Slide Scanning
Slide Scanning - Related Videos
09:33
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System
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Cited by 2 •
2018
Here we describe a protocol for the automated segmentation of fluorescently labeled tissues on slides using a widefield high-content analysis system (WHCAS). This protocol has wide-ranging applications in any field which involves the quantitation of fluorescent markers in biological tissues including the biological sciences, medical engineering, and health sciences.
02:28
Leaky Scanning
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2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
01:18
Design Example: Designing Water Slide
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2025
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed. Bernoulli's principle determines the water's velocity along the slide.
06:14
In vitro Biofilm Formation in an 8-well Chamber Slide
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Cited by 62 •
2011
This article describes the procedure for the formation and visualization of a bacterial biofilm grown within an 8-well chamber...
10:04
Visualizing Soil Microorganisms via the Contact Slide Assay and Microscopy
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Soil comprises the thin layer at the earth’s surface, containing biotic and abiotic factors that contribute to life. The abiotic portion includes inorganic particles ranging in size and shape that determine the soil’s texture. The biotic portion incorporates plant residues, roots, organic matter, and microorganisms. Soil microbe abundance and diversity is expansive, as...
FAQs
A slide scanner builds a large specimen image by moving the slide through successive fields of view and capturing overlapping regions. The overlap allows the scanning system to assemble those fields into one navigable image rather than leaving separate frames. This arrangement preserves access to different specimen areas while making the biological sample viewable as a continuous digital record.
Automated illumination and optics provide the imaging conditions needed to capture high-resolution views, while automated focus adjusts image capture across the specimen. Together, these components help maintain usable detail from one field to the next. Consistent acquisition is especially valuable when biological tissues or cells must be reviewed, compared, or analyzed across a complete slide.
Digital slide imaging reduces dependence on repeated manual microscope examination by creating a persistent, navigable record of the specimen. Instead of returning to the glass slide for every review, researchers can view the captured image, share it with collaborators, and use it for documentation or analysis. The original slide remains the physical source, while the digital image expands access.
Digital slides support quantitative image analysis by providing images that can be examined systematically rather than only through direct visual inspection. In biology, this can include computational methods such as automated cell detection, alongside analysis of histological, pathological, cellular, or tissue features. These capabilities help researchers extract measurements or observations from recorded specimen images.
A typical workflow places a prepared glass microscope slide on the scanner’s motorized stage, uses automated illumination and optics to capture successive specimen fields, adjusts focus during acquisition, and assembles the overlapping fields into a single digital image. The resulting file can then be viewed, analyzed, shared, or retained as documentation for the biological specimen.
Biologists may use Slide Scanning when histology, pathology, cell biology, or tissue research requires consistent documentation, remote collaboration, or quantitative image analysis. It is also useful when many people need access to the same specimen image, such as in teaching or shared research review. The approach extends specimen access beyond the microscope stage without requiring repeated manual examination.
Digital slides provide reusable records that can support teaching and long-term archiving while making biological specimens easier to access and share. Their image format also supports computational approaches, including automated cell detection and other quantitative analyses. These uses connect routine specimen documentation with collaborative review and image-based research in histology, pathology, cell biology, and tissue studies.