Luís Pereira de Almeida CNC-UC - Center for Neuroscience and Cell Biology University of Coimbra Biography Publications Institution JoVE Articles Luís Pereira de Almeida has not added a biography. If you are Luís Pereira de Almeida and would like to personalize this page please email our Author Liaison for assistance. Publications Modeling Lysosomal Storage Disorders in an Innovative Way: Establishment and Characterization of Stem Cell Lines from Human Exfoliated Deciduous Teeth of Mucopolysaccharidosis Type II Patients International Journal of Molecular Sciences. Mar, 2024 | Pubmed ID: 38542525 Blood and Cerebellar Abundance of ATXN3 Splice Variants in Spinocerebellar Ataxia Type 3/Machado-Joseph Disease Neurobiology of Disease. Apr, 2024 | Pubmed ID: 38423193 Phosphoproteome Microarray Analysis of Extracellular Particles As a Tool to Explore Novel Biomarker Candidates for Alzheimer's Disease International Journal of Molecular Sciences. Jan, 2024 | Pubmed ID: 38338863 Graft-derived Neurons and Bystander Effects Are Maintained for Six Months After Human IPSC-derived NESC Transplantation in Mice's Cerebella Scientific Reports. Feb, 2024 | Pubmed ID: 38332227 Viral-based Animal Models in Polyglutamine Disorders Brain : a Journal of Neurology. Apr, 2024 | Pubmed ID: 38284949 RNA-based Liposomes for Oral Cancer: From Biophysical Characterization to Biological Evaluation International Journal of Biological Macromolecules. Feb, 2024 | Pubmed ID: 38199539 Correction To: The Frequency of Non‑motor Symptoms in SCA3 and Their Association with Disease Severity and Lifestyle Factors Journal of Neurology. Jan, 2024 | Pubmed ID: 37979094 Stage-Dependent Biomarker Changes in Spinocerebellar Ataxia Type 3 Annals of Neurology. Feb, 2024 | Pubmed ID: 37962377 Ghrelin Delays Premature Aging in Hutchinson-Gilford Progeria Syndrome Aging Cell. Dec, 2023 | Pubmed ID: 37858983 Selective, Broad-spectrum Antiviral Photodynamic Disinfection with Dicationic Imidazolyl Chlorin Photosensitizers Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology. Nov, 2023 | Pubmed ID: 37755667 A Novel Genetic Variant in Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia International Journal of Molecular Sciences. Aug, 2023 | Pubmed ID: 37628781 Evaluation of Biphasic Calcium Phosphate Scaffolds Derived from Cuttlefish Bone Coated with Poly(ester Urea) for Bone Tissue Regeneration Polymers. May, 2023 | Pubmed ID: 37242831 Extracellular Communication Between Brain Cells Through Functional Transfer of Cre MRNA Mediated by Extracellular Vesicles Molecular Therapy : the Journal of the American Society of Gene Therapy. Jul, 2023 | Pubmed ID: 37194237 Stage-dependent Biomarker Changes in Spinocerebellar Ataxia Type 3 MedRxiv : the Preprint Server for Health Sciences. Apr, 2023 | Pubmed ID: 37163081 Blood Transcriptome Sequencing Identifies Biomarkers Able to Track Disease Stages in Spinocerebellar Ataxia Type 3 Brain : a Journal of Neurology. Oct, 2023 | Pubmed ID: 37071051 Extracellular Vesicle-based Delivery of Silencing Sequences for the Treatment of Machado-Joseph Disease/spinocerebellar Ataxia Type 3 Molecular Therapy : the Journal of the American Society of Gene Therapy. May, 2023 | Pubmed ID: 37025062 Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography Versus Ultracentrifugation Biomolecules. Feb, 2023 | Pubmed ID: 36830647 Extracellular Communication Between Brain Cells Through Functional Transfer of Cre MRNA BioRxiv : the Preprint Server for Biology. Jan, 2023 | Pubmed ID: 36811091 A Standardised Protocol for Blood and Cerebrospinal Fluid Collection and Processing for Biomarker Research in Ataxia Neuropathology and Applied Neurobiology. Apr, 2023 | Pubmed ID: 