generation-novel-dendritic-cell-vaccine-using-melanoma-squamous

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

We identified cancer stem cell (CSC)-enriched populations from murine melanoma D5 syngeneic to C57BL/6 mice and the squamous cancer SCC7 syngeneic to C3H ...

University of Michigan

to design in vitro and in vivo models of metastasis to study the behavior of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC).

Metastatic potential of cancer stem cells in head and neck squamous cell carcinoma.

&#xC554; &#xC904;&#xAE30; &#xC138;&#xD3EC; (CSC) &#xC774;&#xB860; &#xACB0;&#xB860; subpopulation &#xC554; &#xC138;&#xD3EC;, &#xC554; &#xC904;&#xAE30; &#xC138;&#xD3EC;&#xC758; &#xC218; self-renew &#xD558; &#xACE0; &#xC885;&#xC591;&#xC758; &#xC131;&#xC7A5;&#xC5D0; &#xB300; &#xD55C; &#xCC45;&#xC784;&#xC774; &#xC788;&#xC2B5;&#xB2C8;&#xB2E4;. &#xC774;&#xC804; &#xC5F0;&#xAD6C; &#xCE21;&#xBA74; &#xC778;&#xAD6C; (SP)&#xC73C;&#xB85C; &#xC140; &#xC218; &#xACBD;&#xACFC; Hoechst 33342 &#xC5FC;&#xB8CC;&#xB97C; &#xD655;&#xC778; &#xD588;&#xB2E4;. SP &#xC140; &#xACF5;&#xC720; &#xB9CE;&#xC740; &#xD2B9;&#xC131;, CSCs &#xC545;&#xC131; &#xC885;&#xC591;&#xC5D0;&#xC11C; &#xACE0;&#xB9BD; &#xB41C; SP &#xD3EC;&#xD568; CSCs &#xC81C;&#xC548;.

&#xBA38;&#xB9AC;&#xC640; &#xBAA9; &#xC554; &#xC904;&#xAE30; &#xC138;&#xD3EC;: &#xC0AC;&#xC774;&#xB4DC; &#xC778;&#xAD6C;.

La teor&iacute;a de la c&eacute;lula de v&aacute;stago (CSC) de c&aacute;ncer concluye que una subpoblaci&oacute;n de c&eacute;lulas cancerosas, las c&eacute;lulas de c&aacute;ncer, pueden autorrenovarse y son responsable del crecimiento del tumor. Estudios previos han identificado c&eacute;lulas capaces de eflujo Hoechst 33342 tinte como la poblaci&oacute;n de lado (SP). SP c&eacute;lulas y CSC comparte muchas caracter&iacute;sticas, sugiriendo el SP aislado de tumores malignos contiene CSC.

Cabeza y cuello de c&eacute;lulas madre del c&aacute;ncer: la poblaci&oacute;n de lado.

&#x764C;&#x75C7;&#x5E72;&#x7EC6;&#x80DE; (CSC) &#x7406;&#x8BBA;&#x7684;&#x7ED3;&#x8BBA;&#x4E9A;&#x7FA4;&#x7684;&#x80BF;&#x7624;&#x7EC6;&#x80DE;&#xFF0C;&#x80BF;&#x7624;&#x5E72;&#x7EC6;&#x80DE;&#xFF0C;&#x53EF;&#x4EE5; self-renew &#x8D1F;&#x8D23;&#x5BF9;&#x80BF;&#x7624;&#x7684;&#x751F;&#x957F;&#x3002;&#x4EE5;&#x524D;&#x7684;&#x7814;&#x7A76;&#x5DF2;&#x786E;&#x5B9A;&#x7EC6;&#x80DE;&#x80FD;&#x591F;&#x5C04;&#x6D41; Hoechst 33342 &#x67D3;&#x6599;&#x4E3A;&#x4FA7;&#x4EBA;&#x53E3; (SP)&#x3002;SP &#x7EC6;&#x80DE;&#x548C;&#x5C0F;&#x533A;&#x5171;&#x4EAB;&#x8BB8;&#x591A;&#x7279;&#x6027;&#xFF0C;&#xFF0C;&#x8868;&#x660E;&#x5206;&#x79BB;&#x682A;&#x6076;&#x6027;&#x9AA8;&#x80BF;&#x7624;&#x7684; SP &#x5305;&#x542B;&#x5C0F;&#x533A;&#x3002;

&#x5934;&#x9888;&#x90E8;&#x80BF;&#x7624;&#x5E72;&#x7EC6;&#x80DE;&#xFF1A; &#x4FA7;&#x4EBA;&#x53E3;&#x3002;

The cancer stem cell (CSC) theory concludes that a subpopulation of cancer cells, the cancer stem cells, can self-renew and are responsible for tumor growth. Previous studies have identified cells able to efflux Hoechst 33342 dye as the side population (SP). SP cells and CSCs share many characteristics, suggesting the SP isolated from malignant tumors contains CSCs.

Head and neck cancer stem cells: the side population.

La th&eacute;orie de cellules souches (CSC) du cancer conclut qu&#39;une sous-population de cellules canc&eacute;reuses, les cellules souches canc&eacute;reuses, pouvez auto-renouveler et sont responsable de la croissance tumorale. Des &eacute;tudes ant&eacute;rieures ont identifi&eacute; des cellules capables d&#39;efflux de Hoechst 33342 colorant comme la population de c&ocirc;t&eacute; (SP). SP cellules et CCS partagent plusieurs caract&eacute;ristiques, sugg&eacute;rant la SP isol&eacute;e de tumeurs malignes contient CSCs.

