Retinal Hormone Mount
Retinal Hormone Mount - Related Videos
16:47
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
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Cited by 45 •
2011
A formal demonstration of the dissection of a mouse eye, resulting in a whole mount of the retinal pigment epithelium.
02:13
Types of Hormones
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2019
Hormones can be classified into three main types based on their chemical structures: steroids, peptides, and amines. Their actions are mediated by the specific receptors they bind to on target cells. Steroid hormones are derived from cholesterol and are lipophilic in nature. This allows them to readily traverse the lipid-rich cell membrane to bind to their intracellular receptors in the cytoplasm or nucleus. Once bound, the cytoplasmic hormone-receptor complex translocates to the nucleus.
01:33
Hormonal Regulation
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2019
The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
09:20
Whole-mount Retinal Organoid Visualization with Cellular Resolution
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2025
This protocol combines optical clearing and immunolabeling for full-volume confocal imaging of whole-mount retinal organoids. It preserves 3D structure, enabling detailed visualization of key neuronal pathways, improving the study of retinal maturation, spatial organization, and its potential applications in disease modeling and personalized medicine.
01:56
Plant Hormones
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2020
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands. Instead, plant hormones are often produced in regions of active growth, like the tips of roots and shoots. Additionally, even very low concentrations of plant hormones can have a profound effect on growth and development processes.
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Flattening the retina converts its layered, curved organization into a sheet while retaining relationships among regions and cell types. That spatial preservation lets microscopy show where hormone receptors, endocrine signals, or related markers occur relative to retinal structure, rather than only indicating that a molecule is present. The result links localization with tissue organization and potential function.
Antibody-based labeling identifies selected molecular targets through their associated receptors, endocrine signals, or cellular markers. The resulting signal can be examined across the retinal sheet, allowing researchers to compare localization among regions and layers. Other stains can complement antibody labeling by revealing additional tissue features, so molecular observations remain interpretable within the preserved retinal architecture.
Spatial position can distinguish a signal associated with one retinal layer or region from a signal distributed more broadly. This matters because hormone receptors and endocrine signals are interpreted alongside the cells and structures around them. A mount therefore supports questions about organization and signaling relationships that are difficult to address from molecular detection without tissue context.
The preparation begins with retinal dissection, followed by flattening to create a tissue sheet and fixation to preserve the sample. Researchers then apply antibody-based labels or other stains and examine the preparation by microscopy. Each stage serves a different purpose: shaping the tissue, stabilizing it, marking targets, and documenting their distribution.
Retinal mounts can support comparisons involving development, physiology, and disease-related changes. Researchers can assess whether hormone receptors, endocrine signals, or associated markers differ in their location across retinal regions or in their relationship to tissue organization. Because the preparation preserves spatial arrangement, observed differences can be considered in anatomical context rather than as isolated molecular measurements.
In biology, this preparation connects neuroendocrine signaling with retinal structure. It can be used to investigate where hormone-related molecules or signaling cells occur, how those patterns relate to retinal organization, and how localization changes during development, physiology, or disease. Microscopy findings therefore provide a tissue-level view of molecular signaling and its structural context.