36798010 The Stress Granule Protein G3BP1 Alleviates Spinocerebellar Ataxia-associated Deficits Brain : a Journal of Neurology. Jun, 2023 | Pubmed ID: 36511898 The Frequency of Non-motor Symptoms in SCA3 and Their Association with Disease Severity and Lifestyle Factors Journal of Neurology. Feb, 2023 | Pubmed ID: 36324033 Ataxin-2 in the Hypothalamus at the Crossroads Between Metabolism and Clock Genes Journal of Molecular Endocrinology. Jan, 2023 | Pubmed ID: 36103139 Editorial: Gene Therapy 2.0: Biotechnology for Circuit Engineering and Complex Therapeutic Approaches Frontiers in Bioengineering and Biotechnology. 2022 | Pubmed ID: 36483777 A New Protocol for Whole-brain Biodistribution Analysis of AAVs by Tissue Clearing, Light-sheet Microscopy and Semi-automated Spatial Quantification Gene Therapy. Dec, 2022 | Pubmed ID: 36316447 SIRT1 Activation and Its Circadian Clock Control: a Promising Approach Against (frailty In) Neurodegenerative Disorders Aging Clinical and Experimental Research. Dec, 2022 | Pubmed ID: 36306110 Establishment and Characterization of Human Pluripotent Stem Cells-derived Brain Organoids to Model Cerebellar Diseases Scientific Reports. Jul, 2022 | Pubmed ID: 35869235 Differential Temporal Dynamics of Axial and Appendicular Ataxia in SCA3 Movement Disorders : Official Journal of the Movement Disorder Society. Sep, 2022 | Pubmed ID: 35808813 SIRT2 Deficiency Exacerbates Hepatic Steatosis Via a Putative Role of the ER Stress Pathway International Journal of Molecular Sciences. Jun, 2022 | Pubmed ID: 35743232 Tau and Neurofilament Light-chain As Fluid Biomarkers in Spinocerebellar Ataxia Type 3 European Journal of Neurology. Aug, 2022 | Pubmed ID: 35478426 Cerebellar Morphometric and Spectroscopic Biomarkers for Machado-Joseph Disease Acta Neuropathologica Communications. Mar, 2022 | Pubmed ID: 35305685 Photodynamic Disinfection of SARS-CoV-2 Clinical Samples Using a Methylene Blue Formulation Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology. Jun, 2022 | Pubmed ID: 35304729 Using Genetically Modified Extracellular Vesicles As a Non-invasive Strategy to Evaluate Brain-specific Cargo Biomaterials. Feb, 2022 | Pubmed ID: 35033904 MiRNA-Mediated Knockdown of ATXN3 Alleviates Molecular Disease Hallmarks in a Mouse Model for Spinocerebellar Ataxia Type 3 Nucleic Acid Therapeutics. Jun, 2022 | Pubmed ID: 34878314 Characterization of Lifestyle in Spinocerebellar Ataxia Type 3 and Association with Disease Severity Movement Disorders : Official Journal of the Movement Disorder Society. Feb, 2022 | Pubmed ID: 34713931 The Autophagy-enhancing Drug Carbamazepine Improves Neuropathology and Motor Impairment in Mouse Models of Machado-Joseph Disease Neuropathology and Applied Neurobiology. Feb, 2022 | Pubmed ID: 34432315 ULK Overexpression Mitigates Motor Deficits and Neuropathology in Mouse Models of Machado-Joseph Disease Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2022 | Pubmed ID: 34298131 Identification of the Calpain-generated Toxic Fragment of Ataxin-3 Protein Provides New Avenues for Therapy of Machado-Joseph Disease| Spinocerebellar Ataxia Type 3 Neuropathology and Applied Neurobiology. Feb, 2022 | Pubmed ID: 34273111 Autophagy in Spinocerebellar Ataxia Type 2, a Dysregulated Pathway, and a Target for Therapy Cell Death & Disease. Nov, 2021 | Pubmed ID: 34845184 Extracellular Vesicles Physiological Role and the Particular Case of Disease-Spreading Mechanisms in Polyglutamine Diseases International Journal of Molecular Sciences. Nov, 2021 | Pubmed ID: 34830171 The Parkinson's-disease-associated Mutation LRRK2-G2019S Alters Dopaminergic Differentiation Dynamics Via NR2F1 Cell Reports. Oct, 2021 | Pubmed ID: 