a-magnetic-bead-based-negative-selection-flow-cytometry-protocol-for

A Magnetic Bead-Based Negative Selection and Flow Cytometry Protocol for Evaluating Licochalcone A-Mediated Inhibition of Murine Th17 Differentiation

Na&#239;ve CD4&#8314; T cell differentiation into Th17 cells is a key driver of autoimmune and chronic inflammatory skin diseases. Licochalcone A ...

The First Affiliated Hospital of Chongqing College of Traditional Chinese Medicine

Microcephalin (MCPH1/BRIT1) is a large nuclear protein that is involved in the early cellular response to DNA damage, the expression of which is reduced in a variety of types of human tumors. A recent study by our group demonstrated that MCPH1 expression is markedly decreased in lung cancer. However, it remains unclear whether inducing the expression of MCPH1 may ameliorate lung cancer, and, if so, which mechanisms underlie this process. The results of the present study demonstrated that MCPH1 expression was downregulated in lung cancer tissues compared with that in normal lung tissues. Furthermore, MCPH1 overexpression in A549 non-small cell lung carcinoma cells, successfully inhibited cell proliferation via arrest of the cell cycle in the S and G2/M phases. In addition, MCPH1 overexpression promoted cell apoptosis, in association with a significant increase in the quantities of Bax and active caspase-3, as well as a decrease in the level of Bcl-2. In conclusion the present results indicated that MCPH1 is involved in the regulation of apoptosis and entry into mitosis, suggesting that MCPH1 may function as a tumor suppressor and that it may be important in the pathogenesis of lung cancer.

Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells.

We report the clinical features and genetic testing of a child with Smith-Magenis syndrome (SMS) to improve the understanding of this disease. The clinical data and molecular genetic test results of a child with SMS caused by a novel mutation in the retinoic acid-induced-1 (RAI1) gene were reviewed. A female patient aged 12 years and 9 months presented to the clinic because her mental and motor development was lagging behind that of her peers. The child had learning difficulties, poor motor coordination, temper tantrums, and self-injurious behaviors, such as skin scratching. She had a peculiar facial appearance, dry skin with scattered eczema, low hairline, wide forehead, flat face, collapsed nasal bridge, turned out upper lip, and deep palmar lines on the right hand through the palm. Wechsler's IQ test score was 48. Her electroencephalogram was normal. The diagnosis of SMS was confirmed by a heterozygous mutation in exon 3 of the RAI1 gene on chromosome chr-1717696650 at locus c.388C>T (P.Q130X). In addition, this patient had severe eczema on the skin. The RAI1 mutation c.388C>T (P.Q130X) is a newly reported variant that will help in the clinical identification of SMS and the precise localization of more phenotypically related genes.

Article	Year
Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells. Oncology letters\| PubMed ID: 26870219	2016
A case of Smith-Magenis syndrome with skin manifestations caused by a novel locus mutation in the RAI1 gene. The Journal of international medical research\| PubMed ID: 37756600	2023
Kaposi's varicelliform eruption in a patient with acne vulgaris: A case report. Journal of cosmetic dermatology\| PubMed ID: 37955160	2023
Treatment of erythromelanosis follicularis faciei et colli with a 595-nm pulsed dye laser. Journal of cosmetic dermatology\| PubMed ID: 37969063	2023

Yanxi Li

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