic reversal remains obscure. We investigated the effect of short-term liraglutide on early changes in the renal microcirculation post-induction of diabetes.

Rapid improvement of renal microcirculatory homeostasis by liraglutide following diabetes induction.

The microcirculation is a determinant of organ function, translating systemic signals into local physiological responses. However, whether the regulation of microhemodynamics is sexually dimorphic within the pancreas has remained unknown. Here, using a multi-scale approach in healthy mice, we report a sexual dimorphism in pancreatic microhemodynamics that is anatomically restricted to the exocrine compartment, defined by divergent expression of the endothelial marker CD31 and estrogen receptor ERβ, whereas the endocrine islet microvasculature remains conserved between sexes. We demonstrate that microhemodynamic dimorphism is functionally coupled to divergent systemic steroid hormone profiles, a male signature characterized by elevated androgens and steroidogenic precursors, and a female signature dominated by glucocorticoids and estrogen metabolites. The distinction manifests as fundamentally different regulatory principles for vascular control, where pancreatic blood flow in males is directly coupled to systemic blood pressure, whereas in females, the relationship is defined by correlations between blood pressure and the oscillatory components of microhemodynamics. Collectively, our data identify the pancreatic microcirculation as a primary site of physiological sexual dimorphism and establish its role as a fundamental conduit for translating the systemic hormonal milieu into sex-specific organ homeostasis.

Functional Segregation of Pancreatic Microcirculation Reveals Sex-Dependent Microhemodynamic Signatures.

The integrity of the pancreatic islet microvasculature is critical for endocrine function, yet it is progressively compromised by glucotoxicity in diabetes. While matrix metalloproteinases are implicated, the role of stromelysin-1 as a potential upstream driver of endothelial dysfunction remains poorly defined. The aim of our study was to elucidate the role of stromelysin-1 in mediating glucotoxic injury to islet microvascular endothelial cells (IMECs). To this end, we employed a CRISPR/Cas9-mediated knockout of stromelysin-1 in IMECs. Cellular functions, including proliferation, migration, and angiogenesis, were assessed using IncuCyte ZOOM live-cell imaging, while endothelial barrier integrity was quantified via a 40 kDa dextran flux assay. Additionally, the secretome was profiled using a cytokine antibody array. We found that genetic ablation of stromelysin-1 conferred protection against glucotoxicity. Stromelysin-1 KO IMECs exhibited significantly enhanced proliferation, migration, and angiogenic capacity compared to wild-type controls. Furthermore, stromelysin-1 deficiency restored endothelial monolayer integrity by attenuating high-glucose-induced hyperpermeability. These functional improvements were linked to a remodelling of the secretome, characterised by decreased secretion of the pro-degradative MMP-2 and increased secretion of the anti-inflammatory cytokine IL-10 and the endogenous inhibitor TIMP-2. Overall, our findings establish stromelysin-1 as a crucial mediator of glucotoxic injury in islet microvascular endothelial cells.

Consequences of CRISPR-Cas9-Mediated Stromelysin-1 Knockout in Pancreatic Islet Microvascular Endothelial Cells.

Optimal management of T1-2 non-ampullary duodenal neuroendocrine tumors (NAD-NETs) remains controversial, particularly for intermediate-sized tumors (11-20 mm), due to limited evidence. This study aimed to quantify real-world metastasis risk and compare long-term survival after endoscopic therapy (ET) or surgical therapy (ST) for T1-2N0M0 NAD-NETs.

Risk of metastasis and survival in patients with T1-2 non-ampullary duodenal neuroendocrine tumors undergoing different treatment strategies.

Chronic enteropathies characterized by multiple superficial ulcers of the small intestine have long been under-recognized, particularly in their early stage. However, the occurrence of refractory occult bleeding and episodes of partial bowel obstruction in this disease severely impacts quality of life, while targeted therapeutic options remain limited. Although dysfunction of the prostaglandin metabolic pathway has been associated with mucosal damage, the underlying molecular mechanisms and potential therapeutic targets remain unclear. In recent years, in-depth investigations of nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy, along with the discovery of rare monogenic disorders affecting the prostaglandin metabolic pathway, have helped bridge this knowledge gap. A broader concept, termed "prostaglandin-associated enteropathy (PGAE)", has since emerged, representing a monumental breakthrough in the differential diagnosis of nonspecific small intestinal ulcers. This narrative review focuses on prostaglandin metabolism and chronic intestinal ulcers, including NSAID-induced enteropathy and chronic enteropathies associated with solute carrier organic anion transporter family member 2A1 () and phospholipase A2 group IVA (). By unraveling molecular connections and highlighting innovative therapeutic avenues, we aim to advance clinical management and improve the well-being and quality of life for patients with PGAE.

Article	Year
Decoding the RNA viromes in rodent lungs provides new insight into the origin and evolutionary patterns of rodent-borne pathogens in Mainland Southeast Asia. Microbiome\| PubMed ID: 33478588	2021
A graph neural network-based interpretable framework reveals a novel DNA fragility-associated chromatin structural unit. Genome biology\| PubMed ID: 37095580	2023
Intratumoural microbiome can predict the prognosis of hepatocellular carcinoma after surgery. Clinical and translational medicine\| PubMed ID: 37462602	2023
The clinical application value of mixed reality in robotic laparoscopic partial nephrectomy. Frontiers in oncology\| PubMed ID: 39568562	2024
Correlation of TgAb With Clinicopathological Features and Unfavorable Efficacy of 131I Ablation in PTC. The Journal of clinical endocrinology and metabolism\| PubMed ID: 39918837	2025
The global burden of disease attributable to suboptimal fruit and vegetable intake, 1990-2021: a systematic analysis of the global burden of disease study. BMC medicine\| PubMed ID: 40764991	2025
Contiguous orbital inflammation from paranasal sinus abnormalities in etiology of thyroid-associated ophthalmopathy. International journal of ophthalmology\| PubMed ID: 41524016	2026
Modulation of gut microbiota by the herbal formulation Intestinal Flora Balance rescues pancreatic and intestinal microvascular dysfunction to alleviate type 2 diabetes mellitus. Phytomedicine : international journal of phytotherapy and phytopharmacology\| PubMed ID: 41544466	2026
Rapid improvement of renal microcirculatory homeostasis by liraglutide following diabetes induction. Diabetes, obesity & metabolism\| PubMed ID: 41749403	2026
Functional Segregation of Pancreatic Microcirculation Reveals Sex-Dependent Microhemodynamic Signatures. Comprehensive Physiology\| PubMed ID: 41846427	2026
Consequences of CRISPR-Cas9-Mediated Stromelysin-1 Knockout in Pancreatic Islet Microvascular Endothelial Cells. Journal of cellular and molecular medicine\| PubMed ID: 41860163	2026
Risk of metastasis and survival in patients with T1-2 non-ampullary duodenal neuroendocrine tumors undergoing different treatment strategies. Discover oncology\| PubMed ID: 41986810	2026
What we know about prostaglandin pathway dysfunction in chronic enteropathies: From endoscopy to molecular diagnosis. Journal of translational internal medicine\| PubMed ID: 42046811	2026

Xiang Xu

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