34686322 Highly Specific Blood-Brain Barrier Transmigrating Single-Domain Antibodies Selected by an In Vivo Phage Display Screening Pharmaceutics. Oct, 2021 | Pubmed ID: 34683891 Polyglutamine-Expanded Ataxin-3: A Target Engagement Marker for Spinocerebellar Ataxia Type 3 in Peripheral Blood Movement Disorders : Official Journal of the Movement Disorder Society. Nov, 2021 | Pubmed ID: 34397117 Living Proof of Activity of Extracellular Vesicles in the Central Nervous System International Journal of Molecular Sciences. Jul, 2021 | Pubmed ID: 34298912 Stress Granules, RNA-binding Proteins and Polyglutamine Diseases: Too Much Aggregation? Cell Death & Disease. Jun, 2021 | Pubmed ID: 34103467 Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (4th Edition) Autophagy. Feb, 2021 | Pubmed ID: 33634751 Neuropeptide Y (NPY) Intranasal Delivery Alleviates Machado-Joseph Disease Scientific Reports. Feb, 2021 | Pubmed ID: 33558582 Mesenchymal Stromal Cells to Fight SARS-CoV-2: Taking Advantage of a Pleiotropic Therapy Cytokine & Growth Factor Reviews. Apr, 2021 | Pubmed ID: 33397585 Mesenchymal Stromal Cells' Therapy for Polyglutamine Disorders: Where Do We Stand and Where Should We Go? Frontiers in Cellular Neuroscience. 2020 | Pubmed ID: 33132851 Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellular Vesicles for Transcriptional Research Molecular Therapy. Methods & Clinical Development. Sep, 2020 | Pubmed ID: 32913880 The Blood-brain Barrier is Disrupted in Machado-Joseph Disease/spinocerebellar Ataxia Type 3: Evidence from Transgenic Mice and Human Post-mortem Samples Acta Neuropathologica Communications. Aug, 2020 | Pubmed ID: 32867861 Neurofilaments in Spinocerebellar Ataxia Type 3: Blood Biomarkers at the Preataxic and Ataxic Stage in Humans and Mice EMBO Molecular Medicine. Jul, 2020 | Pubmed ID: 32510847 Trehalose Alleviates the Phenotype of Machado-Joseph Disease Mouse Models Journal of Translational Medicine. Apr, 2020 | Pubmed ID: 32272938 Protocol for the Characterization of the Cytosine-Adenine-Guanine Tract and Flanking Polymorphisms in Machado-Joseph Disease: Impact on Diagnosis and Development of Gene-Based Therapies The Journal of Molecular Diagnostics : JMD. Jun, 2020 | Pubmed ID: 32205289 MiRNA-31 Improves Cognition and Abolishes Amyloid-β Pathology by Targeting APP and BACE1 in an Animal Model of Alzheimer's Disease Molecular Therapy. Nucleic Acids. Mar, 2020 | Pubmed ID: 32069773 Neuropeptide Y Enhances Progerin Clearance and Ameliorates the Senescent Phenotype of Human Hutchinson-Gilford Progeria Syndrome Cells The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. May, 2020 | Pubmed ID: 32012215 Antisense Oligonucleotide Therapeutics in Neurodegenerative Diseases: the Case of Polyglutamine Disorders Brain : a Journal of Neurology. Feb, 2020 | Pubmed ID: 31738395 Successes and Hurdles in Stem Cells Application and Production for Brain Transplantation Frontiers in Neuroscience. 2019 | Pubmed ID: 31802998 Restoring Brain Cholesterol Turnover Improves Autophagy and Has Therapeutic Potential in Mouse Models of Spinocerebellar Ataxia Acta Neuropathologica. Nov, 2019 | Pubmed ID: 31197505 Ibuprofen Enhances Synaptic Function and Neural Progenitors Proliferation Markers and Improves Neuropathology and Motor Coordination in Machado-Joseph Disease Models Human Molecular Genetics. Nov, 2019 | Pubmed ID: 31127937 Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality Stem Cell Reports. May, 2019 | Pubmed ID: 30982740 RNA Interference Therapy for Machado-Joseph Disease: Long-Term Safety Profile of Lentiviral Vectors Encoding Short Hairpin RNAs Targeting Mutant Ataxin-3 Human Gene Therapy. Jul, 2019 | Pubmed ID: 30760052 