a-standardized-vitro-protocol-for-evaluating-human-dental-pulp-stem

A Standardized <em>In Vitro</em> Protocol for Evaluating Human Dental Pulp Stem Cell Differentiation and Proliferation on Nanomaterial Scaffolds

Reliable assessment of osteogenic and odontogenic differentiation, as well as stem cell proliferation, is fundamental to evaluating the biological ...

Ain Shams University

This study evaluated the sealing ability of ProRoot MTA and Biodentine as root-end filling materials.

Sealing ability of Biodentine versus ProRoot mineral trioxide aggregate as root-end filling materials.

Propolis is a unique natural adhesive product collected by honeybees. It contains a diversity of bioactive compounds with reported functional properties such as antioxidants, antibacterial, antifungal, anti-inflammatory, antiviral and anticancer activity. Dental caries is a worldwide problem that caused by microbial growth usually progress from tooth enamel to the underlying pulpal tissues and root canal. This situation could be controlled by a sequence of steps to remove microorganisms and fill root canal with a suitable long-lasting root canal sealer. Unfortunately, leachable and degradation products of the currently used sealers compromised their antimicrobial activity by inflammatory modulation associated with irritation and toxicity of periapical tissues.

Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer.

This study evaluated the biocompatibility of mineral trioxide aggregate (MTA) and Biodentine (BD) as root-end filling materials. Six mongrel dogs were divided into two equal groups according to the evaluation period; group A: one month and group B: three months. Three premolars of the same quadrant in each arch were used, summing up 36 teeth (6 teeth/dog). These teeth were randomly subdivided into three subgroups according to the root-end filling material used: MTA, BD and no root-end filling material (control). Endodontic access cavities were performed for induction of periapical pathosis. After the infection period, root canal instrumentation and obturation were accomplished. One day after root canal procedures, root-end surgery was performed. Surgical access was achieved and the root-end was resected approximately 3 mm above the apex. Root-end cavity was prepared ultrasonically and filled with the tested materials. All samples were evaluated by radiography and histopathology (Inflammation and new hard tissue formation). Data were collected and subjected to statistical analysis. In group A, MTA subgroup exhibited significant higher mean inflammatory score than BD subgroup (P < 0.05) while no significant difference was recorded between MTA and BD subgroups in group B (P > 0.05). Regarding mean mineralization score, there was no significant difference between all subgroups in both groups A and B (P > 0.05). Biodentine exhibited favorable biocompatibility in the initial stage of healing than MTA and comparable biomineralization. Clinical relevance: Biodentine could be considered as an acceptable alternative to MTA in peri-radicular surgeries.

Biocompatibility of mineral trioxide aggregate and biodentine as root-end filling materials: an in vivo study.

 The aim of this study was to evaluate the osteogenic differentiation ability and proliferation of apical papilla stem cells (SCAPs) using chitosan-coated nanohydroxyapatite and bioactive glass nanoparticles.

Osteogenic Differentiation and Proliferation of Apical Papilla Stem Cells Using Chitosan-Coated Nanohydroxyapatite and Bioactive Glass Nanoparticles.

Regenerative endodontics' primary objective is to establish a favorable environment in the root canal by removing infection, providing a sturdy scaffold, and sealing the apical end of the tooth tightly. These actions should promote pulp regeneration and root development.

Histological evaluation of the regenerative potential of injectable hyaluronic acid hydrogel or collagen with blood clot as scaffolds during revascularization of immature necrotic dog's teeth.

This study aimed to assess the biocompatibility and osteoconductivity of a resin-based and a resin-free calcium silicate sealer and their ability to promote healing of induced periapical lesions in mature dogs' teeth.

Evaluation of osteoconductivity and inflammatory response of a resin-based and a resin-free calcium silicate sealer: an in vivo study.

Bacterial infection in root canals often persist despite thorough chemomechanical preparation. Conventional irrigants and medicaments reduce biofilms but have been accused of weakening dentin. Amorphous calcium phosphate nanoparticles showed promising results in enhancing dentin hardness, but the formula's anticmicrobial effect is yet to be evaluated in endodontic contexts. The aim of this study was to evaluate the feasibility of using 20% amorphous calcium phosphate nanoparticles loaded in chlorhexidine as an antibacterial intracanal medicament in endodontics.

Preliminary in-vitro antibacterial evaluation of a novel formula of calcium phosphate nanoparticle-chlorhexidine intracanal medicament using confocal laser scanning microscopy.

A hermetic seal is essential for successful root canal therapy. Sealers fill the interface between gutta-percha and dentin, improving adaptation and sealing ability. This study aimed to evaluate the effect of heat on the push-out bond strength and failure modes of calcium silicate- and epoxy resin-based sealers.

Article	Year
Sealing ability of Biodentine versus ProRoot mineral trioxide aggregate as root-end filling materials. The Saudi dental journal\| PubMed ID: 30723363	2019
Design, Evaluation And Antimicrobial Activity Of Egyptian Propolis-Loaded Nanoparticles: Intrinsic Role As A Novel And Naturally Based Root Canal Nanosealer. International journal of nanomedicine\| PubMed ID: 31695372	2019
Biocompatibility of mineral trioxide aggregate and biodentine as root-end filling materials: an in vivo study. Scientific reports\| PubMed ID: 38347030	2024
Osteogenic Differentiation and Proliferation of Apical Papilla Stem Cells Using Chitosan-Coated Nanohydroxyapatite and Bioactive Glass Nanoparticles. European journal of dentistry\| PubMed ID: 38442913	2024
Histological evaluation of the regenerative potential of injectable hyaluronic acid hydrogel or collagen with blood clot as scaffolds during revascularization of immature necrotic dog's teet... Open veterinary journal\| PubMed ID: 39737041	2024
Evaluation of osteoconductivity and inflammatory response of a resin-based and a resin-free calcium silicate sealer: an in vivo study. Clinical oral investigations\| PubMed ID: 40467926	2025
Preliminary in-vitro antibacterial evaluation of a novel formula of calcium phosphate nanoparticle-chlorhexidine intracanal medicament using confocal laser scanning microscopy. BMC oral health\| PubMed ID: 42098696	2026
Comparative analysis of heat impacts on bond strength and failure modes of calcium silicate- and epoxy resin-based sealers. BMC oral health\| PubMed ID: 42144610	2026

Abeer A. Elgendy

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