Nickel-nta Imac
Nickel-nta Imac - Related Videos
08:40
Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules
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Cited by 3 •
2014
This article describes the preparation of well-ordered nickel nanofoams via electroless metal deposition onto nanoporous templates obtained from self-assembled diblock copolymer based supramolecules.
09:02
Synthesis of Platinum-nickel Nanowires and Optimization for Oxygen Reduction Performance
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2018
The protocol describes the synthesis and electrochemical testing of platinum-nickel nanowires. Nanowires were synthesized by the galvanic displacement of a nickel nanowire template. Post-synthesis processing, including hydrogen annealing, acid leaching, and oxygen annealing were used to optimize nanowire performance and durability in the oxygen reduction reaction.
03:54
Nickel Affinity Chromatography-Based Protein Purification: A Technique to Purify Polyhistidine-Tagged Recombinant Proteins from Bacterial Cell Lysate
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2025
In this video, we demonstrate the nickel affinity chromatography technique to purify histidine-tagged pyrophosphokinase enzymes from Clostridium difficile bacteria.
07:57
Effect of Microwave Synthesis Conditions on the Structure of Nickel Hydroxide Nanosheets
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Cited by 1 •
2023
Nickel hydroxide nanosheets are synthesized by a microwave-assisted hydrothermal reaction. This protocol demonstrates that the reaction temperature and time used for microwave synthesis affects the reaction yield, crystal structure, and local coordination environment.
09:35
Applying Dynamic Strain on Thin Oxide Films Immobilized on a Pseudoelastic Nickel-Titanium Alloy
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2020
Dynamic, tensile strain is applied on TiO2 thin films to study the effects of strain on electrocatalysis, specifically proton reduction and water oxidation. TiO2 films are prepared by thermal treatment of the pseudo-elastic NiTi alloy (Nitinol).
FAQs
Selectivity comes from coordination between histidine residues in the polyhistidine tag and immobilized nickel. The nickel is not free in solution; nitrilotriacetic acid groups hold it on the resin, creating a localized binding site. During washing, this arrangement helps retain the tagged recombinant protein while unbound or weakly associated lysate components are removed.
Nitrilotriacetic acid groups anchor nickel ions to the chromatography resin, preventing the metal from dispersing during purification. The immobilized nickel then provides the coordination site recognized by histidine residues on the tagged protein. Together, the chelator, metal ion, and resin connect molecular recognition with physical separation from a complex biochemical mixture.
Elution occurs when the interaction between the tagged protein and immobilized nickel is disrupted. Imidazole provides one route for releasing the target, while altered buffer conditions provide another. Because the protein is recovered after contaminants have been washed away, this step produces an enriched preparation suitable for subsequent biochemical analyses.
A typical workflow applies a complex lysate to the nickel-NTA resin so the polyhistidine-tagged protein can bind, washes the resin to remove contaminants, and then elutes the retained protein. This sequence separates capture from recovery and explains why the method can rapidly isolate a recombinant target from a heterogeneous biochemical sample.
The method is particularly useful when a recombinant protein contains a polyhistidine tag and must be isolated from a complex lysate. Its rapid format supports purification before additional experiments, while its scalability makes it relevant across workflows that require either small-scale biochemical preparation or larger amounts of purified target protein.
Purified proteins obtained by this approach can be used before structural, enzymatic, or binding analyses. In biochemistry, that connection is important because these studies require an isolated target rather than an unresolved lysate mixture. Nickel-NTA IMAC therefore functions as an upstream preparation step linking recombinant expression to molecular characterization.