Cordycepin Activates Autophagy Through AMPK Phosphorylation to Reduce Abnormalities in Machado-Joseph Disease Models Human Molecular Genetics. Jan, 2019 | Pubmed ID: 30219871 Machado-Joseph Disease/spinocerebellar Ataxia Type 3: Lessons from Disease Pathogenesis and Clues into Therapy Journal of Neurochemistry. Jan, 2019 | Pubmed ID: 29959858 Loss of Hierarchical Imprinting Regulation at the Prader-Willi/Angelman Syndrome Locus in Human IPSCs Human Molecular Genetics. Dec, 2018 | Pubmed ID: 30102380 Repeated Mesenchymal Stromal Cell Treatment Sustainably Alleviates Machado-Joseph Disease Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2018 | Pubmed ID: 30087083 Generation and Characterization of a Human IPS Cell Line from a Patient-related Control to Study Disease Mechanisms Associated with DAND5 P.R152H Alteration Stem Cell Research. May, 2018 | Pubmed ID: 29730570 Stem Cell-Based Therapies for Polyglutamine Diseases Advances in Experimental Medicine and Biology. 2018 | Pubmed ID: 29427116 Gene Therapies for Polyglutamine Diseases Advances in Experimental Medicine and Biology. 2018 | Pubmed ID: 29427115 Molecular Mechanisms and Cellular Pathways Implicated in Machado-Joseph Disease Pathogenesis Advances in Experimental Medicine and Biology. 2018 | Pubmed ID: 29427113 Dipeptidyl Peptidase IV (DPP-IV) Inhibition Prevents Fibrosis in Adipose Tissue of Obese Mice Biochimica Et Biophysica Acta. General Subjects. Mar, 2018 | Pubmed ID: 29154902 Control of Huntington's Disease-Associated Phenotypes by the Striatum-Enriched Transcription Factor Foxp2 Cell Reports. Dec, 2017 | Pubmed ID: 29212017 Generation of Human IPSC Line from a Patient with Laterality Defects and Associated Congenital Heart Anomalies Carrying a DAND5 Missense Alteration Stem Cell Research. Dec, 2017 | Pubmed ID: 29136563 Extracellular Vesicles: Novel Promising Delivery Systems for Therapy of Brain Diseases Journal of Controlled Release : Official Journal of the Controlled Release Society. Sep, 2017 | Pubmed ID: 28687495 Unravelling Endogenous MicroRNA System Dysfunction As a New Pathophysiological Mechanism in Machado-Joseph Disease Molecular Therapy : the Journal of the American Society of Gene Therapy. Apr, 2017 | Pubmed ID: 28236575 Unraveling the Role of Ataxin-2 in Metabolism Trends in Endocrinology and Metabolism: TEM. Apr, 2017 | Pubmed ID: 28117213 Caffeine Alleviates Progressive Motor Deficits in a Transgenic Mouse Model of Spinocerebellar Ataxia Annals of Neurology. Mar, 2017 | Pubmed ID: 28032667 Proteolytic Cleavage of Polyglutamine Disease-Causing Proteins: Revisiting the Toxic Fragment Hypothesis Current Pharmaceutical Design. 2017 | Pubmed ID: 28025946 Motor Dysfunctions and Neuropathology in Mouse Models of Spinocerebellar Ataxia Type 2: A Comprehensive Review Frontiers in Neuroscience. 2016 | Pubmed ID: 28018166 Gene Therapy for the CNS Using AAVs: The Impact of Systemic Delivery by AAV9 Journal of Controlled Release : Official Journal of the Controlled Release Society. Nov, 2016 | Pubmed ID: 27637390 Neuropeptide Y (NPY) As a Therapeutic Target for Neurodegenerative Diseases Neurobiology of Disease. Nov, 2016 | Pubmed ID: 27461050 Caloric Restriction Stimulates Autophagy in Rat Cortical Neurons Through Neuropeptide Y and Ghrelin Receptors Activation Aging. Jul, 2016 | Pubmed ID: 27441412 Fibroblasts of Machado Joseph Disease Patients Reveal Autophagy Impairment Scientific Reports. Jun, 2016 | Pubmed ID: 27328712 Adenosine A2A Receptors in the Amygdala Control Synaptic Plasticity and Contextual Fear Memory Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology. Nov, 2016 | Pubmed ID: 27312408 Caloric Restriction Blocks Neuropathology and Motor Deficits