Amphibian Model
Amphibian Model - Related Videos
11:39
Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
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Cited by 29 •
2013
We describe the preparation of thin retinal slices from aquatic tiger salamanders (Ambystoma tigrinum) and explain how we use these slices to study synaptic processing in the retina by obtaining dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells.
06:49
Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
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Cited by 18 •
2014
Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment protocol.
04:37
Reproductive Techniques for Ovarian Monitoring and Control in Amphibians
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Cited by 17 •
2019
The study of amphibian biology provides valuable information on the reproductive, physiological, embryological and developmental processes that drive organisms of many taxonomic groups. Here, we present a comprehensive guide on different methodologies that can be used to study ovarian control and monitoring in amphibians.
05:19
Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
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2025
This is a method for sampling and isolating culturable bacteria from the cutaneous microbiota of European plethodontid salamanders (Speleomantes genus). Here, we present a protocol for sampling salamander skin with swabs and processing them using culturable approaches. We describe sampling, isolation, and establishment of axenic culture with bacterial strain characterization.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
FAQs
Because embryos develop outside the parent, researchers can examine developmental changes under controlled laboratory or field conditions and relate visible alterations in form to underlying cellular mechanisms. This accessibility supports investigations of embryonic development and gene function, while allowing biological processes to be connected with changes that can be observed directly during early growth.
Metamorphosis provides a naturally coordinated transition in form and function that is regulated by hormones. Studying this process allows researchers to investigate endocrinology alongside developmental biology, linking hormonal control with visible biological changes. The model is therefore useful for examining how physiological regulation contributes to major developmental transitions in vertebrate organisms.
Some amphibian species can regenerate damaged tissues, creating an experimental context for examining how injury is followed by tissue restoration. Researchers can use this capacity to connect cellular mechanisms with changes in structure and function. These observations contribute to regeneration studies and may also provide comparative context for biology and biomedical research.
Their value comes from combining experimentally accessible developmental stages with vertebrate physiology. Researchers can investigate processes such as gene function, hormonal regulation, or tissue regeneration and then relate those mechanisms to visible changes in form and function. This connection helps place cellular findings within broader biological processes rather than studying them in isolation.
A study begins by selecting an appropriate living amphibian or amphibian-derived system for the biological question, followed by observation under controlled laboratory or field conditions. Researchers then examine relevant developmental, physiological, regenerative, or disease-related changes and interpret the results in relation to the process being investigated. The specific workflow depends on the research objective.
Researchers may choose this approach when they need experimentally accessible development together with vertebrate physiology. External embryos can support developmental studies, while metamorphosis and regeneration provide additional biological processes to examine. This combination makes amphibian systems useful when the question requires links between cellular mechanisms, organismal changes, and physiological regulation.
Applications include embryonic development, gene function, endocrinology, regeneration, environmental toxicology, and disease biology. Findings can also support comparative biology and conservation by revealing how biological processes operate in amphibians and relate to broader vertebrate systems. In biomedical research, the models provide context for studying mechanisms that connect cellular activity with tissue or organismal outcomes.