Name: choosing light sources for aas measurements
Uploaded: 2024-12-05T18:32:14.000Z
Duration: 1 min 13 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Earth and Space Science

Space Systems

3.3.9-12.B

atomic-absorption-spectroscopy-radiation-and-light-sources

Atomic Absorption Spectroscopy: Radiation and Light Sources

为了使原子吸收光谱法（AAS）遵循比尔-朗伯定律，辐射光源发射的波长范围应比待测原子吸收的波长范围更窄。
两种常见的窄范围“谱线”光源是空心阴极灯（HCLs）和无极放电灯（EDLs）。
空心阴极灯（HCL）具有一个圆柱形的空心阴极，阴极表面涂有所要分析的元素，以及一个钨或锆阳极，整个结构密封在充满低压惰性气体的玻璃管内。
在电极间施加电压会使惰性气体原子电离，这些高能粒子轰击阴极涂层，从而溅射出部分原子。
进一步的碰撞会激发部分溅射原子，这些原子通过发射特征波长的辐射而回到基态。
某些元素需要更强且更精确的空心阴极灯光源——一种充满惰性气体以及待测元素或其盐类的密封石英管。
管内强烈的射频或微波辐射使原子汽化并激发，导致其发射特征光谱。

原子吸收光谱法

原子吸收光谱法（AAS）通常会依赖于比尔-朗伯定律，这一定律需要辐射源通过发射窄范围波长的方式来匹配分析物原子的吸收特征。在原子吸收光谱中，选择合适辐射源的主要标准是：在特定波长中提供精确和强烈的辐射，从而使其能够对分析物进行准确检测。


 原子吸收光谱中通常会使用两种常见的窄范围“线”源是空心阴极灯（HCL）和无电极放电灯（EDL），它们是根据正在分析的元素和所需强度来进行选择的。


 空心阴极灯（HCL）：空心阴极灯被广泛应用于检测中等强度的元素。空心阴极灯是由一个涂有分析物元素的圆柱形空心阴极和钨阳极或锆阳极所组成的。它们被装在一个充满低压惰性气体的玻璃管中。当在电极上施加电压时，惰性气体原子将会被电离，从而导致它们轰击阴极。这会使其在“溅射”的过程中击出分析物原子，并发射出针对特定元素的辐射。由于空心阴极灯具有可靠性和特异性的特性，因此空心阴极灯是对原子吸收光谱进行常规测量的理想选择。


 无极放电灯（EDL）：可以将无极放电灯应用于需要更加精确或更加强烈的辐射的元素。无极放电灯有一个密封的石英管，其中包含惰性气体和分析物元素或其中的盐类。通过施加一个较强的射频或微波场，分析物将会被蒸发和激发，并发出特征光谱。无极放电灯能够提供比空心阴极灯更加强烈的辐射，这使其能够适用于需要高灵敏度和高精度的元素。


 空心阴极灯和无极放电灯都是根据原子吸收光谱分析中的具体要求来进行选择的，这确保了发射的辐射能够与分析物的吸收特性相匹配，并以此来实现准确的测量。

原子吸收光谱法（AAS）依赖于比尔-朗伯定律，该定律要求辐射源发射出与待测元素吸收线相匹配的窄波长范围的光。

Education

教育

JoVE Core

Analytical Chemistry

分析化学

Unit 1

第一单元

Atomic Spectroscopy

原子光谱

KEY TERMS AND CONCEPTS

关键术语与概念

choosing light sources for aas measurements

Atomic absorption spectroscopy (AAS) uses a light source that emits a very narrow range of wavelengths. This is needed because the source must match the absorption behavior of the analyte atom. The choice of source also depends on giving a precise, intense emission at the right wavelength for accurate detection.

Two common line sources in AAS are hollow-cathode lamps (HCLs) and electrodeless-discharge lamps (EDLs). These lamps are selected based on the element being analyzed and the level of intensity needed. Each one helps the instrument measure the analyte by sending out element-specific radiation.

Hollow-cathode lamps are widely used when moderate intensity is enough. An HCL has a cylindrical hollow cathode coated with the analyte element and a tungsten or zirconium anode. The parts sit inside a glass tube filled with an inert gas at low pressure. When voltage is applied, the gas atoms become ionized and strike the cathode. This causes sputtering, which knocks analyte atoms off the cathode and makes them emit radiation specific to that element.

Electrodeless-discharge lamps are used when more precise or more intense radiation is needed. An EDL contains a sealed quartz tube with an inert gas and the analyte element or its salt. A strong radio-frequency or microwave field vaporizes and excites the analyte. The excited atoms then emit their characteristic spectrum. EDLs give stronger emission than HCLs, so they are useful when higher sensitivity and precision are needed. Both lamp types are chosen to make sure the emitted radiation matches the analyte absorption in AAS measurements.

