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Home
JoVE Core
Molecular Biology
互补测试
Video Quiz
互补测试
JoVE Core
Molecular Biology
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JoVE Core Molecular Biology
Complementation Tests

16.4: 互补测试

6,356 Views
00:49 min
April 7, 2021
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Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

互补测试是一种简单的杂交,以确定两个突变是否位于同一基因或不同基因上。 20 世纪 40 年代,爱德华·刘易斯 (Edward Lewis) 在研究果蝇时首次进行了这项技术。 他开发了该测试来识别染色体上不同突变的位置和排列。

不同突变的杂合生物在所有组合中都是成对杂交的。 如果存在于不同的基因上,突变可以通过提供产生野生型表型的缺失遗传元件来相互补充。 然而,如果两个突变属于同一基因,则它们不能互补并且不能表现出野生型表型。 不能相互互补的突变被认为属于同一互补组。 相互互补的突变属于不同的互补组。

Transcript

有时,不同的突变可以产生相同的表型,例如鸟类羽毛的着色丧失。这些突变是在同一基因中还是在两个不同的基因中,可以使用互补试验来确定。

考虑两只突变鸡之间的繁殖。如果同一个基因在父母双方都发生了突变,后代将继承突变并呈现白色。

另一方面,如果后代像野生鸟一样呈棕色,那么这表明父母在两个不同的基因中发生了突变。

尽管每个亲本基因都有一个突变,但它们共同产生了野生型表型。这种现象称为互补。

Explore More Videos

互补试验 突变 同基因 异基因 Edward Lewis 果蝇 染色体 杂合子 杂交 补体 野生型表型 互补组

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