in Machado-Joseph Disease Mouse Models Through SIRT1 Pathway Nature Communications. May, 2016 | Pubmed ID: 27165717 Safety Profile of the Intravenous Administration of Brain-targeted Stable Nucleic Acid Lipid Particles Data in Brief. Mar, 2016 | Pubmed ID: 26958628 Ataxin-3 Phosphorylation Decreases Neuronal Defects in Spinocerebellar Ataxia Type 3 Models The Journal of Cell Biology. Feb, 2016 | Pubmed ID: 26880203 Intravenous Administration of Brain-targeted Stable Nucleic Acid Lipid Particles Alleviates Machado-Joseph Disease Neurological Phenotype Biomaterials. Mar, 2016 | Pubmed ID: 26757259 Gephyrin Cleavage in In Vitro Brain Ischemia Decreases GABAA Receptor Clustering and Contributes to Neuronal Death Molecular Neurobiology. Aug, 2016 | Pubmed ID: 26093381 Re-establishing Ataxin-2 Downregulates Translation of Mutant Ataxin-3 and Alleviates Machado-Joseph Disease Brain : a Journal of Neurology. Dec, 2015 | Pubmed ID: 26490332 Neuropeptide Y Mitigates Neuropathology and Motor Deficits in Mouse Models of Machado-Joseph Disease Human Molecular Genetics. Oct, 2015 | Pubmed ID: 26220979 MiRNA-21 Silencing Mediated by Tumor-targeted Nanoparticles Combined with Sunitinib: A New Multimodal Gene Therapy Approach for Glioblastoma Journal of Controlled Release : Official Journal of the Controlled Release Society. Jun, 2015 | Pubmed ID: 25861727 Neuropeptide Y Stimulates Autophagy in Hypothalamic Neurons Proceedings of the National Academy of Sciences of the United States of America. Mar, 2015 | Pubmed ID: 25775546 Transplantation of Cerebellar Neural Stem Cells Improves Motor Coordination and Neuropathology in Machado-Joseph Disease Mice Brain : a Journal of Neurology. Feb, 2015 | Pubmed ID: 25527827 Let-7 Coordinately Suppresses Components of the Amino Acid Sensing Pathway to Repress MTORC1 and Induce Autophagy Cell Metabolism. Oct, 2014 | Pubmed ID: 25295787 RNA Interference Mitigates Motor and Neuropathological Deficits in a Cerebellar Mouse Model of Machado-Joseph Disease PloS One. 2014 | Pubmed ID: 25144231 Calpain Inhibition Reduces Ataxin-3 Cleavage Alleviating Neuropathology and Motor Impairments in Mouse Models of Machado-Joseph Disease Human Molecular Genetics. Sep, 2014 | Pubmed ID: 24817574 Fluoxetine Induces Proliferation and Inhibits Differentiation of Hypothalamic Neuroprogenitor Cells in Vitro PloS One. 2014 | Pubmed ID: 24598761 IGF-1 Intranasal Administration Rescues Huntington's Disease Phenotypes in YAC128 Mice Molecular Neurobiology. Jun, 2014 | Pubmed ID: 24347322 Role of Hypothalamic Neurogenesis in Feeding Regulation Trends in Endocrinology and Metabolism: TEM. Feb, 2014 | Pubmed ID: 24231724 Beclin 1 Mitigates Motor and Neuropathological Deficits in Genetic Mouse Models of Machado-Joseph Disease Brain : a Journal of Neurology. Jul, 2013 | Pubmed ID: 23801739 Tumor-targeted Chlorotoxin-coupled Nanoparticles for Nucleic Acid Delivery to Glioblastoma Cells: A Promising System for Glioblastoma Treatment Molecular Therapy. Nucleic Acids. Jun, 2013 | Pubmed ID: 23778499 Caffeine and Adenosine A(2A) Receptor Inactivation Decrease Striatal Neuropathology in a Lentiviral-based Model of Machado-Joseph Disease Annals of Neurology. May, 2013 | Pubmed ID: 23625556 Silencing Mutant Ataxin-3 Rescues Motor Deficits and Neuropathology in Machado-Joseph Disease Transgenic Mice PloS One. 2013 | Pubmed ID: 23349684 Overexpression of Mutant Ataxin-3 in Mouse Cerebellum Induces Ataxia and Cerebellar Neuropathology Cerebellum (London, England). Aug, 2013 | Pubmed ID: 23242710 MicroRNA-21 Silencing Enhances the Cytotoxic Effect of the Antiangiogenic Drug Sunitinib in Glioblastoma Human Molecular Genetics. Mar, 2013 | Pubmed ID: 23201752 