Neonatal Rat Cardiomyocytes
Neonatal Rat Cardiomyocytes - Related Videos
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
13:36
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
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Cited by 12 •
2014
This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.
09:35
Neurodevelopmental Reflex Testing in Neonatal Rat Pups
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Cited by 77 •
2017
Behavioral testing is the gold standard for determining outcomes following brain injury, and can identify the presence of developmental disabilities in infants and children. Neurodevelopmental reflexes are an early indicator of these abnormalities. A host of easily accomplished developmental reflex tests in the neonatal rodent were developed and described here.
08:03
Recording EEG in Freely Moving Neonatal Rats Using a Novel Method
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Cited by 3 •
2017
Here, we introduce a novel technique designed to record electroencephalography (EEG) in freely moving neonatal epileptic pups and describe its procedures, features, and applications. This method allows one to record EEG for more than 1 week.
FAQs
Rhythmic contraction depends on coordinated electrical coupling and intracellular calcium cycling. In culture, electrical activity supports communication among cells, while calcium movement within cells helps regulate the contractile response. Together, these processes make spontaneous beating a functional readout for studying how bioengineered environments influence cardiac behavior over time.
Adhesion to the culture surface is an important early condition because it allows cells to remain organized enough for electrical coupling and calcium cycling to be observed. In bioengineering experiments, changes in adhesion can affect whether a material supports stable, measurable contractile behavior in culture and can therefore influence evaluation of material compatibility.
Mechanical, chemical, and electrical cues can each alter the contractile behavior of cultured cells, but they probe different aspects of cardiac response. Comparing these inputs helps researchers determine whether a biomaterial, stimulation strategy, or treatment changes contraction, calcium-related activity, or other indicators of functional adaptation relevant to engineered cardiac systems.
Preparation begins with isolating the cells from neonatal myocardium through enzymatic dissociation. The resulting cells are then maintained in culture so that adhesion, electrical coupling, intracellular calcium cycling, and rhythmic contraction can be assessed. This workflow creates a controlled platform for testing bioengineering conditions before applying findings to more complex cardiac models.
These cells support testing of biomaterials, engineered cardiac tissues, electrical stimulation strategies, and therapies intended to improve myocardial performance. Their contractile behavior provides a functional response while their sensitivity to environmental cues helps investigators examine whether a design promotes cardiac activity or remains compatible with living cardiac cells.
Measurements of contractile behavior and responses to mechanical, chemical, or electrical cues can reveal effects on cardiac function, tissue maturation, drug activity, and device compatibility. Results help researchers compare candidate designs or interventions in a focused culture system and decide which findings warrant progression toward more complex models and translational applications.