Choosing Light Sources for AAS Measurements

choosing-light-sources-for-aas-measurements

atomic spectroscopy: absorption, emission, and fluorescence

atomic-spectroscopy-absorption-emission-and-fluorescence

Element Analysis with Atomic Light Spectra

Atomic spectroscopy is used to identify and measure elements by their light signals. It supports both qualitative analysis, which tells what is present, and quantitative analysis, which tells how much is present. The optical methods in this group are atomic absorption spectroscopy, atomic emission spectroscopy, and atomic fluorescence spectroscopy. These methods begin with atomization. In this step, a solid, liquid, or solution sample is changed into gas-phase atoms and ions. Once the sample...

atomic spectroscopy: effects of temperature

atomic-spectroscopy-effects-of-temperature

How Heat Changes Atomic Spectra

Heat changes atomic spectra by affecting atomization, excitation, ionization, and line shape. In atomic spectroscopy, atomization means converting a sample into gas-phase atoms and ions. The flame temperature needed for this step changes how efficiently the method works and shifts the balance between excited and ground-state atoms. At thermal equilibrium, the relative numbers of excited and ground-state atoms can be estimated with the Maxwell-Boltzmann distribution. A small temperature rise...

atomic absorption spectroscopy: overview

Atomic Absorption Spectroscopy for Trace Metals

Atomic absorption spectroscopy (AAS) measures trace metals by tracking how atoms absorb electromagnetic radiation (EMR). The absorbed light makes atoms move to a higher-energy orbit. In AAS, the key step is atomization, which converts the sample into gas-phase atoms. A flame or a furnace is typically used for this step. Inside the flame, some atoms become thermally excited. Most stay in the ground state, which is the lowest-energy state. These ground-state gas-phase atoms absorb EMR only when...

atomic absorption spectroscopy: instrumentation

atomic-absorption-spectroscopy-instrumentation

Atomic Absorption Spectroscopy: Key Parts

Atomic absorption spectrophotometry uses a set of key parts to measure light absorbed by atoms. The main components are a radiation source, an atomizer, a monochromator, and a detector. Each part has a specific job in the instrument. The radiation source is usually a hollow-cathode lamp, or HCL, or an electrodeless-discharge lamp, or EDL. Both produce a narrow emission line at the needed wavelength. Some instruments instead use continuum sources with high-resolution monochromators to narrow...

atomic absorption spectroscopy: radiation and light sources

Atomic absorption spectroscopy (AAS) uses a light source that emits a very narrow range of wavelengths. This is needed because the source must match the absorption behavior of the analyte atom. The choice of source also depends on giving a precise, intense emission at the right wavelength for accurate detection. Two common line sources in AAS are hollow-cathode lamps (HCLs) and electrodeless-discharge lamps (EDLs). These lamps are selected based on the element being analyzed and the level of...

atomic absorption spectroscopy: atomization methods

atomic-absorption-spectroscopy-atomization-methods

Atomic Absorption Spectroscopy: Sample Atomization

Atomic absorption spectroscopy uses sample atomization to turn a liquid specimen into free atoms for analysis. The two main approaches are flame atomization and electrothermal atomization. Both methods prepare the sample so its atoms can be measured by the instrument. Flame atomization uses a nebulizer and spray chamber to mix the sample with a fuel-oxidant mixture. This creates a fine aerosol mist that enters the burner. The fuel and oxidant are usually close to a stoichiometric ratio, but a...

atomic absorption spectroscopy: interference

atomic-absorption-spectroscopy-interference

Correcting Errors in Atomic Absorption Spectroscopy

Atomic absorption spectroscopy interference can create systematic error by making the analytical signal or the background look stronger or weaker than it should be. In atomic absorption measurements, these errors are usually grouped as spectral interference, chemical interference, and physical interference. Spectral interference happens when signals from other elements or molecules overlap with the analyte signal, the substance being measured. This overlap can falsely raise or hide the analyte...

atomic absorption spectroscopy: lab

AAS Sample Prep and Calibration

Atomic absorption spectroscopy (AAS) depends on careful sample preparation and calibration. Samples must be turned into clear solutions before measurement. That often means extra treatment to dissolve materials such as soils, animal tissues, and minerals. Common sample prep methods include hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, and fusion with reagents. These steps help make the sample suitable for AAS analysis. Solutions that contain organic...

