Transwell Filter Seeding
Transwell Filter Seeding - Related Videos
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
01:27
Passive Filters
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2024
Passive filters are utilized to shape the frequency spectrum of signals across a diverse array of applications. These filters, using only passive elements like resistors (R), inductors (L), and capacitors (C), are capable of selectively allowing or blocking certain frequency ranges without the need for external power sources. Low-Pass Filters Low-pass filters are designed to transmit signals with frequencies lower than the cutoff frequency, ωc, and attenuate those above it. The cutoff frequency...
03:20
A Transwell Tissue Model for Studying Interactions Between Human Oral Biofilms and Epithelium
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2026
Source: Alshehri, M. et. al., Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro. J. Vis. Exp. (2025)This video demonstrates a co-culture model using multi-species oral biofilms and human oral epithelium. It highlights epithelial immune activation and enables the downstream analysis of host-pathogen interaction.
09:32
Evaluating the Effect of Coated Filters and Pre-Filters on the Infectivity and Concentration of Aerosolized Phi6 Virus
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2026
Here, we present a study to evaluate the anti-viral efficacy of copper- and silver-based surface coatings on air filters. Using a bioaerosol chamber and Phi6 bacteriophage, this method quantifies viral inactivation and mechanical filtration efficiency for self-sanitizing indoor air technologies.
01:25
Active Filters
0 Views •
2025
Active filters are electronic circuits that use operational amplifiers (op-amps), resistors, and capacitors to filter out unwanted frequency components from a signal. A first-order low-pass active filter is designed to pass signals with a frequency lower than a certain cutoff frequency and attenuate frequencies higher than that cutoff frequency. The transfer function for a first-order low-pass active filter is: Where Af is the gain at low frequencies also known as the dc gain, s is the...
FAQs
The membrane pores create a controlled exchange route rather than mixing the two cell compartments. Cells remain positioned on the upper surface, while nutrients and signaling molecules from the underlying medium can reach them through the filter. This separation lets investigators relate cellular responses to communication across the interface.
Keeping the chambers separate allows researchers to distinguish effects associated with the cells from effects associated with the underlying medium. The arrangement supports controlled analysis of signaling across the membrane, interactions between separated cell populations, and responses that depend on substances passing through the porous filter.
Once cells attach and grow across the membrane surface, researchers can examine how they organize into a barrier and how readily substances or signals influence the interface. This makes the system useful for analyzing permeability and barrier behavior in epithelial or endothelial models without placing both cell populations in the same compartment.
A typical workflow begins by preparing cells as a suspension, distributing them across the upper surface of the porous insert, and allowing attachment and growth. The underlying chamber contains medium that supplies nutrients and signaling molecules through the membrane. This sequence establishes the separated culture arrangement needed for downstream analysis.
Researchers choose this approach when they need to model an interface, keep cell populations separated, or assess communication across a membrane. It is particularly relevant for epithelial and endothelial barrier studies, tissue-interaction experiments, and investigations of how compounds or biological signals alter cellular behavior.
The setup can provide information about cell migration, barrier formation, permeability, and communication between cells in different compartments. It also supports testing how compounds or biological signals influence those behaviors. These outcomes help connect conditions at the membrane interface with changes in cellular organization or function.