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Molecular Biology

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DNA Replication

How SSB Proteins Protect DNA Strands

Description

Single-strand DNA-binding proteins, or SSB proteins, help protect DNA during replication. When double-stranded DNA is unwound, the two strands separate. SSB proteins bind to the exposed single strands and keep them stable.

These proteins bind in a sequence-independent manner. That means the order...

Transcript

解旋酶解开DNA后,所产生的分离的单链DNA分子可通过链内配对形成发夹环结构,或通过链间配对形成双链DNA。 

这些链也容易受到核酸酶的攻击,从而导致DNA被降解。

为防止这种情况发生,单链DNA结合蛋白(SSB)会以连续的方式结合到单链DNA上,新进入的SSB结合在已有SSB的相邻位置。

SSB蛋白的协同结合可拉直并保护亲本DNA链,防止发夹环结构的形成或DNA重新缠绕恢复为双链结构。

SSB 蛋白紧密地结合在 DNA 的糖-磷酸骨架上,使碱基能够与互补的核苷酸结合,从而促进子链的形成。

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