Frequency Dissipation Shift
Frequency Dissipation Shift - Related Videos
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Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction
0 Views •
2025
This video demonstrates a dissipative microgravimetry technique to investigate the binding of proteins to lipid bilayers. Small unilamellar vesicles are added to coat the surface of the quartz sensor of a microbalance. Utilizing the calcium ion-dependent binding of the target phospholipid-binding protein to the bilayer, changes in the oscillation frequency of the sensor and the dissipation of the oscillation are monitored to determine the nature of the protein-lipid bilayer binding.
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Sound Waves and Doppler Shift
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Source: Arianna Brown, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA Waves are disturbances that propagate through a material medium or empty space. Light waves can travel through a vacuum and some forms of matter, and are transverse in nature, which means that the oscillations are perpendicular to the direction of propagation. However, sound waves are pressure waves that travel through an elastic medium like air,...
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The equivalent resistance of a combination of resistors depends on their values and how they are connected. The simplest combinations of resistors are series and parallel connections. In a series circuit, the first resistor's output current flows into the second resistor's input; therefore, each resistor's current is the same. Thus, the equivalent resistance is the algebraic sum of the resistances. The current through the circuit can be found from Ohm's law and is equal to the battery's emf...
06:57
Electrophoretic Mobility Shift Assay (EMSA)
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The electrophoretic mobility shift assay (EMSA) is a biochemical procedure used to elucidate binding between proteins and nucleic acids. In this assay a radiolabeled nucleic acid and test protein are mixed. Binding is determined via gel electrophoresis which separates components based on mass, charge, and conformation. This video shows the concepts of EMSA and a general procedure, including gel and protein preparation, binding, electrophoresis, and detection. Applications covered in this video...
09:45
New Variations for Strategy Set-shifting in the Rat
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Cited by 3 •
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Set-shifting, a form of behavioral flexibility, requires an attentional shift from one stimulus dimension to another. We extended an established rodent set-shifting task1 by requiring attention to different stimuli according to context. The task was combined with specific lesions to identify neuron subtypes underlying a successful shift.
FAQs
A frequency shift primarily reports changes in the material coupled to the oscillating sensor, including added mass and interfacial loading. In a biochemical experiment, binding at the surface can therefore alter the resonance response even when the bound material is part of a larger interfacial layer rather than a simple, isolated molecule.
The dissipation shift adds mechanical information that frequency alone cannot provide. A larger energy loss indicates that the surface-associated layer deforms more readily during oscillation, consistent with viscoelastic behavior. This helps identify biochemical films that are soft or hydrated, rather than interpreting every frequency change as a purely mass-related event.
Interpreting the two responses together helps separate rigid accumulation from changes in layer mechanics. A frequency response accompanied by little energy loss is consistent with a more rigidly coupled surface contribution, whereas a stronger dissipation response points to a deformable or reorganizing layer. This comparison is especially useful during biomolecular binding and membrane formation.
The relative size and evolution of the two shifts provide clues about what happens after initial attachment. A layer may continue reorganizing at the interface, changing its deformation and energy-loss behavior without being described only by the amount of material present. Tracking both signals therefore gives a richer picture of surface-layer restructuring.
Measurements follow the resonance behavior and energy-loss response as material binds to, or otherwise alters, the sensor surface. Recording the paired shifts over time allows investigators to relate signal changes to biochemical events such as adsorption, binding, membrane formation, or subsequent layer reorganization. The time-resolved record is important because these processes can change the interfacial film.
Protein adsorption is one important application because the paired response can indicate both accumulation at the surface and the mechanical character of the resulting film. The same approach can examine biomolecular binding and membrane formation, while changes in dissipation help reveal whether the layer is relatively rigid, soft, hydrated, or undergoing structural reorganization.