long-non-coding-rna-h19-protection-against-myocardial

Long Non-Coding RNA H19 Protection Against Myocardial Ischemia/Reperfusion Injury in Diabetic Mice Through Hypoxia-Inducible Factor 1 Alpha Signaling

Diabetes can aggravate myocardial ischemia/reperfusion injury (MIRI), but the role of long non-coding RNA H19 (lncRNA H19) is still unclear. The purpose ...

Nanchang University

the second affiliated hospital of nanchang university

Neuropathic pain caused by nervous system damage or system dysfunction. The pathogenesis and the mechanism underlying neuropathic pain remains unclear. The only known neurobiological component involved in the neuropathic pain is nitric oxide (NO). NO is synthesized by nitric oxide synthase (nNOS) from L-arginine and oxygen. nNOS is involved in the inflammatory pain and neuropathic pain. In this study, we aimed to identify whether KN93 reduced the pain in the rats. Sixty adult male SD rat were randomly divided into 4 groups. Sham group and model group were not received treatment. Experimental group received intrathecal injection of KN93, and negative control group received DMSO injection 30 min before pain test. After last test of pain threshold, the rats were sacrificed and lumbar spinal tissues were sampled for analysis of the expression of pnNOS and pCaMK II by quantitative PCR and Western blotting. Pain threshold was increased in the rats received KN93 treatment (P<0.01), and the expression levels of pnNOS was increased (P<0.05) in experimental group and accompanied with decrease of CaMK II expression (P<0.05). By administration of KN93, the interaction of nNOS and the adaptor protein CAPON was reduced through inhibition of CaMK II by KN93. In conclusion, this study reveals that KN93 can reduce neuropathic pain via inhibiting the activity of CaMK II, and then increase the level of phosphorylated nNOS, to reduce the interaction with CAPON.

Phosphorylated neuronal nitric oxide synthase in neuropathic pain in rats.

Horseshoe kidney deformity with duplex urinary collecting systems is a rare congenital urinary tract defect. Clinically, it is very difficult to visually observe and examine the whole anatomic structure and information with the regular 2D diagnostic imaging tools. Here, we report a case in which a middle age patient has bilateral duplex urinary collecting systems and horseshoe kidney deformity accompanied with right renal ureteral calculi and hydronephrosis. It was diagnosed by magnetic resonance urography with urinary system 3D reconstruction. The imaging and display method provides valuable information about abnormal anatomic structures of the kidneys and the related stone diseases for preoperative planning.

Bilateral duplex urinary collecting systems accompanied with horseshoe kidneys deformity and right renal ureteral calculi and hydronephrosis: Diagnosis in magnetic resonance urography.

A stellate ganglion block (SGB) is a clinical sympathetic block which can inhibit the body systemic inflammatory response. However, whether and how SGB can attenuate the sepsis-induced acute lung injury remains unclear. Here, we evaluated the effect of SGB on sepsis-induced acute lung injury in rats. Ninety healthy Sprague Dawley (SD) male rats were divided into three groups: the sham operation group (S group), sepsis group (Sep group), and SGB group. The sepsis model rats were produced by cecum ligation and puncture (CLP), and blood samples were taken from the abdominal aorta of the rats at different time points for evaluating the concentration of TNF-α, IL-6, and IL-10 by enzyme-linked immunosorbent assay (ELISA). The rats were sacrificed, and lungs were collected to measure the wet/dry (W/D) lung tissue weight ratio, score the lung tissue pathological damage by microscopic examination, determine the myeloperoxidase (MPO) activity by spectrophotometry, and measure nuclear factor-kappa B (NF-κB) p65 expression by Western blot. The concentration of serum TNF-α, IL-6, and IL-10, lung tissue W/D ratio, pathological injury score, MPO activity, and expression of NF-κB p65 were higher in the Sep group compared with the S group at T. Furthermore, the concentration of serum TNF-α and IL-6, lung tissue W/D ratio, pathological damage score, MPO activity, and expression of NF-κB p65 were reduced and the concentration of IL-10 was increased in the SGB group compared with the Sep group at T. The successful sepsis model rats were induced by CLP, and SGB attenuated the sepsis-induced acute lung injury in rats.

Effect of a Stellate Ganglion Block on Acute Lung Injury in Septic Rats.

Exposure of neonatal animals to anesthetics may cause developmental functional changes and acute structural anomalies in the brain. Clonidine, an α2-adrenoceptor agonist, functions as an analgesic and sedative and protects against brain injury. Nevertheless, whether clonidine protects the developing brain from damage caused by sevoflurane (SEVO) anesthesia remains unclear. Seven-day-old rats were exposed to 3% SEVO for 6 h, during which time either clonidine or saline was injected three times. The arterial blood gases, respiratory rate, and anesthesia level of each rat were evaluated. Western blot analysis was employed to detect proinflammatory cytokines, NF-κB, and cleaved-caspase-3. Surgical anesthesia was adequately induced by SEVO. No rats died during the study. Compared with untreated rats, SEVO induced production of cleaved-caspase-3. Administration of clonidine and SEVO significantly reduced apoptosis. Moreover, nuclear translocation and NF-κB phosphorylation were inhibited by clonidine, and interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) production decreased after SEVO administration. Marked apoptosis in the brain was induced by SEVO anesthesia. Clonidine treatment provided significant protection against SEVO-induced apoptosis.