La t&ecirc;te et le cou des cellules souches canc&eacute;reuses : la population de c&ocirc;t&eacute;.

Die Krebs-Stammzellen (CSC)-Theorie stellt fest, dass eine Subpopulation von Krebszellen, die Krebsstammzellen erneuern k&ouml;nnen und sind verantwortlich f&uuml;r Tumorwachstum. Fr&uuml;here Studien haben Zellen in der Lage, Efflux Hoechst 33342 Farbstoff als die Seite Bev&ouml;lkerung (SP) identifiziert. SP haben Zellen und CSCs viele Gemeinsamkeiten, was darauf hindeutet, dass der SP isoliert von malignen Tumoren CSCs enth&auml;lt.

Kopf und Hals Krebsstammzellen: Seite Bev&ouml;lkerung.

La teoria di cancro cellule staminali (CSC) conclude che una sottopopolazione di cellule tumorali, le cellule staminali del cancro, pu&ograve; auto-rinnovarsi e sono responsabile della crescita tumorale. Precedenti studi hanno identificato le cellule in grado di efflusso colorante Hoechst 33342 come la popolazione di lato (SP). SP, celle e CSCs condividono molte caratteristiche, suggerendo la SP isolato da tumori maligni contiene CSCs.

Testa e del collo cancro cellule staminali: la popolazione di lato.

&#x304C;&#x3093;&#x5E79;&#x7D30;&#x80DE; &#xFF08;CSC&#xFF09; &#x306E;&#x7406;&#x8AD6;&#x306F;&#x3001;&#x304C;&#x3093;&#x7D30;&#x80DE;&#x3001;&#x304C;&#x3093;&#x5E79;&#x7D30;&#x80DE;&#x306E;&#x4E9C;&#x96C6;&#x56E3;&#x304C;&#x81EA;&#x5DF1;&#x66F4;&#x65B0;&#x3059;&#x308B;&#x3053;&#x3068;&#x304C;&#x3067;&#x304D;&#x3001;&#x816B;&#x760D;&#x306E;&#x6210;&#x9577;&#x306B;&#x8CAC;&#x4EFB;&#x304C;&#x3042;&#x308B;&#x7D42;&#x308F;&#x308A;&#x307E;&#x3059;&#x3002;&#x4EE5;&#x524D;&#x306E;&#x7814;&#x7A76;&#x30D8;&#x30AD;&#x30B9;&#x30C8; 33342 &#x67D3;&#x6599;&#x306E;&#x6D41;&#x51FA;&#x3059;&#x308B;&#x3053;&#x3068;&#x304C;&#x3067;&#x304D;&#x308B;&#x7D30;&#x80DE;&#x5074;&#x306E;&#x4EBA;&#x53E3; (SP) &#x3068;&#x3057;&#x3066;&#x8B58;&#x5225;&#x3057;&#x307E;&#x3057;&#x305F;&#x3002;SP &#x7D30;&#x80DE;&#x3068; CSCs &#x591A;&#x304F;&#x7279;&#x6027; CSCs &#x60AA;&#x6027;&#x816B;&#x760D;&#x304B;&#x3089;&#x5206;&#x96E2;&#x3055;&#x308C;&#x305F; SP &#x3092;&#x683C;&#x7D0D;&#x793A;&#x5506;&#x3057;&#x3066;&#x5171;&#x6709;&#x3057;&#x307E;&#x3059;&#x3002;

&#x982D;&#x9838;&#x90E8;&#x304C;&#x3093;&#x5E79;&#x7D30;&#x80DE;&#xFF1A; &#x5074;&#x306E;&#x4EBA;&#x53E3;&#x3002;

Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan-squamous cell carcinoma-104 (UM-SCC-104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs).

UM-SCC-104: a new human papillomavirus-16-positive cancer stem cell-containing head and neck squamous cell carcinoma cell line.

To evaluate in vitro the potential links between sialyl Lewis X (sLeX) and cancer stem cells (CSC) in head and neck squamous cell carcinoma (HNSCC). HNSCC is an aggressive malignancy with high mortality mainly due to metastasis. CSC have emerged as important players in HNSCC metastasis. sLeX is a tetrasaccharide carbohydrate known to play a key role in metastatic dissemination by promoting binding of the tumor cells to the endothelium.

In vitro evaluation of sialyl Lewis X relationship with head and neck cancer stem cells.

To determine if the behavior of cancer stem cells (CSCs) is affected by human papillomavirus (HPV) status.

Article	Year
Metastatic potential of cancer stem cells in head and neck squamous cell carcinoma. Archives of otolaryngology--head & neck surgery\| PubMed ID: 21173377	2010
Head and neck cancer stem cells: the side population. The Laryngoscope\| PubMed ID: 21344428	2011
UM-SCC-104: a new human papillomavirus-16-positive cancer stem cell-containing head and neck squamous cell carcinoma cell line. Head & neck\| PubMed ID: 22162267	2012
In vitro evaluation of sialyl Lewis X relationship with head and neck cancer stem cells. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery\| PubMed ID: 23558285	2013
Head and neck cancer stem cells: the effect of HPV--an in vitro and mouse study. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery\| PubMed ID: 23585151	2013

John H. Owen

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