salvianolic-acid-b-suppresses-epithelial-mesenchymal-transition

Salvianolic Acid B Suppresses Epithelial-Mesenchymal Transition-Associated Phenotypes in Cervical Cancer Cells via GPIb Alpha/LYN/TWIST1 Signaling

Cervical cancer remains a major gynecological malignancy, and the epithelial-mesenchymal transition is associated with tumor progression. Salvianolic acid ...

Anhui medical university

Small cell neuroendocrine carcinoma of the cervix (SCNEC) is a rare and highly aggressive malignancy with a poor prognosis. We report the case of a 36-year-old nulliparous woman with a history of HPV-18 infection and repeated cytology showing atypical squamous cells of undetermined significance (ASC-US), while multiple biopsies remained negative. She initially underwent surgery for multiple uterine leiomyomas and severe endometriosis. Ten months later, she presented with acute pelvic pain, rectal pressure, and minor vaginal bleeding. Combined hysteroscopy and laparoscopy revealed suspicious lesions, and histopathology with immunohistochemistry confirmed International Federation of Gynecology and Obstetrics (FIGO) stage IVB SCNEC. Postoperatively, she received platinum-based chemotherapy followed by salvage immunotherapy. Despite a transient decline in tumor markers, the disease progressed rapidly, with enlargement and confluence of pelvic lesions and extensive bone metastases, ultimately resulting in death from multiorgan failure. This case highlights the diagnostic challenges, early systemic dissemination, and poor outcomes of SCNEC, underscoring the urgent need for more effective therapeutic strategies.

Case Report: Diagnostic challenges in cervical small cell neuroendocrine carcinoma of a reproductive-age woman.

Cervical cancer remains a major cause of cancer-related morbidity and mortality in women, particularly among patients diagnosed with locally advanced, recurrent, or metastatic disease. Here, we investigated the role of transcriptional repressor GATA-binding 1 (TRPS1) in cervical cancer progression and defined its downstream regulatory mechanism. Bioinformatics analysis revealed differential expression of TRPS1 between cervical cancer and normal cervical tissues. To further explore its functional role, TRPS1 was overexpressed in cervical cancer cell lines via lentiviral infection. In vitro and in vivo experiments confirmed that TRPS1 expression was downregulated in cervical cancer, and its overexpression inhibited cervical cancer cell proliferation, migration, invasion, and stemness, as well as tumor growth in xenograft models. Mechanistically, ORC6 was identified as a candidate downstream effector of TRPS1. TRPS1 bound to the ORC6 promoter and repressed ORC6 transcription, leading to reduced ORC6 expression. Functional rescue experiments showed that ORC6 restoration partially reversed TRPS1-mediated inhibition of cell-cycle progression, apoptosis resistance, stemness-marker expression, EMT-associated marker expression, and tumor growth. Notably, low TRPS1 expression and high ORC6 expression were associated with lymphovascular invasion and lymph node metastasis in cervical cancer patients. Together, these findings identify a TRPS1-ORC6 regulatory axis that restrains cervical cancer stemness and progression.

Article	Year
Case Report: Diagnostic challenges in cervical small cell neuroendocrine carcinoma of a reproductive-age woman. Frontiers in oncology\| PubMed ID: 41293259	2025
TRPS1 inhibits cervical cancer progression by suppressing ORC6-mediated tumor stemness. Tissue & cell\| PubMed ID: 42247779	2026

Yichen Feng

Biography

JoVE Journal Publications

Other Publications