Clonidine Protects Against Neurotoxicity Induced by Sevoflurane Through NF-κB Signaling Inhibition and Proinflammatory Cytokine Release in Rats.

The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression.

MicroRNA-132 suppresses migration and invasion of renal carcinoma cells.

To make a new simple respirator and observe the oxygen therapy effect of the respirator on patients with severe and critical coronavirus disease 2019 (COVID-19).

[Effect of new simple breathing apparatus on oxygen therapy in patients with severe and critical coronavirus disease 2019].

To evaluate the efficacy and safety of SHR4640, a highly selective urate transporter 1 inhibitor, in Chinese subjects with hyperuricaemia.

Efficacy and safety of a selective URAT1 inhibitor SHR4640 in Chinese subjects with hyperuricaemia: a randomized controlled phase II study.

The objective of this study was to investigate the involvement of the long non-coding RNA (lncRNA) BRE-AS1 in clear cell renal cell carcinoma (ccRCC) and to explore its potential therapeutic role.

Long non-coding RNA BRE-AS1 inhibits proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating miR-106b-5p.

Sevoflurane's potential impact on cognitive function and neurodevelopment, especially in susceptible populations such as infants and the elderly, has raised widespread concern. This study focuses on how sevoflurane induces ferroptosis in astrocytes and identifies solute carrier family 7 member 11 (SLC7A11) as a mediator of ferroptosis, providing new insights into sevoflurane-related neurotoxic pathways. We analysed single-cell sequencing (scRNA-seq) data from sevoflurane-exposed mice and control mice, supplemented with bulk RNA-seq data, to assess gene expression alterations. Additionally, pregnant mice were subjected to in vivo experiments, and in vitro studies using U251 astrocytoma cells were conducted to evaluate sevoflurane's neurotoxic effects on offspring, focusing on ferroptosis markers and SLC7A11 expression. Sevoflurane exposure led to learning, memory and behavioural deficits in offspring, associated with decreased SLC7A11 expression and increased signs of ferroptosis. In U251 cells, sevoflurane reduced cell viability, increased reactive oxygen species (ROS) levels and affected the expression of ferroptosis regulatory factors, supporting the hypothesis that sevoflurane induces astrocyte ferroptosis through SLC7A11 modulation. Molecular docking experiments suggest a direct interaction between sevoflurane and SLC7A11. This study provides mechanistic insights into sevoflurane-induced neurotoxicity, emphasising the importance of SLC7A11 in regulating astrocyte ferroptosis. Our findings highlight the potential for targeting ferroptosis pathways to mitigate the adverse effects of sevoflurane anaesthesia.

Comprehensive Analysis of Bulk RNA-Seq and Single-Cell RNA-Seq Data Unveils Sevoflurane-Induced Neurotoxicity Through SLC7A11-Associated Ferroptosis.

Oral microbiome homeostasis is important for children's health, and microbial community is affected by anesthetics. The application of anesthetics in children's oral therapy has become a relatively mature method. This study aims to investigate the effect of different anesthesia techniques on children's oral microbiota.

Article	Year
Phosphorylated neuronal nitric oxide synthase in neuropathic pain in rats. International journal of clinical and experimental pathology\| PubMed ID: 26722464	2015
Bilateral duplex urinary collecting systems accompanied with horseshoe kidneys deformity and right renal ureteral calculi and hydronephrosis: Diagnosis in magnetic resonance urography. Journal of X-ray science and technology\| PubMed ID: 28234270	2017
Effect of a Stellate Ganglion Block on Acute Lung Injury in Septic Rats. Inflammation\| PubMed ID: 29737475	2018
Clonidine Protects Against Neurotoxicity Induced by Sevoflurane Through NF-κB Signaling Inhibition and Proinflammatory Cytokine Release in Rats. Journal of molecular neuroscience : MN\| PubMed ID: 30073555	2018
MicroRNA-132 suppresses migration and invasion of renal carcinoma cells. Journal of clinical laboratory analysis\| PubMed ID: 31625200	2020
[Effect of new simple breathing apparatus on oxygen therapy in patients with severe and critical coronavirus disease 2019]. Zhonghua wei zhong bing ji jiu yi xue\| PubMed ID: 32788025	2020
Efficacy and safety of a selective URAT1 inhibitor SHR4640 in Chinese subjects with hyperuricaemia: a randomized controlled phase II study. Rheumatology (Oxford, England)\| PubMed ID: 33693494	2021
Long non-coding RNA BRE-AS1 inhibits proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating miR-106b-5p. Histology and histopathology\| PubMed ID: 37530129	2024
Comprehensive Analysis of Bulk RNA-Seq and Single-Cell RNA-Seq Data Unveils Sevoflurane-Induced Neurotoxicity Through SLC7A11-Associated Ferroptosis. Journal of cellular and molecular medicine\| PubMed ID: 39724413	2024
Anesthetics change the oral microbial composition of children and increase the abundance of the genus . Translational pediatrics\| PubMed ID: 39823002	2024

Lian Guo

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