bibliometric-analysis-traditional-chinese-medicine-derived-bioactive

Bibliometric Analysis of Traditional Chinese Medicine-Derived Bioactive Constituents in Macrophage Polarization Regulation

Macrophage polarization contributes to inflammatory regulation, tumor immunity, tissue repair, and fibrosis. However, the knowledge structure, research ...

State Key Laboratory for Innovation and Transformation of Luobing Theory

Bazi Bushen (BZBS), a traditional Chinese medicine, has been proven effective in the treatment of age-related disease in mouse models. However, whether its therapeutic effects are due to antiaging mechanism has not yet been explored. In the present study, we investigated the antiaging effects of BZBS in naturally aging mice by using behavioral tests, liver DNA methylome sequencing, methylation age estimation, and frailty index assessment. The methylome analysis revealed a decrease of mCpG levels in the aged mouse liver. BZBS treatment tended to restore age-associated methylation decline and prune the methylation pattern toward that of young mice. More importantly, BZBS significantly rejuvenated methylation age of the aged mice, which was computed by an upgraded DNA methylation clock. These results were consistent with enhanced memory and muscular endurance, as well as decreased frailty score and liver pathological changes. KEGG analysis together with aging-related database screening identified methylation-targeted pathways upon BZBS treatment, including oxidative stress, DNA repair, MAPK signaling, and inflammation. Upregulation of key effectors and their downstream effects on elevating Sod2 expression and diminishing DNA damage were further investigated. Finally, in vitro experiments with senescent HUVECs proved a direct effect of BZBS extracts on the regulation of methylation enzymes during cellular aging. In summary, our work has revealed for the first time the antiaging effects of BZBS by slowing the methylation aging. These results suggest that BZBS might have great potential to extend healthspan and also explored the mechanism of BZBS action in the treatment of age-related diseases.

Bazi Bushen mitigates epigenetic aging and extends healthspan in naturally aging mice.

Chronic inflammation and metabolic dysfunction are key features of systemic aging, closely associated with the development and progression of age-related metabolic diseases. Bazi Bushen (BZBS), a traditional Chinese medicine used to alleviate frailty, delays biological aging by modulating DNA methylation levels. However, the precise mechanism of its anti-aging effect remains unclear. In this study, we developed the Energy Expenditure Aging Index (EEAI) to estimate biological age. By integrating the EEAI with transcriptome analysis, we aimed to explore the impact of BZBS on age-related metabolic dysregulation and inflammation in naturally aging mice.

Bazi Bushen ameliorates age-related energy metabolism dysregulation by targeting the IL-17/TNF inflammatory pathway associated with SASP.

Acute primary angle closure (APAC) is an emergency ophthalmic presentation and a major cause of irreversible blindness in China. However, only a few studies have focused on the characteristics of optic disc hemorrhage (ODH) during an APAC attack, including its shape, depth, location, scope, and duration after intraocular pressure (IOP) control, along with changes in the optic nerve. This study aimed to analyze the characteristics of ODH and optic nerve changes in patients during their first APAC episode.

Characteristics of optic disc hemorrhage and optic nerve changes following acute primary angle closure.

Alzheimer's disease (AD) is the most common and severe degenerative disorder of the central nervous system in the elderly, profoundly impacting patients' quality of life. However, effective therapeutic agents for AD are still lacking. Bazi Bushen capsule (BZBS) is a traditional Chinese herbal compound with potential neuroprotective effects, yet its underlying mechanisms remain poorly understood.

Targeting LKB1-AMPK-SIRT1-induced autophagy and mitophagy pathways improves cerebrovascular homeostasis in APP/PS1 mice.

To explore the intrinsic relationship between benign prostatic hyperplasia (BPH) and erectile dysfunction (ED) and evaluate the therapeutic effects of Bazi Bushen Capsule (BZBS).

Intervention of Bazi Bushen Capsule on Benign Prostatic Hyperplasia Concomitant with Erectile Dysfunction Induced by Testosterone Supplementation in Spontaneously Hypertensive Rats.

To evaluate the 2-year outcomes of goniotomy (GT) in patients with prior failed glaucoma surgery.

Two-Year Outcomes of Goniotomy After Failed Surgery for Glaucoma: A Multicenter Study.

