Fura-2 Dye
Fura-2 Dye - Related Videos
12:15
Calcium Imaging of Cortical Neurons using Fura-2 AM
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Cited by 108 •
2009
Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.
05:58
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells
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2019
Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed.
08:29
Assessment of Myofilament Ca2+ Sensitivity Underlying Cardiac Excitation-contraction Coupling
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Cited by 9 •
2016
This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
FAQs
Binding Ca2+ alters the dye’s excitation spectrum, changing the relative fluorescence obtained when cells are illuminated near 340 and 380 nm. By tracking these wavelength-dependent responses and collecting emission near 510 nm, investigators can follow calcium-dependent signal changes without treating a single intensity value as the complete measurement. This spectral behavior supports measurements in living cells.
A single fluorescence intensity can vary if dye concentration, illumination, or cell thickness changes. Comparing signals generated by excitation near 340 and 380 nm makes the measurement less dependent on those influences because the result is based on their relationship. The resulting ratio provides a more reliable way to follow calcium signals under changing experimental conditions.
Emission near 510 nm provides a common detection signal for both excitation conditions. Measuring fluorescence in the same emission region allows researchers to compare the two excitation responses rather than combining differences in detection with changes caused by Ca2+ binding. This arrangement helps relate the measured ratio to intracellular calcium-related changes.
The dye first enters the cells, after which fluorescence is recorded while the sample receives excitation near 340 and 380 nm. Emission near 510 nm is monitored for each excitation condition, and the resulting signals are compared as a ratio. Repeating these measurements over time allows researchers to observe rapid changes in cytosolic calcium.
Changes in the fluorescence ratio provide a time-sensitive readout of changing cytosolic calcium. Because calcium participates in intracellular signaling, the measurement can reveal when calcium-dependent activity rises or changes during a biological response. This temporal information is useful for connecting cellular signals with physiological events rather than simply describing how bright a cell appears.
Fura-2 measurements support studies of neuronal activity, muscle contraction, secretion, and other calcium-dependent processes. In each setting, researchers can monitor intracellular calcium changes as part of the response being studied. The approach is therefore useful across biology when calcium signals help explain how cells communicate, respond to stimulation, or carry out specialized functions.
Its ratiometric measurement links a calcium-sensitive spectral response with reduced sensitivity to dye amount, illumination, and cell thickness. That combination helps researchers distinguish calcium-related changes from some sources of optical variation. In biological experiments, the resulting readout can clarify how intracellular calcium dynamics contribute to signaling pathways and physiological activities in living cells.