Ductal Cannulation

Ductal cannulation is a minimally invasive technique that places a fine catheter into a glandular or organ duct to access its lumen, making it valuable for sampling, fluid delivery, and localized research interventions. The catheter is guided through the duct opening and advanced under visual or imaging control, allowing investigators to collect ductal contents or introduce defined solutions while limiting exposure to surrounding tissue. In cancer research, ductal cannulation supports studies of pancreatic and other duct-associated tumors by enabling localized treatment delivery, analysis of tumor-associated secretions, and investigation of how cancers alter duct structure and function. These applications can improve disease modeling and evaluation of targeted therapies.

Ductal Cannulation - Related Videos

Research

JoVE EoE - Pancreatic Cancer

Acinar-to-Ductal Metaplasia Induction: A Method to Study Acinar Cell to Ductal Cell Differentiation in 3D Ex Vivo Culture

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2023

This video describes the technique of differentiating pancreatic acinar cells to ductal cells in ex vivo 3D culture. This helps to understand the mechanisms regulating the acinar-to-ductal metaplasia (ADM), a key process in the early development of pancreatic cancer.

Intraduodenal Cannulation in a Rat Model for Immunopathology Studies

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2025

This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

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Cited by 17 •

2011

A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.

Cannulation of the Carotid Artery in a Rat Model

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2025

In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.

Isolation and Cannulation of Cerebral Parenchymal Arterioles

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Cited by 28 •

2016

This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.

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