molecular-profiling-mucinous-pancreatic-cystic-neoplasms-implications

Molecular Profiling in Mucinous Pancreatic Cystic Neoplasms: Implications for Surgical Decision-Making &#8212; A Narrative Review

Pancreatic cystic lesions (PCLs) represent a heterogeneous group ranging from benign cysts to lesions with malignant potential. Despite advances in ...

The First Hospital of Jilin University

Nanopipettes provide a promising confined space that enables advances in electrochemical, optical, and mass spectrometric measurements at the nanoscale. They have been employed to reveal the hidden population properties and dynamics of single molecules and single particles. Moreover, new detection mechanisms based on nanopipettes have led to detailed information on single cells at high spatial and temporal resolution. In this Minireview, we focus on the fabrication and characterization of nanopipettes, summarize their wide applications for the analysis of single entities, and conclude with an outlook for advanced practical sensing.

Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells.

Aggregation-induced emission (AIE) as a unique photophysical process has been intensively explored for their features in fields from optical sensing, bioimaging to optoelectronic devices. However, all AIE luminogens (AIEgens) hardly recover into the initial dispersed state after illuminating at the ultimate aggregated state, which limits AIEgens to achieve reversible sensing and reproducible devices. To real-time manipulate the emission of AIEgen, here we take the advantage of confined space in the quartz nanopore to achieve a nanopore-size-dependent restriction of AIEgens for reversible conversions of "on-to-off" and "off-to-on" emission. By electrochemically manipulating 26 fL AIEgen solution inside nanopore confinement, AIE illuminates while moves along nanopore from the constricted tip to inside cavity at a velocity of 1.4-2.2 μm s, and vice versa. We further apply this dynamic manipulation for a target delivery of AIEgen into single cells, which opens up new possibility to design powerful and practical AIE applications.

Manipulating and visualizing the dynamic aggregation-induced emission within a confined quartz nanopore.

The evolution of the nanobubble from gas molecules in liquid phase is of fundamental interest. However, the lack of sensitive tools hinders the study of growth dynamic of the bubble at nanoscale. Here, we employed a confined glass nanopore to real-time monitor the dynamics behavior of a single nanobubble generated by the reaction between NaBH and HO. By analyzing the characteristic ionic current signal, the formation time and growth time of a single nanobubble could be estimated as 200 and 21 ms, respectively. Further, the nanopore size has been altered to modulate the growth behavior of the nanobubble. The results demonstrate the capability of the nanopore for sensitively tracking the behavior of single nanobubbles in liquid phase, which provides a powerful method for further understanding nanobubble evolution.

Article	Year
Confined Nanopipette Sensing: From Single Molecules, Single Nanoparticles, to Single Cells. Angewandte Chemie (International ed. in English)\| PubMed ID: 30066493	2019
Manipulating and visualizing the dynamic aggregation-induced emission within a confined quartz nanopore. Nature communications\| PubMed ID: 30194303	2018
Characterization of the Dynamic Growth of the Nanobubble within the Confined Glass Nanopore. Analytical chemistry\| PubMed ID: 30295035	2018

Rui Gao

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