Photoaging is a prevalent and multifaceted form of exogenous skin aging. A comprehensive approach to preventing photoaging may yield greater benefits compared to a single intervention.

Bazi Bushen capsule alleviates skin photoaging by targeting the KEAP1-NRF2 pathway.

This study aims to investigate how hyperoside (HYP) alleviates oxidative stress-induced osteoporosis, its molecular mechanisms, and its impact on osteoblast differentiation, oxidative damage, and the estrogen-PI3K/VEGF signaling pathway.

Hyperoside Modulates the Estrogen-PI3K/VEGF Axis to Ameliorate Oxidative Damage-Induced Inhibition of Bone Formation in MC3T3-E1 Cells.

To quantify interleukin-36 receptor antagonist (IL-36Ra), interleukin-37 (IL-37), and interleukin-38 (IL-38) levels in aqueous humor and plasma of patients with primary glaucoma, and investigate their correlations with glaucomatous structural damage.

Anti-Inflammatory Cytokine Signatures in Primary Glaucoma Subtypes: Diagnostic and Pathophysiological Insights From IL-36Ra, IL-37, and IL-38.

Chronic stress potentially accelerates aging and increases age-related disease risk. However, the underlying mechanisms and organ specificity remain unclear. Utilizing a 12-month unpredictable chronic mild stress (UCMS) mouse model, we revealed chronic stress significantly exacerbates systemic inflammation, causing widespread tissue damage and lifespan shortening. Integrated single-cell RNA sequencing analysis of multiple tissues suggests a common feature of lymphocyte reduction and myeloid cell expansion. The lungs and kidneys exhibited pronounced stress responses, including epigenetic dysregulation and mitochondrial dysfunction. Both β-nicotinamide mononucleotide (NMN) and compound medicine Bazi Bushen (BZBS) effectively alleviate the UCMS-induced aging phenotypes. BZBS restores immune system homeostasis and reduces stress/inflammation levels in various tissues. Bioactive compounds from BZBS constituentsimproved mitochondrial function via AMPK-SIRT-PGC-1α signaling activation. The present study underscores the importance of early intervention in chronic stress to prevent or delay age-related diseases, with significant implications for public health strategies aimed at healthy aging.

Article	Year
Bazi Bushen mitigates epigenetic aging and extends healthspan in naturally aging mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie\| PubMed ID: 36764132	2023
Bazi Bushen ameliorates age-related energy metabolism dysregulation by targeting the IL-17/TNF inflammatory pathway associated with SASP. Chinese medicine\| PubMed ID: 38594761	2024
Characteristics of optic disc hemorrhage and optic nerve changes following acute primary angle closure. Frontiers in neurology\| PubMed ID: 38854957	2024
Targeting LKB1-AMPK-SIRT1-induced autophagy and mitophagy pathways improves cerebrovascular homeostasis in APP/PS1 mice. Free radical biology & medicine\| PubMed ID: 40180019	2025
Intervention of Bazi Bushen Capsule on Benign Prostatic Hyperplasia Concomitant with Erectile Dysfunction Induced by Testosterone Supplementation in Spontaneously Hypertensive Rats. Chinese journal of integrative medicine\| PubMed ID: 40419843	2025
Two-Year Outcomes of Goniotomy After Failed Surgery for Glaucoma: A Multicenter Study. Ophthalmology science\| PubMed ID: 41049109	2026
Bazi Bushen capsule alleviates skin photoaging by targeting the KEAP1-NRF2 pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology\| PubMed ID: 41101077	2025
Hyperoside Modulates the Estrogen-PI3K/VEGF Axis to Ameliorate Oxidative Damage-Induced Inhibition of Bone Formation in MC3T3-E1 Cells. Immunity, inflammation and disease\| PubMed ID: 41943235	2026
Anti-Inflammatory Cytokine Signatures in Primary Glaucoma Subtypes: Diagnostic and Pathophysiological Insights From IL-36Ra, IL-37, and IL-38. Journal of ophthalmology\| PubMed ID: 42344788	2026
Reversal of Chronic Stress-Induced Inflammaging by Bazi Bushen Formula Through AMPK-Sirtuins Axis Activation and Myeloid Reset. Exploration (Beijing, China)\| PubMed ID: 42861278	2026

Yawen Li

Biography

JoVE Journal Publications

Other Publications