PDGF-B-mediated Downregulation of MiR-21: New Insights into PDGF Signaling in Glioblastoma Human Molecular Genetics. Dec, 2012 | Pubmed ID: 22922228 Calpastatin-mediated Inhibition of Calpains in the Mouse Brain Prevents Mutant Ataxin 3 Proteolysis, Nuclear Localization and Aggregation, Relieving Machado-Joseph Disease Brain : a Journal of Neurology. Aug, 2012 | Pubmed ID: 22843411 MiR-155 Modulates Microglia-mediated Immune Response by Down-regulating SOCS-1 and Promoting Cytokine and Nitric Oxide Production Immunology. Jan, 2012 | Pubmed ID: 22043967 Gene Therapy for Parkinson's and Alzheimer's Diseases: from the Bench to Clinical Trials Current Pharmaceutical Design. 2011 | Pubmed ID: 21902665 Moderate Long-term Modulation of Neuropeptide Y in Hypothalamic Arcuate Nucleus Induces Energy Balance Alterations in Adult Rats PloS One. 2011 | Pubmed ID: 21799827 Proliferative Hypothalamic Neurospheres Express NPY, AGRP, POMC, CART and Orexin-A and Differentiate to Functional Neurons PloS One. May, 2011 | Pubmed ID: 21589937 Overexpression of the Autophagic Beclin-1 Protein Clears Mutant Ataxin-3 and Alleviates Machado-Joseph Disease Brain : a Journal of Neurology. May, 2011 | Pubmed ID: 21478185 Silencing Ataxin-3 Mitigates Degeneration in a Rat Model of Machado-Joseph Disease: No Role for Wild-type Ataxin-3? Human Molecular Genetics. Jun, 2010 | Pubmed ID: 20308049 Allele-specific RNA Silencing of Mutant Ataxin-3 Mediates Neuroprotection in a Rat Model of Machado-Joseph Disease PloS One. Oct, 2008 | Pubmed ID: 18841197 Striatal and Nigral Pathology in a Lentiviral Rat Model of Machado-Joseph Disease Human Molecular Genetics. Jul, 2008 | Pubmed ID: 18385100 Liposomal and Viral Vectors for Gene Therapy of the Central Nervous System Current Drug Targets. CNS and Neurological Disorders. Aug, 2005 | Pubmed ID: 16101560 Molecular Targets and Therapeutic Strategies in Huntington's Disease Current Drug Targets. CNS and Neurological Disorders. Aug, 2005 | Pubmed ID: 16101555 Lentiviral-mediated Delivery of Mutant Huntingtin in the Striatum of Rats Induces a Selective Neuropathology Modulated by Polyglutamine Repeat Size, Huntingtin Expression Levels, and Protein Length The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. May, 2002 | Pubmed ID: 11978824 Isolation of Adeno-associated Viral Vectors Through a Single-step and Semi-automated Heparin Affinity Chromatography Protocol Miguel M. Lopes*1,2,3, Sara M. Lopes*1,2,3, Rafael Baganha1,2,4, Carina Henriques1,2,5, Ana C. Silva1,2,3, Diana D. Lobo1,2,3, Luísa Cortes1,2,3,6, Luís Pereira de Almeida*1,2,4,5, Rui Jorge Nobre*1,2,3,4 1CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, 2CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3IIIUC - Institute for Interdisciplinary Research, University of Coimbra, 4ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, 5FFUC - Faculty of Pharmacy, University of Coimbra, 6MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra JoVE 66550 Bioengineering
Isolation of Adeno-associated Viral Vectors Through a Single-step and Semi-automated Heparin Affinity Chromatography Protocol Miguel M. Lopes*1,2,3, Sara M. Lopes*1,2,3, Rafael Baganha1,2,4, Carina Henriques1,2,5, Ana C. Silva1,2,3, Diana D. Lobo1,2,3, Luísa Cortes1,2,3,6, Luís Pereira de Almeida*1,2,4,5, Rui Jorge Nobre*1,2,3,4 1CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, 2CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3IIIUC - Institute for Interdisciplinary Research, University of Coimbra, 4ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, 5FFUC - Faculty of Pharmacy, University of Coimbra, 6MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra JoVE 66550 Bioengineering
