Gene-based Medicine
Gene-based Medicine - Related Videos
16:19
Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
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Cited by 5 •
2008
In this video, we show a procedure for an accurate biolistic delivery of reagents into live tissue with a novel miniature gene gun. We are knocking down the expression of the axon guidance molecule Netrin in leech embryos by delivering molecules of dsRNA into the ventral body wall and ganglia of single segments.
08:07
Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
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Cited by 6 •
2017
Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines.
06:14
Automating Tumor Implantation in Zebrafish Larvae for Cancer Research and Medicine
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2025
Here, we present a detailed protocol for automated tumor xenograft implantation into zebrafish larvae using a state-of-the-art robotic injection system. Furthermore, we present injection videos and validation data demonstrating the system's successful performance.
01:29
Microorganisms in Medicine and Therapeutics
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2026
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
04:24
Acupoint Application as a Traditional Chinese Medicine Treatment for Fatigue Associated with Chronic Obstructive Pulmonary Disease
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2025
This protocol describes the specific process and efficacy of acupoint application in treating fatigue symptoms associated with chronic obstructive pulmonary disease.
FAQs
These strategies act on genetic information in different ways. Replacement addresses a faulty gene, whereas addition supplies a functional copy without necessarily removing the original sequence. Silencing reduces harmful gene activity, and genome editing changes a DNA sequence directly. Selecting among them depends on whether disease results from missing function, harmful activity, or an altered sequence.
Nucleic acids must reach the cells in which their genetic effects are needed, making delivery a central part of treatment design. Viral and nonviral vectors transport this material into target cells, where it can support gene addition, silencing, replacement, or editing. Improvements in delivery can therefore expand which diseases and cell types are accessible to therapy.
Genetic variation can affect both disease biology and the suitability of a particular intervention. Molecular information helps identify whether a condition involves a faulty gene, harmful gene activity, or another sequence change, guiding the selection of replacement, addition, silencing, or editing. This relationship also supports personalized diagnosis and therapy selection rather than relying on one approach for every patient.
Genetic information can connect an individual’s molecular profile with the biological basis of disease. That information may help distinguish relevant genetic changes and inform which therapeutic strategy is most appropriate. In this way, gene-based medicine contributes not only to treatment but also to more individualized diagnosis and decisions about therapy selection.
The disease context determines which molecular problem must be addressed and which cells require intervention. Inherited disorders may involve a faulty gene that needs replacement or functional support, while cancers may involve harmful gene activity or other genetic changes. These distinctions influence the intervention selected, the delivery approach, and how outcomes are evaluated.
Safety monitoring is essential because these approaches alter or regulate genetic information within cells. Ongoing evaluation helps researchers assess how interventions perform and identify issues that may affect their use. Continued work in gene delivery, genome editing, and monitoring is expanding therapeutic potential while improving understanding of how genetic variation relates to health and disease.