Limit of Detection and Quantification

The limit of detection, or LOD, is the smallest amount of analyte that can be distinguished from background noise. In measurement science, background noise is the natural signal in a sample that can interfere with the analyte signal. The LOD helps show whether a substance is present, but it does not usually give a reliable a...

背景噪声是任何干扰分析物信号检测的测量所固有的。为了分析测得的信号是来自分析物还是仅来自噪声，计算检测限。

检测限是检测到的最低分析物量，其信号与背景噪声不同。LOD 是根据空白信号的标准偏差和校准曲线的斜率计算的。

该值对应于分析物信号是空白信号标准偏差三倍的浓度。低于此值时，无法区分分析物信号与背景噪声。

同样，定量限值对应于分析物信号是空白信号标准偏差的十倍的浓度。

LOQ 是仪器能够以合理的准确度和精密度定量的最低分析物量。