A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

April 21st, 2023

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations (Video) | JoVE

01:54 min

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Lysis and Digestion of Scarce Cells (Video) | JoVE

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Ligation and Decrosslinking of Scarce Cell DNA (Video) | JoVE

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Sonication and Biotin Pull-Down of Scarce Cells DNA (Video) | JoVE

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DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA (Video) | JoVE

03:34 min

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Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library (Video) | JoVE

02:37 min

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Llorenç Rovirosa*¹, Laureano Tomás-Daza*^1,2, Blanca Urmeneta¹, Alfonso Valencia^2,3, Biola M. Javierre^1,4

¹Josep Carreras Leukaemia Research Institute, ²Barcelona Supercomputing Center, ³Catalan Institution for Research and Advanced Studies (ICREA), ⁴Institute for Health Science Research Germans Trias i Pujol

* These authors contributed equally

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يتم تنظيم التعبير الجيني من خلال تفاعلات محفزات الجينات مع العناصر التنظيمية البعيدة. هنا ، نحدد كيف يسمح انخفاض الإدخال Capture Hi-C (liCHi-C) بتحديد هذه التفاعلات في أنواع الخلايا النادرة ، والتي كانت غير قابلة للقياس في السابق.

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A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

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