atomic emission spectroscopy: overview

Atomic Emission Spectroscopy for Element Analysis

Atomic emission spectroscopy is used to identify the elements in a sample by measuring the light they emit. Atoms are first excited to higher energy levels by thermal energy from hot sources such as plasma, arcs, or sparks. When they fall back to lower energy levels, they give off light at specific wavelengths. Each element produces its own set of lines, so the emission spectrum can show both what is present and how much is there. AES instruments are similar to atomic absorption spectrometers,...

atomic emission spectroscopy: instrumentation

atomic-emission-spectroscopy-instrumentation

AES Sample Handling and Detection

Atomic emission spectrometry (AES) uses sample handling, atomization, and detection parts to measure emitted light from atoms and ions. The sample must first be changed into a gas-phase form so it can produce a measurable signal. AES uses two main atomizer types. Continuous atomizers, such as flames and plasmas, feed the sample in a steady stream. Discrete atomizers add one sample at a time with syringes or autosamplers. The electrothermal atomizer is the most common discrete type. Many...

atomic emission spectroscopy: interference

atomic-emission-spectroscopy-interference

Reducing Interference in AES Signals

Atomic emission spectroscopy (AES) can produce interfering signals when atoms and molecules in a flame or plasma emit overlapping light. High-temperature atomizers excite many species at once, including oxides, hydroxides, and flame combustion products. These extra emissions can make the analyte signal harder to read. Spectral interference happens when emission lines or bands overlap. One way to reduce it is to improve instrument resolution. Another is to choose a different emission line for...

inductively coupled plasma atomic emission spectroscopy: principle

inductively-coupled-plasma-atomic-emission-spectroscopy-principle

ICP-AES Torch and Sample Analysis

Inductively coupled plasma atomic emission spectroscopy uses a plasma torch to analyze elements in a sample. This method is also called inductively coupled plasma optical emission spectroscopy, or ICP-OES. It is widely used because the plasma creates a strong environment for elemental analysis. The ICP source, or torch, contains three concentric quartz tubes. Argon gas flows through the tubes. A spark from a Tesla coil starts the ionization of argon and creates a high-temperature plasma. The...

inductively coupled plasma atomic emission spectroscopy: instrumentation

inductively-coupled-plasma-atomic-emission-spectroscopy

ICP-AES Instrument Types and Detection

Inductively coupled plasma atomic emission spectroscopy, or ICP-AES, uses an inductively coupled plasma to detect and analyze elements in a sample. The method measures the light given off by atoms, which helps identify and compare different elements. It is a common approach in atomic emission spectroscopy. ICP-AES instruments are built in three main forms: sequential, simultaneous multichannel, and Fourier transform instruments. Fourier transform designs are less commonly used, but they are...

atomic emission spectroscopy: lab

Plasma Sources in Atomic Emission Spectroscopy

Atomic emission spectroscopy uses plasma sources to identify elements by their light. The method creates abundant spectra with characteristic emission lines. Inductively coupled plasma, or ICP, is especially useful because it gives strong quantitative data. It works well under stable conditions, with low noise, low background, and few interferences. Newer air-operated microwave sources are also being studied. These sources may be more cost-effective than conventional ICP systems. Atomic...

atomic fluorescence spectroscopy

Measuring Elements by Atomic Fluorescence

Atomic fluorescence spectroscopy measures elements by detecting the light atoms give off after they are excited by electromagnetic radiation. The atoms are first placed in a flame, furnace, or plasma. They absorb energy, become excited, and then release that energy as they return to their original state. The emitted light is called fluorescence. The fluorescence is observed at a right angle to the incoming beam. This setup helps separate the emitted light from the light used to excite the...

flame photometry: overview

Flame Emission Spectrometry Basics

Flame emission spectrometry uses a flame to excite elements in a sample so their light can be measured. It is also called flame photometry. The method can be used for both qualitative and quantitative analysis of elements. The idea became established in the early 1860s when Kirchhoff and Bunsen showed that specific elements give off characteristic radiation in a flame. The first instrument for this purpose measured sodium in plant ash with a Bunsen flame. A major problem was how to add the...

flame photometry: lab

Flame Photometry and Metal Detection

Flame photometry is a method for detecting metals by measuring the light they emit in a flame. A solution such as potassium chloride is aspirated into the flame. The solvent evaporates first. The remaining salt becomes dehydrated and then dissociates into free gaseous atoms in the ground state. Some of these atoms absorb energy from the flame and move to an excited state. When they return to the ground state, they emit photons at characteristic wavelengths. These emissions are very narrow...

key terms and concepts

Learn how AAS uses hollow-cathode lamps and electrodeless-discharge lamps to match narrow wavelengths for accurate analyte detection and measurement.

Choosing Light Sources for AAS Measurements | JoVE K12

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3.3.9-12.D

Choosing Light Sources for AAS Measurements

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